Fast and reliable production, purification and characterization of heat-stable, bifunctional enzyme chimeras

AMB Express

Volumn 5, Issue 1, 2015, Pages

  Neddersen, Mara ^a   Elleuche, Skander ^a  

^a HAMBURG UNIVERSITY OF TECHNOLOGY   (Germany)

Author keywords

Bifunctional fusion enzyme; Cellulases; Extremophiles; Synergism; Thermozymes

Indexed keywords

BETA GLUCOSIDASE; CELLOBIOSE; GLUCAN SYNTHASE; HYBRID PROTEIN; OLIGOSACCHARIDE;

AFFINITY CHROMATOGRAPHY; ARTICLE; CHIMERA; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME KINETICS; ENZYME PURIFICATION; ENZYME SYNTHESIS; ESCHERICHIA COLI; FUSION GENE; GENE EXPRESSION; GENE SEQUENCE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; NONHUMAN; OPEN READING FRAME; PH; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; SITE DIRECTED MUTAGENESIS; TEMPERATURE; THERMOSTABILITY; WESTERN BLOTTING; ZYMOGRAPHY;

EID: 84930651395     PISSN: None     EISSN: 21910855     Source Type: Journal    
DOI: 10.1186/s13568-015-0122-7     Document Type: Article

References (34)

1. Adlakha N, Sawant S, Anil A, Lali A, Yazdani SS (2012) Specific fusion of β-1,4-endoglucanase and β-1,4-glucosidase enhances cellulolytic activity and helps in channeling of intermediates. Appl Environ Microbiol 78:7447–7454
2. Bailey MJ (1988) A note on the use of dinitrosalicylic acid for determining the products of enzymatic reactions. Appl Microbiol Biotechnol 29:494–496
3. Bergquist PL, Gibbs MD, Morris DD, Junior Te’o VS, Saul DJ, Morgan HW (1999) Molecular diversity of thermophilic cellulolytic and hemicellulolytic bacteria. FEMS Microbiol Ecol 28:99–110
4. Bornscheuer UT, Huisman GW, Kazlauskas RJ, Lutz S, Moore JC, Robins K (2012) Engineering the third wave of biocatalysis. Nature 485:185–194
5. Bornscheuer U, Buchholz K, Seibel J (2014) Enzymatic degradation of (ligno)cellulose. Angew Chem Int Ed Engl 53:10876–10893
6. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
7. Bülow L, Ljungcrantz P, Mosbach K (1985) Preparation of a soluble bifunctional enzyme by gene fusion. Biotechnology 3:821–823
8. Chow J, Kovacic F, Dall Antonia Y, Krauss U, Fersini F, Schmeisser C et al (2012) The metagenome-derived enzymes LipS and LipT increase the diversity of known lipases. PLoS One 7:e47665
9. Conrado RJ, Varner JD, DeLisa MP (2008) Engineering the spatial organization of metabolic enzymes: mimicking nature’s synergy. Curr Opin Biotechnol 19:492–499
10. da Costa Sousa L, Chundawat SP, Balan V, Dale BE (2009) ‘Cradle-to-grave’ assessment of existing lignocellulose pretreatment technologies. Curr Opin Biotechnol 20:339–347
11. Elleuche S (2015) Bringing functions together with fusion enzymes-from nature’s inventions to biotechnological applications. Appl Microbiol Biotechnol 99:1545–1556
12. Elleuche S, Schröder C, Sahm K, Antranikian G (2014) Extremozymes-biocatalysts with unique properties from extremophilic microorganisms. Curr Opin Biotechnol 29C:116–123
13. Fan Z, Wagschal K, Chen W, Montross MD, Lee CC, Yuan L (2009a) Multimeric hemicellulases facilitate biomass conversion. Appl Environ Microbiol 75:1754–1757
14. Fan Z, Wagschal K, Lee CC, Kong Q, Shen KA, Maiti IB et al (2009b) The construction and characterization of two xylan-degrading chimeric enzymes. Biotechnol Bioeng 102:684–692
15. Graham JE, Clark ME, Nadler DC, Huffer S, Chokhawala HA, Rowland SE et al (2011) Identification and characterization of a multidomain hyperthermophilic cellulase from an archaeal enrichment. Nat Commun 2:375
16. Hong SY, Lee JS, Cho KM, Math RK, Kim YH, Hong SJ et al (2007) Construction of the bifunctional enzyme cellulase-β-glucosidase from the hyperthermophilic bacterium Thermotoga maritima. Biotechnol Lett 29:931–936
17. Horn U, Strittmatter W, Krebber A, Knüpfer U, Kujau M, Wenderoth R et al (1996) High volumetric yields of functional dimeric mini antibodies in Escherichia coli, using an optimized expression vector and high-cell-density fermentation under non-limited growth conditions. Appl Microbiol Biotechnol 46:524–532
18. Hu J, Arantes V, Pribowo A, Saddler JN (2013) The synergistic action of accessory enzymes enhances the hydrolytic potential of a “cellulase mixture” but is highly substrate specific. Biotechnol Biofuels 6:112
19. Kang YM, Kim MK, An JM, Haque MA, Cho KM (2015) Metagenomics of un-culturable bacteria in cow rumen: construction of cel9E–xyn10A fusion gene by site-directed mutagenesis. J Mol Catal B Enzym 113:29–38
20. Khandeparker R, Numan MT (2008) Bifunctional xylanases and their potential use in biotechnology. J Ind Microbiol Biotechnol 35:635–644
21. Klippel B (2011) Charakterisierung von celluloytischen und xylanolytischen Enzymen aus dem thermophilen anaeroben Bakterium Fervidobacterium gondwanense. PhD thesis. Hamburg University of Technology, Hamburg, Germany. MBV Berlin. ISBN: 9783866649569
22. Klippel B, Antranikian G (2011) Lignocellulose converting enzymes from thermophiles. In: Horikoshi K, Antranikian G, Bull A, Robb F, Stetter K (eds) Extremophiles handbook, vol 1. Springer, Tokyo, pp 443–474
23. Kuhad RC, Gupta R, Singh A (2011) Microbial cellulases and their industrial applications. Enzym Res 2011:280696-1–280696-10. doi:10.4061/2011/280696
24. Lee HL, Chang CK, Teng KH, Liang PH (2011) Construction and characterization of different fusion proteins between cellulases and β-glucosidase to improve glucose production and thermostability. Bioresour Technol 102:3973–3976
25. Marquardt T, von der Heyde A, Elleuche S (2014) Design and establishment of a vector system that enables production of multifusion proteins and easy purification by a two-step affinity chromatography approach. J Microbiol Methods 105C:47–50
26. Morais S, Morag E, Barak Y, Goldman D, Hadar Y, Lamed R et al (2012) Deconstruction of lignocellulose into soluble sugars by native and designer cellulosomes. MBio 3(6):e00508–e00512
27. Resch MG, Donohoe BS, Baker JO, Decker SR, Bayer EA, Beckham GT et al (2013) Fungal cellulases and complexed cellulosomal enzymes exhibit synergistic mechanisms in cellulose deconstruction. Energy Environ Sci 6:1858–1867
28. Ribeiro LF, Furtado GP, Lourenzoni MR, Costa-Filho AJ, Santos CR, Nogueira SC et al (2011) Engineering bifunctional laccase-xylanase chimeras for improved catalytic performance. J Biol Chem 286:43026–43038
29. Riedel K, Bronnenmeier K (1998) Intramolecular synergism in an engineered exo-endo-1,4-β-glucanase fusion protein. Mol Microbiol 28:675–767
30. Rizk M, Antranikian G, Elleuche S (2012) End-to-end gene fusions and their impact on the production of multifunctional biomass degrading enzymes. Biochem Biophys Res Commun 428:1–5
31. Rizk M, Elleuche S, Antranikian G (2015) Generating bifunctional fusion enzymes composed of heat-active endoglucanase (Cel5A) and endoxylanase (XylT). Biotechnol Lett 37:139–145
32. Schmidt TG, Skerra A (2007) The Strep-tag system for one-step purification and high-affinity detection or capturing of proteins. Nat Protoc 2:1528–1535
33. Schröder C, Elleuche S, Blank S, Antranikian G (2014) Characterization of a heat-active archaeal β-glucosidase from a hydrothermal spring metagenome. Enzym Microb Technol 57:48–54
34. Zhao L, Pang Q, Xie J, Pei J, Wang F, Fan S (2013) Enzymatic properties of Thermoanaerobacterium thermosaccharolyticum β-glucosidase fused to Clostridium cellulovorans cellulose binding domain and its application in hydrolysis of microcrystalline cellulose. BMC Biotechnol 13:101