Combined stool-based multiplex PCR and microscopy for enhanced pathogen detection in patients with persistent diarrhoea and asymptomatic controls from Côte d'Ivoire

Clinical Microbiology and Infection

Volumn 21, Issue 6, 2015, Pages 591.e1-591.e10

  Becker, S L ^a,b,c   Chatigre, J K ^d   Gohou, J P ^d   Coulibaly, J T ^a,b,e,f   Leuppi, R ^d,g   Polman, K ^h   Chappuis, F ⁱ   Mertens, P ^j   Herrmann, M ^c   N'Goran, E K ^e,f   Utzinger, J ^a,b   von Muller L ^c  

^a SWISS TROPICAL AND PUBLIC HEALTH INSTITUTE   (Switzerland)

^b UNIVERSITY OF BASEL   (Switzerland)

^c SAARLAND UNIVERSITY   (Germany)

^d Hôpital Méthodiste de Dabou   (Côte d’Ivoire)

^e CENTRE SUISSE DE RECHERCHES SCIENTIFIQUES EN CÔTE D'IVOIRE   (Côte d’Ivoire)

^f UNIVERSITÉ FÉLIX HOUPHOUËT BOIGNY   (Côte d’Ivoire)

^g Ruedi Leuppi Foundation Côte d'Ivoire   (Switzerland)

^h INSTITUTE OF TROPICAL MEDICINE   (Belgium)

ⁱ GENEVA UNIVERSITY HOSPITALS   (Switzerland)

^j Coris BioConcept ^*  (Belgium)

more..

Author keywords

Bacteria; C te d'Ivoire; Diagnosis; Helminths; Intestinal protozoa; Microscopy; Multiplex PCR; Persistent diarrhoea; Stool; Viruses

Indexed keywords

ALBENDAZOLE; IVERMECTIN; METRONIDAZOLE; NUCLEIC ACID; PRAZIQUANTEL;

ADOLESCENT; ADULT; ANTIGEN DETECTION; ARTICLE; BACTERIUM; CHILD; CONTROLLED STUDY; DIAGNOSTIC EQUIPMENT; DIAGNOSTIC PROCEDURE; DIARRHEA; ENTEROTOXIGENIC ESCHERICHIA COLI; FECES ANALYSIS; FEMALE; GIARDIA INTESTINALIS; GIARDIASIS; HELMINTH; HELMINTHIASIS; HUMAN; MAJOR CLINICAL STUDY; MALE; MICROORGANISM DETECTION; MICROSCOPY; MIDDLE AGED; MIXED INFECTION; MULTIPLEX POLYMERASE CHAIN REACTION; PARASITE; PARASITOSIS; PRIORITY JOURNAL; PROTOZOON; SCHISTOSOMIASIS; SCREENING; SHIGELLA; SPECIES IDENTIFICATION; STRONGYLOIDES STERCORALIS; STRONGYLOIDIASIS; VIRUS; AGED; ANIMAL; CASE CONTROL STUDY; COINFECTION; COTE D'IVOIRE; DIAGNOSTIC TEST; FECES; INFANT; ISOLATION AND PURIFICATION; MICROBIOLOGY; PARASITOLOGY; PRESCHOOL CHILD; PREVALENCE; PROCEDURES; VERY ELDERLY; VIROLOGY; YOUNG ADULT;

ESCHERICHIA COLI; GIARDIA INTESTINALIS; PROTOZOA; SHIGELLA; STRONGYLOIDES STERCORALIS; VERMES;

ADOLESCENT; ADULT; AGED; AGED, 80 AND OVER; ANIMALS; BACTERIA; CASE-CONTROL STUDIES; CHILD; CHILD, PRESCHOOL; COINFECTION; COTE D'IVOIRE; DIAGNOSTIC TESTS, ROUTINE; DIARRHEA; FECES; FEMALE; HUMANS; INFANT; MALE; MICROSCOPY; MIDDLE AGED; MULTIPLEX POLYMERASE CHAIN REACTION; PARASITES; PREVALENCE; VIRUSES; YOUNG ADULT;

EID: 84930819818     PISSN: 1198743X     EISSN: 14690691     Source Type: Journal    
DOI: 10.1016/j.cmi.2015.02.016     Document Type: Article

References (32)

1. Murray C.J.L., Vos T., Lozano R., Naghavi M., Flaxman A.D., Michaud C., et al. Disability-adjusted life years (DALYs) for 291 diseases and injuries in 21 regions, 1990-2010: a systematic analysis for the Global Burden of Disease Study 2010. Lancet 2012, 380:2197-2223.
2. Lozano R., Naghavi M., Foreman K., Lim S., Shibuya K., Aboyans V., et al. Global and regional mortality from 235 causes of death for 20 age groups in 1990 and 2010: a systematic analysis for the Global Burden of Disease Study 2010. Lancet 2012, 380:2095-2128.
3. Kotloff K.L., Nataro J.P., Blackwelder W.C., Nasrin D., Farag T.H., Panchalingam S., et al. Burden and aetiology of diarrhoeal disease in infants and young children in developing countries (the Global Enteric Multicenter Study, GEMS): a prospective, case-control study. Lancet 2013, 382:209-222.
4. Becker S.L., Vogt J., Knopp S., Panning M., Warhurst D.C., Polman K., et al. Persistent digestive disorders in the tropics: causative infectious pathogens and reference diagnostic tests. BMC Infect Dis 2013, 13:37.
5. DuPont H.L. Acute infectious diarrhea in immunocompetent adults. NEngl J Med 2014, 370:1532-1540.
6. Pawlowski S.W., Warren C.A., Guerrant R. Diagnosis and treatment of acute or persistent diarrhea. Gastroenterology 2009, 136:1874-1886.
7. Drancourt M. Multiplex testing of diarrhoea breaks down microbial barriers. Lancet Infect Dis 2014, 14:663-664.
8. Mengelle C., Mansuy J.M., Prere M.F., Grouteau E., Claudet I., Kamar N., et al. Simultaneous detection of gastrointestinal pathogens with a multiplex Luminex-based molecular assay in stool samples from diarrhoeic patients. Clin Microbiol Infect 2013, 19:E458-E465.
9. ® GPP) testing in the diagnosis of infectious gastroenteritis. Clin Microbiol Infect 2014, 20:O182-O187.
10. Gray J., Coupland L.J. The increasing application of multiplex nucleic acid detection tests to the diagnosis of syndromic infections. Epidemiol Infect 2014, 142:1-11.
11. Beckmann C., Heininger U., Marti H., Hirsch H.H. Gastrointestinal pathogens detected by multiplex nucleic acid amplification testing in stools of pediatric patients and patients returning from the tropics. Infection 2014, 42:961-970.
12. Zboromyrska Y., Hurtado J.C., Salvador P., Alvarez-Martínez M.J., Valls M.E., Mas J., et al. Aetiology of traveller's diarrhoea: evaluation of a multiplex PCR tool to detect different enteropathogens. Clin Microbiol Infect 2014, 20:O753-O759.
13. Knopp S., Mgeni A.F., Khamis I.S., Steinmann P., Stothard J.R., Rollinson D., et al. Diagnosis of soil-transmitted helminths in the era of preventive chemotherapy: effect of multiple stool sampling and use of different diagnostic techniques. PLoS Negl Trop Dis 2008, 2:e331.
14. Chappuis F., Alirol E., d'Acremont V., Bottieau E., Yansouni C.P. Rapid diagnostic tests for non-malarial febrile illness in the tropics. Clin Microbiol Infect 2013, 19:422-431.
15. Yansouni C.P., Bottieau E., Chappuis F., Phoba M.F., Lunguya O., Ifeka B.B., et al. Rapid diagnostic tests for neurological infections in central Africa. Lancet Infect Dis 2013, 13:546-558.
16. World Health Organization (WHO) Diarrhoeal disease August 2009, Fact sheet no. 330, Geneva.
17. Kahlau P., Malecki M., Schildgen V., Schulz C., Winterfeld I., Messler S., et al. Utility of two novel multiplexing assays for the detection of gastrointestinal pathogens-a first experience. SpringerPlus 2013, 2:106.
18. Cohen J. Acoefficient of agreement for nominal scales. Educ Psychol Meas 1960, 20:213-220.
19. Diamond L.S., Clark C.G. Aredescription of Entamoeba histolytica Schaudinn, 1903 (Emended Walker, 1911) separating it from Entamoeba dispar Brumpt, 1925. JEukaryot Microbiol 1993, 40:340-344.
20. Liu J., Kabir F., Manneh J., Lertsethtakarn P., Begum S., Gratz J., et al. Development and assessment of molecular diagnostic tests for 15 enteropathogens causing childhood diarrhoea: a multicentre study. Lancet Infect Dis 2014, 14:716-724.
21. Claas E.C., Burnham C.A., Mazzulli T., Templeton K., Topin F. Performance of the xTAG gastrointestinal pathogen panel, a multiplex molecular assay for simultaneous detection of bacterial, viral, and parasitic causes of infectious gastroenteritis. JMicrobiol Biotechnol 2013, 23:1041-1045.
22. Halligan E., Edgeworth J., Bisnauthsing K., Bible J., Cliff P., Aarons E., et al. Multiplex molecular testing for management of infectious gastroenteritis in a hospital setting: a comparative diagnostic and clinical utility study. Clin Microbiol Infect 2014, 20:O460-O467.
23. Navidad J.F., Griswold D.J., Gradus M.S., Bhattacharyya S. Evaluation of Luminex xTAG gastrointestinal pathogen analyte-specific reagents for high-throughput, simultaneous detection of bacteria, viruses, and parasites of clinical and public health importance. JClin Microbiol 2013, 51:3018-3024.
24. Khare R., Espy M.J., Cebelinski E., Boxrud D., Sloan L.M., Cunningham S.A., et al. Comparative evaluation of two commercial multiplex panels for detection of gastrointestinal pathogens by use of clinical stool specimens. JClin Microbiol 2014, 52:3667-3673.
25. Patel A., Navidad J., Bhattacharyya S. Site-specific clinical evaluation of the Luminex xTAG gastrointestinal pathogen panel for detection of infectious gastroenteritis in fecal specimens. JClin Microbiol 2014, 52:3068-3071.
26. Coulibaly J.T., Fürst T., Silué K.D., Knopp S., Hauri D., Ouattara M., et al. Intestinal parasitic infections in schoolchildren in different settings of Côte d'Ivoire: effect of diagnostic approach and implications for control. Parasit Vectors 2012, 5:135.
27. Kinross J.M., Darzi A.W., Nicholson J.K. Gut microbiome-host interactions in health and disease. Genome Med 2011, 3:14.
28. Hürlimann E., Houngbedji C.A., N'Dri P.B., Bänninger D., Coulibaly J.T., Yap P., et al. Effect of deworming on school-aged children's physical fitness, cognition and clinical parameters in a malaria-helminth co-endemic area of Côte d'Ivoire. BMC Infect Dis 2014, 14:411.
29. Abba K., Sinfield R., Hart C.A., Garner P. Pathogens associated with persistent diarrhoea in children in low and middle income countries: systematic review. BMC Infect Dis 2009, 9:88.
30. Luminex Corporation xMAP® Cookbook. A collection of methods and protocols for developing multiplex assays with xMAP technology 2014, Available at:, [accessed 05.02.15]. http://info2.luminexcorp.com/download-the-xmap-cookbook, 2nd ed.
31. Verweij J.J. Application of PCR-based methods for diagnosis of intestinal parasitic infections in the clinical laboratory. Parasitology 2014, 141:1863-1872.
32. Utzinger J., Booth M., N'Goran E.K., Müller I., Tanner M., Lengeler C. Relative contribution of day-to-day and intra-specimen variation in faecal egg counts of Schistosoma mansoni before and after treatment with praziquantel. Parasitology 2001, 122:537-544.