Ribotag is a flexible tool for measuring the translational state of targeted cells in heterogeneous cell cultures

BioTechniques

Volumn 58, Issue 6, 2015, Pages 308-317

  Lesiak, Adam J ^a   Brodsky, Matthew ^a   Neumaier, John F ^a  

^a UNIVERSITY OF WASHINGTON   (United States)

Author keywords

Cell type specific gene expression; RiboTag; Translational profiling; TRAP. primary neuronal cultures

Indexed keywords

COMPLEMENTARY DNA; DIMETHYL SULFOXIDE; ENHANCED GREEN FLUORESCENT PROTEIN; HEMAGGLUTININ; SHORT HAIRPIN RNA; SMALL INTERFERING RNA; RNA;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; CELL SPECIFICITY; CELL VIABILITY; CONTROLLED STUDY; GENE AMPLIFICATION; GENE EXPRESSION; GENE SILENCING; GENETIC PROCEDURES; GENETIC TRANSFECTION; HOUSEKEEPING GENE; IMAGING; IMMUNOPRECIPITATION; LASER CAPTURE MICRODISSECTION; MOUSE; NERVE CELL CULTURE; NERVE CELL IMAGING; NONHUMAN; PLASMID; POLYSOME; REVERSE TRANSCRIPTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RIBOSOME SUBUNIT; RIBOTAG; RNA ISOLATION; RNA PURIFICATION; RNA TRANSLATION; SENSITIVITY ANALYSIS; ANIMAL; C57BL MOUSE; CELL CULTURE; COCULTURE; CYTOLOGY; GENE EXPRESSION REGULATION; GENETICS; GLIA; ISOLATION AND PURIFICATION; METABOLISM; NERVE CELL; PROTEIN SYNTHESIS; TRANSGENIC MOUSE;

MUS MUSCULUS;

ANIMALS; CELLS, CULTURED; COCULTURE TECHNIQUES; GENE EXPRESSION REGULATION; MICE, INBRED C57BL; MICE, TRANSGENIC; NEUROGLIA; NEURONS; PLASMIDS; POLYRIBOSOMES; PROTEIN BIOSYNTHESIS; REVERSE TRANSCRIPTION; RNA; TRANSFECTION;

EID: 84930689170     PISSN: 07366205     EISSN: 19409818     Source Type: Journal    
DOI: 10.2144/000114299     Document Type: Article

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