Comparative evaluation of two commercial multiplex panels for detection of gastrointestinal pathogens by use of clinical stool specimens

Journal of Clinical Microbiology

Volumn 52, Issue 10, 2014, Pages 3667-3673

  Khare, Reeti ^a   Espy, Mark J ^a   Cebelinski, Elizabeth ^b   Boxrud, David ^b   Sloan, Lynne M ^a   Cunningham, Scott A ^a   Pritt, Bobbi S ^a   Patel, Robin ^a   Binnicker, Matthew J ^a  

^a MAYO CLINIC   (United States)

^b MINNESOTA DEPARTMENT OF HEALTH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AEROMONAS; ARTICLE; CLOSTRIDIUM DIFFICILE; COMPARATIVE STUDY; CONTROLLED STUDY; DIAGNOSTIC KIT; FECES ANALYSIS; GASTROINTESTINAL TRACT; IMMUNOASSAY; MICROORGANISM DETECTION; MICROSCOPY; NONHUMAN; REAL TIME POLYMERASE CHAIN REACTION; SAPOVIRUS; VIRAL GENETICS; VIRUS DETECTION; YERSINIA ENTEROCOLITICA; ANIMAL; BACTERIAL INFECTIONS; BACTERIUM; CLASSIFICATION; EVALUATION STUDY; FECES; GASTROINTESTINAL DISEASES; GENETICS; HUMAN; INTESTINAL DISEASES, PARASITIC; ISOLATION AND PURIFICATION; MICROBIOLOGY; MOLECULAR DIAGNOSIS; PARASITE; PARASITOLOGY; PROCEDURES; PROSPECTIVE STUDY; SENSITIVITY AND SPECIFICITY; VIROLOGY; VIRUS; VIRUS DISEASES;

ANIMALS; BACTERIA; BACTERIAL INFECTIONS; FECES; GASTROINTESTINAL DISEASES; HUMANS; INTESTINAL DISEASES, PARASITIC; MOLECULAR DIAGNOSTIC TECHNIQUES; PARASITES; PROSPECTIVE STUDIES; SENSITIVITY AND SPECIFICITY; VIRUS DISEASES; VIRUSES;

EID: 84907173551     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.01637-14     Document Type: Article

References (24)

1. Petri WA, Jr, Miller M, Binder HJ, Levine MM, Dillingham R, Guerrant RL. 2008. Enteric infections, diarrhea, and their impact on function and development. J. Clin. Invest. 118:1277-1290. http://dx.doi.org/10.1172/JCI34005.
2. Kotloff KL, Nataro JP, Blackwelder WC, Nasrin D, Farag TH, Panchalingam S, Wu Y, Sow SO, Sur D, Breiman RF, Faruque AS, Zaidi AK, Saha D, Alonso PL, Tamboura B, Sanogo D, Onwuchekwa U, Manna B, Ramamurthy T, Kanungo S, Ochieng JB, Omore R, Oundo JO, Hossain A, Das SK, Ahmed S, Qureshi S, Quadri F, Adegbola RA, Antonio M, Hossain MJ, Akinsola A, Mandomando I, Nhampossa T, Acacio S, Biswas K, OReilly CE, Mintz ED, Berkeley LY, Muhsen K, Sommerfelt H, Robins-Browne RM, Levine MM. 2013. Burden and aetiology of diarrhoeal disease in infants and young children in developing countries (the Global Enteric Multicenter Study, GEMS): a prospective, case-control study. Lancet 382:209-222. http://dx.doi.org/10.1016/S0140-6736(13)60844-2.
3. Esposito DH, Holman RC, Haberling DL, Tate JE, Podewils LJ, Glass RI, Parashar U. 2011. Baseline estimates of diarrhea-associated mortality among United States children before rotavirus vaccine introduction. Pediatr. Infect. Dis. J. 30:942-947. http://dx.doi.org/10.1097/INF .0b013e3182254d19.
4. Hennessy TW, Marcus R, Deneen V, Reddy S, Vugia D, Townes J, Bardsley M, Swerdlow D, Angulo FJ. 2004. Survey of physician diagnostic practices for patients with acute diarrhea: clinical and public health implications. Clin. Infect. Dis. 38(Suppl 3):S203-S211. http://dx.doi.org/10.1086/381588.
5. Scallan E, Hoekstra RM, Angulo FJ, Tauxe RV, Widdowson MA, Roy SL, Jones JL, Griffin PM. 2011. Foodborne illness acquired in the United States-major pathogens. Emerg. Infect. Dis. 17:7-15. http://dx.doi.org/10.3201/eid1701.09-1101p1.
6. Schlenker C, Surawicz CM. 2009. Emerging infections of the gastrointestinal tract. Best Pract Res. Clin. Gastroenterol. 23:89-99. http://dx.doi.org/10.1016/j.bpg.2008.11.014.
7. Malecki M, Schildgen V, Kamm M, Mattner F, Schildgen O. 2012. Rapid screening method for multiple gastroenteric pathogens also detects novel enterohemorrhagic Escherichia coli O104:H4. Am. J. Infect. Control 40: 82-83. http://dx.doi.org/10.1016/j.ajic.2011.07.019.
8. Hatchette TF, Farina D. 2011. Infectious diarrhea: when to test and when to treat. CMAJ 183:339-344. http://dx.doi.org/10.1503/cmaj.091495.
9. Dunbar SA. 2013. Molecular revolution entering GI diagnostic testing. MLO Med. Lab. Obs. 45:28.
10. Cunningham SA, Sloan LM, Nyre LM, Vetter EA, Mandrekar J, Patel R. 2010. Three-hour molecular detection of Campylobacter, Salmonella, Yersinia, and Shigella species in feces with accuracy as high as that of culture. J. Clin. Microbiol. 48:2929-2933. http://dx.doi.org/10.1128/JCM .00339-10.
11. Karre T, Sloan L, Patel R, Mandrekar J, Rosenblatt J. 2011. Comparison of two commercial molecular assays to a laboratory-developed molecular assay for diagnosis of Clostridium difficile infection. J. Clin. Microbiol. 49:725-727. http://dx.doi.org/10.1128/JCM.01028-10.
12. Buckwalter SP, Teo R, Espy MJ, Sloan LM, Smith TF, Pritt BS. 2012. Real-time qualitative PCR for 57 human adenovirus types from multiple specimen sources. J. Clin. Microbiol. 50:766-771. http://dx.doi.org/10 .1128/JCM.05629-11.
13. Visvesvara GS, Moura H, Kovacs-Nace E, Wallace S, Eberhard ML. 1997. Uniform staining of Cyclospora oocysts in fecal smears by a modified safranin technique with microwave heating. J. Clin. Microbiol. 35:730-733.
14. Trujillo AA, McCaustland KA, Zheng DP, Hadley LA, Vaughn G, Adams SM, Ando T, Glass RI, Monroe SS. 2006. Use of TaqMan realtime reverse transcription-PCR for rapid detection, quantification, and typing of norovirus. J. Clin. Microbiol. 44:1405-1412. http://dx.doi.org/10.1128/JCM.44.4.1405-1412.2006.
15. Oka T, Katayama K, Hansman GS, Kageyama T, Ogawa S, Wu FT, White PA, Takeda N. 2006. Detection of human sapovirus by real-time reverse transcription-polymerase chain reaction. J. Med. Virol. 78:1347-1353. http://dx.doi.org/10.1002/jmv.20699.
16. Grant L, Vinje J, Parashar U, Watt J, Reid R, Weatherholtz R, Santosham M, Gentsch J, OBrien K. 2012. Epidemiologic and clinical features of other enteric viruses associated with acute gastroenteritis in American Indian infants. J. Pediatr. 161:110-115, e111. http://dx.doi.org/10.1016/j.jpeds.2011.12.046.
17. Freeman MM, Kerin T, Hull J, McCaustland K, Gentsch J. 2008. Enhancement of detection and quantification of rotavirus in stool using a modified real-time RT-PCR assay. J. Med. Virol. 80:1489-1496. http://dx .doi.org/10.1002/jmv.21228.
18. Heim A, Ebnet C, Harste G, Pring-Akerblom P. 2003. Rapid and quantitative detection of human adenovirus DNA by real-time PCR. J. Med. Virol. 70:228-239. http://dx.doi.org/10.1002/jmv.10382.
19. Stefanoff P, Rogalska J, Czech M, Staszewska E, Rosinska M. 2013. Antibacterial prescriptions for acute gastrointestinal infections: uncovering the iceberg. Epidemiol. Infect. 141:859-867. http://dx.doi.org/10 .1017/S0950268812001173.
20. Kotwani A, Chaudhury RR, Holloway K. 2012. Antibiotic-prescribing practices of primary care prescribers for acute diarrhea in New Delhi, India. Value Health 15:S116-S119. http://dx.doi.org/10.1016/j.jval.2011 .11.008.
21. Ekwochi U, Chinawa JM, Obi I, Obu HA, Agwu S. 2013. Use and/or misuse of antibiotics in management of diarrhea among children in Enugu, Southeast Nigeria. J. Trop. Pediatr. 59:314-316. http://dx.doi.org/10.1093/tropej/fmt016.
22. Navidad JF, Griswold DJ, Gradus MS, Bhattacharyya S. 2013. Evaluation of Luminex xTAG gastrointestinal pathogen analyte-specific reagents for high-throughput, simultaneous detection of bacteria, viruses, and parasites of clinical and public health importance. J. Clin. Microbiol. 51: 3018-3024. http://dx.doi.org/10.1128/JCM.00896-13.
23. Claas EC, Burnham CA, Mazzulli T, Templeton K, Topin F. 2013. Performance of the xTAG(R) gastrointestinal pathogen panel, a multiplex molecular assay for simultaneous detection of bacterial, viral, and parasitic causes of infectious gastroenteritis. J. Microbiol. Biotechnol. 23: 1041-1045. http://dx.doi.org/10.4014/jmb.1212.12042.
24. Lindsay B, Ramamurthy T, Sen Gupta S, Takeda Y, Rajendran K, Nair GB, Stine OC. 2011. Diarrheagenic pathogens in polymicrobial infections. Emerg. Infect. Dis. 17:606-611. http://dx.doi.org/10.3201/eid1704 .100939