Interleukin-1α activation and localization in lipopolysaccharide-stimulated human monocytes and macrophages

Journal of Immunological Methods

Volumn 422, Issue , 2015, Pages 59-71

  Carlsen, Thomas Gelsing ^a   Kjærsgaard, Pernille ^a   Jørgensen, Trine Lykke ^a   Foldbjerg, Rasmus ^a   Nielsen, Mads Lausen ^a   Poulsen, Thomas Bouet Guldbæk ^a   Zabieglo, Katarzyna ^b   Christiansen, Gunna ^b   Birkelund, Svend ^a,b  

^a Department of Health Science and Technology ^*  (Denmark)

^b Loke Diagnostics   (Denmark)

Author keywords

Flow cytometry; IL 1 ; Intranuclear staining; Monoclonal antibody; Monocytes

Indexed keywords

CD68 ANTIGEN; FLUORESCEIN ISOTHIOCYANATE; INTERLEUKIN 1ALPHA; LIPOPOLYSACCHARIDE; MESSENGER RNA; MONOCLONAL ANTIBODY; CD68 ANTIGEN, HUMAN; DIFFERENTIATION ANTIGEN; FLUORESCENT DYE; IL1A PROTEIN, HUMAN; LEUKOCYTE ANTIGEN;

ARTICLE; CONFOCAL MICROSCOPY; CYTOKINE PRODUCTION; ENZYME LINKED IMMUNOSORBENT ASSAY; FLOW CYTOMETRY; HUMAN; HUMAN CELL; IN VITRO STUDY; MACROPHAGE; MONOCYTE; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN CLEAVAGE; RNA SEQUENCE; ANIMAL; BLOOD; ENZYME ACTIVATION; GENETICS; HELA CELL LINE; IMMUNOLOGY; INFLAMMATION; METABOLISM; MOUSE; PROCEDURES; SEQUENCE ANALYSIS; TUMOR CELL LINE;

ANIMALS; ANTIBODIES, MONOCLONAL; ANTIGENS, CD; ANTIGENS, DIFFERENTIATION, MYELOMONOCYTIC; BASE SEQUENCE; CELL LINE, TUMOR; ENZYME ACTIVATION; FLOW CYTOMETRY; FLUORESCENT DYES; HELA CELLS; HUMANS; INFLAMMATION; INTERLEUKIN-1ALPHA; LIPOPOLYSACCHARIDES; MACROPHAGES; MICE; MONOCYTES; RNA, MESSENGER; SEQUENCE ANALYSIS, RNA;

EID: 84930758672     PISSN: 00221759     EISSN: 18727905     Source Type: Journal    
DOI: 10.1016/j.jim.2015.03.025     Document Type: Article

References (67)

1. Aarden L. Revised nomenclature for antigen-nonspecific T cell proliferation and helper factors. J. Immunol. 1979, 123:2928.
2. Afonina I.S., Tynan G.A., Logue S.E., Cullen S.P., Bots M., Lüthi A.U., et al. Granzyme B-dependent proteolysis acts as a switch to enhance the proinflammatory activity of IL-1α. Mol Cell. 2011, 44:265-278.
3. Alnemri E.S., Livingston D.J., Nicholson D.W., Salvesen G., Thornberry N.A., Wong W.W., Yuan J. Human ICE/CED-3 protease nomenclature. Cell 1996, 87:171.
4. Arend W.P., Massoni R.J. Characteristics of bacterial lipopolysaccharide induction of interleukin 1 synthesis and secretion by human monocytes. Clin. Exp. Immunol. 1986, 64:656.
5. Auron P.E., Webb A.C., Rosenwasser L.J., Mucci S.F., Rich A., Wolff S.M., Dinarello C.A. Nucleotide sequence of human monocyte interleukin 1 precursor cDNA. Proc. Natl. Acad. Sci. U. S. A. 1984, 81:7907.
6. Axelsen T.V., Holm A., Birkelund S., Christiansen G., Ploug M., Holm I.E. Specific recognition of the C-terminal end of Aβ42 by a high affinity monoclonal antibody. Mol. Immunol. 2009, 46:2267. 10.1016/j.molimm.2009.04.007.
7. Beasley D., Cooper A.L. Constitutive expression of interleukin-1alpha precursor promotes human vascular smooth muscle cell proliferation. Am. J. Physiol. 1999, 276:H901.
8. Beranek J.T. CD68 is not a macrophage-specific antigen. Ann. Rheum. Dis. 2005, 64:342. (author reply 343-4).
9. Berg K.E., Ljungcrantz I., Andersson L., Bryngelsson C., Hedblad B., Fredrikson G.N., Nilsson J., Bjorkbacka H. Elevated CD14++CD16-monocytes predict cardiovascular events. Circ. Cardiovasc. Genet. 2012, 5:122. 10.1161/CIRCGENETICS.111.960385.
10. Birkelund S., Lundemose A.G., Christiansen G. Chemical cross-linking of Chlamydia trachomatis. Infect. Immun. 1988, 56:654.
11. Cerretti D.P., Kozlosky C.J., Mosley B., Nelson N., Van Ness K., Greenstreet T.A., March C.J., Kronheim S.R., Druck T., Cannizzaro L.A. Molecular cloning of the interleukin-1 beta converting enzyme. Science 1992, 256:97.
12. Contassot E., Beer H.D., French L.E. Interleukin-1, inflammasomes, autoinflammation and the skin. Swiss Med. Wkly. 2012, 10.4414/smw.2012.13590.
13. Di Paolo N.C., Shayakhmetov D.M. Interleukin-1 Receptor 2 Keeps the Lid on Interleukin-1α. Immuni 2013, 38(2):203-205. 10.1016/j.immuni.2013.02.001.
14. Dinarello C.A. Immunological and inflammatory functions of the interleukin-1 family. Annu. Rev. Immunol. 2009, 27:519. 10.1146/annurev.immunol.021908.132612.
15. Dinarello C.A. Interleukin-1 in the pathogenesis and treatment of inflammatory diseases. Blood 2011, 117:3720. 10.1182/blood-2010-07-273417.
16. Dinarello C.A., Goldin N.P., Wolff S.M. Demonstration and characterization of two distinct human leukocytic pyrogens. J. Exp. Med. 1974, 139:1369.
17. Drasbek M., Nielsen P.K., Persson K., Birkelund S., Christiansen G. Immune response to Mycoplasma pneumonia P1 and P116 in patients with atypical pneumonia analyzed by ELISA. BMC Microbiol. 2004, 4:7. 10.1186/1471-2180-4-7.
18. Farrar W.L., Mizel S.B., Farrar J.J. Participation of lymphocyte activating factor (Interleukin 1) in the induction of cytotoxic T cell responses. J. Immunol. 1980, 124:1371.
19. Fettelschoss A., Kistowska M., LeibundGut-Landmann S., Beer H.-D., Johansen P., Senti G., Contassot E., Bachmann M.F., French L.E., Oxenius A., Kündig T.M. Inflammasome activation and IL-1β target IL-1α for secretion as opposed to surface expression. Proc. Natl. Acad. Sci. 2011, 108:18055. 10.1073/pnas.1109176108.
20. Fettelschoss A., Kistowska M., LeibundGut-Landmann S., Beer H.-D., Johansen P., Senti G., Contassot E., Bachmann M.F., French L.E., Oxenius A., Kündig T.M. Inflammasome activation and IL-1β target IL-1α for secretion as opposed to surface expression. Proc. Natl. Acad. Sci. 2011, 108:18055. 10.1073/pnas.1109176108.
21. Franken K.L.M.C., Hiemstra H.S., van Meijgaarden K.E., Subronto Y., den Hartigh J., Ottenhoff T.H.M., Drijfhout J.W. Purification of His-tagged proteins by immobilized chelate affinity chromatography: the benefits from the use of organic solvent. Protein Expr. Purif. 2000, 18:95. 10.1006/prep.1999.1162.
22. Gabay C., Lamacchia C., Palmer G. IL-1 pathways in inflammation and human diseases. Nat. Rev. Rheumatol. 2010, 6:232. 10.1038/nrrheum.2010.4.
23. Gery I., Waksman B.H. Potentiation of the T-lymphocyte response to mitogens. II. The cellular source of potentiating mediator(s). J. Exp. Med. 1972, 136:143.
24. Gery I., Davies P., Derr J., Krett N., Barranger J.A. Relationship between production and release of lymphocyte-activating factor (interleukin 1) by murine macrophages. 1. Effects of various agents. Cell. Immunol. 1981, 64:293.
25. Goldbach-Mansky R. Blocking interleukin-1 in rheumatic diseases. Ann. N. Y. Acad. Sci. 2009, 1182:111. 10.1111/j.1749-6632.2009.05159.x.
26. Groß O., Yazdi A.S., Thomas C.J., Masin M., Heinz L.X., Guarda G., Quadroni M., Drexler S.K., Tschopp J. Inflammasome activators induce interleukin-1a secretion via distinct pathways with differential requirement for the protease function of caspase-1. Immunity 2012, 36:388. 10.1016/j.immuni.2012.01.018.
27. Guenin S., Mauriat M., Pelloux J., Van Wuytswinkel O., Bellini C., Gutierrez L. Normalization of qRT-PCR data: the necessity of adopting a systematic, experimental conditions-specific, validation of references. J. Exp. Bot. 2009, 60:487. 10.1093/jxb/ern305.
28. Heguy A., Baldari C.T., Macchia G., Telford J.L., Melli M. Amino acids conserved in interleukin-1 receptors (IL-1Rs) and the Drosophila toll protein are essential for IL-1R signal transduction. J. Biol. Chem. 1992, 267:2605.
29. Hu B., Wang S., Zhang Y., Feghali C.A., Dingman J.R., Wright T.M. A nuclear target for interleukin-1alpha: interaction with the growth suppressor necdin modulates proliferation and collagen expression. Proc. Natl. Acad. Sci. U. S. A. 2003, 100:10008. 10.1073/pnas.1737765100.
30. Iwasaki A., Medzhitov R. Regulation of adaptive immunity by the innate immune system. Science 2010, 327:291. 10.1126/science.1183021.
31. Johnsen A.K., Plenge R.M., Butty V., Campbell C., Dieguez Gonzalez R., Gomez Reino J.J., Shadick N., Weinblatt M., Gonzalez A., Gregersen P.K., Benoist C., Mathis D. A broad analysis of IL1 polymorphism and rheumatoid arthritis. Arthritis Rheum. 2008, 58:1947. 10.1002/art.23592.
32. Kim B., Lee Y., Kim E., Kwak A., Ryoo S., Bae S.H., Azam T., Kim S., Dinarello C.A. The interleukin-1α Precursor is biologically active and is likely a key alarmin in the IL-1 family of cytokines. Front. Immunol. 2013, 4:391. 10.3389/fimmu.2013.00391.
33. Kirschning C.J., Wesche H., Merrill Ayres T., Rothe M. Human toll-like receptor 2 confers responsiveness to bacterial lipopolysaccharide. J. Exp. Med. 1998, 188:2091.
34. Kobayashi Y., Yamamoto K., Saido T., Kawasaki H., Oppenheim J.J., Matsushima K. Identification of calcium-activated neutral protease as a processing enzyme of human interleukin 1 alpha. Proc. Natl. Acad. Sci. U. S. A. 1990, 87:5548.
35. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227:680.
36. Lamacchia C., Rodriguez E., Palmer G., Gabay C. Endogenous IL-1α is a chromatin-associated protein in mouse macrophages. Cytokine 2013, 63:135. 10.1016/j.cyto.2013.04.010.
37. Lea W.-I., Lee Y.H. The associations between interleukin-1 polymorphisms and susceptibility to ankylosing spondylitis: a meta-analysis. Joint Bone Spine 2012, 79:370. 10.1016/j.jbspin.2011.06.010.
38. Lepe-Zuniga J.L., Gery I. Production of intra- and extracellular interleukin-1 (IL-1) by human monocytes. Clin. Immunol. Immunopathol. 1984, 31:222.
39. Lomedico P.T., Gubler U., Hellmann C.P., Dukovich M., Giri J.G., Pan Y.C., Collier K., Semionow R., Chua A.O., Mizel S.B. Cloning and expression of murine interleukin-1 cDNA in Escherichia coli. Nature 1984, 312:458.
40. Luheshi N.M., Rothwell N.J., Brough D. The dynamics and mechanisms of interleukin-1α and β nuclear import. Traffic 2008, 10:16. 10.1111/j.1600-0854.2008.00840.x.
41. Maeshima N., Fernandez R.C. Recognition of lipid A variants by the TLR4-MD-2 receptor complex. Front. Cell. Infect. Microbiol. 2013, 3:3. 10.3389/fcimb.2013.00003.
42. Maksymowych W.P., Rahman P., Reeve J.P., Gladman D.D., Peddle L., Inman R.D. Association of the IL1 gene cluster with susceptibility to ankylosing spondylitis: an analysis of three Canadian populations. Arthritis Rheum. 2006, 54:974. 10.1002/art.21642.
43. Mann C.L.A., Davies M.B., Stevenson V.L., Leary S.M., Boggild M.D., Ko Ko C., Jones P.W., Fryer A.A., Strange R.C., Thompson A.J., Hawkins C.P. Interleukin 1 genotypes in multiple sclerosis and relationship to disease severity. J. Neuroimmunol. 2002, 129:197.
44. March C.J., Mosley B., Larsen A., Cerretti D.P., Braedt G., Price V., Gillis S., Henney C.S., Kronheim S.R., Grabstein K. Cloning, sequence and expression of two distinct human interleukin-1 complementary DNAs. Nature 1985, 315:641.
45. Micklem K., Rigney E., Cordell J., Simmons D., Stross P., Turley H., Seed B., Mason D. A human macrophage-associated antigen (CD68) detected by six different monoclonal antibodies. Br. J. Haematol. 1989, 73:6.
46. Monnet D., Kadi A., Izac B., Lebrun N., Letourneur F., Zinovieva E., Said-Nahal R., Chiocchia G., Breban M. Association between the IL-1 family gene cluster and spondyloarthritis. Ann. Rheum. Dis. 2012, 71:885. 10.1136/annrheumdis-2011-200439.
47. Mora M., Carinci V., Bensi G., Raugei G., Buonamassa D.T., Melli M. Differential expression of the human IL-1 alpha and beta genes. Prog. Clin. Biol. Res. 1990, 349:205.
48. Needleman S.B., Wunsch C.D. A general method applicable to the search for similarities in the amino acid sequence of two proteins. J. Mol. Biol. 1970, 48:443.
49. Newton R.C. Human monocyte production of interleukin-1: parameters of the induction of interleukin-1 secretion by lipopolysaccharides. J. Leukoc. Biol. 1986, 39:299.
50. O'Neill L.A.J. The interleukin-1 receptor/Toll-like receptor superfamily: 10years of progress. Immunol. Rev. 2008, 226:10. 10.1111/j.1600-065X.2008.00701.x.
51. Poltorak A. Defective LPS signaling in C3H/HeJ and C57BL/10ScCr mice: mutations in Tlr4 gene. Science 1998, 282:2085. 10.1126/science.282.5396.2085.
52. Ross R., Grimmel J., Goedicke S., Möbus A.M., Bulau A.-M., Bufler P., Ali S., Martin M.U. Analysis of nuclear localization of interleukin-1 family cytokines by flow cytometry. J. Immunol. Methods 2013, 387:219. 10.1016/j.jim.2012.10.017.
53. Schulte T., Schöls L., Müller T., Woitalla D., Berger K., Krüger R. Polymorphisms in the interleukin-1 alpha and beta genes and the risk for Parkinson's disease. Neurosci. Lett. 2002, 326:70.
54. Schultz K., Murthy V., Tatro J.B., Beasley D. Endogenous interleukin-1 promotes a proliferative and proinflammatory phenotype in human vascular smooth muscle cells. Am. J. Physiol. Heart Circ. Physiol. 2007, 292:H2927. 10.1152/ajpheart.00700.2006.
55. Shaw A.C., Christiansen G., Roepstorff P., Birkelund S. Genetic differences in the Chlamydia trachomatis tryptophan synthase alpha-subunit can explain variations in serovar pathogenesis. Microbes Infect. 2000, 2:581.
56. Sims A.-M., Timms A.E., Bruges-Armas J., Burgos-Vargas R., Chou C.T., Doan T., Dowling A., Fialho R.N., Gergely P., Gladman D.D., Inman R., Kauppi M., Kaarela K., Laiho K., Maksymowych W., Pointon J.J., Rahman P., Reveille J.D., Sorrentino R., Tuomilehto J., Vargas-Alarcon G., Wordsworth B.P., Xu H., Brown M.A., on behalf of the International Genetics of Ankylosing Spondylitis Prospective meta-analysis of interleukin 1 gene complex polymorphisms confirms associations with ankylosing spondylitis. Ann. Rheum. Dis. 2007, 67:1305. 10.1136/ard.2007.081364.
57. Stevenson F.T., Bursten S.L., Fanton C., Locksley R.M., Lovett D.H. The 31-kDa precursor of interleukin lα is myristoylated on specific lysines within the 16-kDa N-terminal propiece. Proc. Natl. Acad. Sci. U. S. A. 1993, 90:7245.
58. Stevenson F.T., Turck J., Locksley R.M., Lovett D.H. The N-terminal propiece of interleukin 1 alpha is a transforming nuclear oncoprotein. Proc. Natl. Acad. Sci. U. S. A. 1997, 94:508.
59. Thornberry N.A., Bull H.G., Calaycay J.R., Chapman K.T., Howard A.D., Kostura M.J., Miller D.K., Molineaux S.M., Weidner J.R., Aunins J. A novel heterodimeric cysteine protease is required for interleukin-1 beta processing in monocytes. Nature 1992, 356:768. 10.1038/356768a0.
60. Ulevitch R.J., Tobias P.S. Recognition of endotoxin by cells leading to transmembrane signaling. Curr. Opin. Immunol. 1994, 6:125.
61. Wang D., Zhang S., Li L., Liu X., Mei K., Wang X. Structural insights into the assembly and activation of IL-1β with its receptors. Nat. Immunol. 2010, 11:905. 10.1038/ni.1925.
62. Werman A., Werman-Venkert R., White R., Lee J.-K., Werman B., Krelin Y., Voronov E., Dinarello C.A., Apte R.N. The precursor form of IL-1alpha is an intracrine proinflammatory activator of transcription. Proc. Natl. Acad. Sci. U. S. A. 2004, 101:2434.
63. Wessendorf J.H., Garfinkel S., Zhan X., Brown S., Maciag T. Identification of a nuclear localization sequence within the structure of the human interleukin-1 alpha precursor. J. Biol. Chem. 1993, 268:22100.
64. Yang R.B., Mark M.R., Gray A., Huang A., Xie M.H., Zhang M., Goddard A., Wood W.I., Gurney A.L., Godowski P.J. Toll-like receptor-2 mediates lipopolysaccharide-induced cellular signalling. Nature 1998, 395:284. 10.1038/26239.
65. Yazdi A.S., Drexler S.K. Regulation of interleukin 1 secretion by inflammasomes. Ann. Rheum. Dis. 2013, 72:ii96. 10.1136/annrheumdis-2012-202252.
66. Zhang A.-Q., Pan W., Gao J.-W., Yue C.-L., Zeng L., Gu W., Jiang J.-X. Associations between interleukin-1 gene polymorphisms and sepsis risk: a meta-analysis. BMC Med. Genet. 2014, 15:8. 10.1186/1471-2350-15-8.
67. Zheng Y., Humphry M., Maguire J.J., Bennett M.R., Clarke M.C.H. Intracellular interleukin-1 receptor 2 Binding prevents cleavage and activity of interleukin-1a, controlling necrosis-induced sterile inflammation. Immunity 2013, 38:285. 10.1016/j.immuni.2013.01.008.