Development and application of novel genome engineering technologies in saccharomyces cerevisiae

Microbial Production: From Genome Design to Cell Engineering

Volumn 9784431546078, Issue , 2014, Pages 53-62

  Sasano, Yu ^a   Sugiyama, Minetaka ^a   Harashima, Satoshi ^a  

^a OSAKA UNIVERSITY   (Japan)

Author keywords

Bioethanol; Biotechnological application; Chromosome splitting technology; Ethanol tolerance; Genome engineering; Genome reorganization technology; Glycerol; Saccharomyces cerevisiae

Indexed keywords

EID: 84930906307     PISSN: None     EISSN: None     Source Type: Book    
DOI: 10.1007/978-4-431-54607-8_5     Document Type: Chapter

References (14)

1. Dillon PJ, Rosen CA (1990) A rapid method for the construction of synthetic genes using the polymerase chain reaction. Biotechniques 9:298-299
2. Güldener U, Heck S, Fiedler T, Beinhauer J, Hegemann JH (1996) A new effi cient gene disruption cassette for repeated use in budding yeast. Nucleic Acids Res 24:2519-2524
3. Kim YH, Sugiyama M, Kaneko Y, Fukui K, Kobayashi A, Harashima S (2005) A versatile and general splitting technology for generating targeted YAC subclones. Appl Microbiol Biotechnol 69:65-70
4. Kim YH, Sugiyama M, Kaneko Y, Fukui K, Kobayashi A, Harashima S (2006) A polymerase chain reaction-mediated yeast artifi cial chromosome-splitting technology for generating targeted yeast artifi cial chromosomes subclones. Methods Mol Biol 349:103-115
5. Murakami K, Tao E, Ito Y, Sugiyama M, Kaneko Y, Harashima S, Sumiya T, Nakamura A, Nishizawa M (2007) Large scale deletions in the Saccharomyces cerevisiae genome create strains with altered regulation of carbon metabolism. Appl Microbiol Biotechnol 75:589-597
6. Murray AW, Claus TB, Szostak JW (1988) Characterization of two telomeric DNA processing reactions in Saccharomyces cerevisiae. Mol Cell Biol 8:4642-4650
7. Park AH, Sugiyama M, Harashima S, Kim YH (2012) Creation of an ethanol-tolerant yeast strain by genome reconstruction based on chromosome splitting technology. J Microbiol Biotechnol 22:184-189
8. Sugiyama M, Ikushima S, Nakazawa T, Kaneko Y, Harashima S (2005) PCR-mediated repeated chromosome splitting in Saccharomyces cerevisiae. Biotechniques 38:909-914
9. Sugiyama M, Yamamoto E, Mukai Y, Kaneko Y, Nishizawa Y, Harashima S (2006) Chromosomeshuffl ing technique for selected chromosomal segments in Saccharomyces cerevisiae. Appl Microbiol Biotechnol 72:947-952
10. Sugiyama M, Nakazawa T, Murakami K, Sumiya T, Nakamura A, Kaneko Y, Nishizawa M, Harashima S (2008) PCR-mediated one-step deletion of targeted chromosomal regions in haploid Saccharomyces cerevisiae. Appl Microbiol Biotechnol 80:545-553
11. Sugiyama M, Yamagishi K, Kim YH, Kaneko Y, Nishizawa M, Harashima S (2009) Advances in molecular methods to alter chromosomes and genome in the yeast Saccharomyces cerevisiae. Appl Microbiol Biotechnol 84:1045-1052
12. Surosky RT, Newlon CS, Tye BK (1986) The mitotic stability of deletion of chromosome III in yeast. Proc Natl Acad Sci USA 83:414-418
13. Ueda Y, Ikushima S, Sugiyama M, Matoba R, Kaneko Y, Matsubara K, Harashima S (2012) Largescale genome reorganization in Saccharomyces cerevisiae through combinatorial loss of minichromosomes. J Biosci Bioeng 113:675-682
14. Yamagishi K, Sugiyama M, Kaneko Y, Nishizawa M, Harashima S (2008) Construction and characterization of single-gene chromosomes in Saccharomyces cerevisiae. J Biosci Bioeng 106:563-567