Deconstruction of lignocellulose into soluble sugars by native and designer cellulosomes

mBio

Volumn 3, Issue 6, 2012, Pages

  Moraïs, Sarah ^a,b   Morag, Ely ^c   Barak, Yoav ^a   Goldman, Dan ^d   Hadar, Yitzhak ^b   Lamed, Raphael ^e   Shoham, Yuval ^d   Wilson, David B ^f   Bayera, Edward A ^a  

^a WEIZMANN INSTITUTE OF SCIENCE   (Israel)

^b HEBREW UNIVERSITY OF JERUSALEM   (Israel)

^c Designer Energy   (Israel)

^d TECHNION ISRAEL INSTITUTE OF TECHNOLOGY   (Israel)

^e TEL AVIV UNIVERSITY   (Israel)

^f Department of Obstetrics Gynecology   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BETA GLUCOSIDASE; CELLULASE; CELLULOSE; CELLULOSOME; COHESIN; DESIGNER CELLULOSOME; HEMICELLULOSE; LIGNIN; LIGNOCELLULOSE; UNCLASSIFIED DRUG; XYLAN ENDO 1,3 BETA XYLOSIDASE;

ARTICLE; BINDING AFFINITY; BIODEGRADATION; CARBOXY TERMINAL SEQUENCE; CHEMICAL PARAMETERS; CLOSTRIDIUM THERMOCELLUM; COMPLEX FORMATION; CONTROLLED STUDY; ENZYME DEGRADATION; ENZYME SPECIFICITY; ENZYME SUBSTRATE COMPLEX; HYDROLYSIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; RECALCITRANCE; STRAW; THERMOBIFIDA FUSCA; WHEAT; WILD TYPE;

BACTERIA (MICROORGANISMS); CLOSTRIDIUM THERMOCELLUM; THERMOBIFIDA FUSCA; TRITICUM AESTIVUM;

EID: 84872142750     PISSN: 21612129     EISSN: 21507511     Source Type: Journal    
DOI: 10.1128/mBio.00508-12     Document Type: Article

References (47)

1. Kim S, Dale B. 2004. Global potential bioethanol production from wasted crops and crop residues. Biomass Bioenerg. 26:361-375.
2. Wilson DB. 2012. Processive and nonprocessive cellulases for biofuel production-lessons from bacterial genomes and structural analysis. Appl. Microbiol. Biotechnol. 93:497-502.
3. Fierobe HP, et al. 2001. Design and production of active cellulosome chimeras: selective incorporation of dockerin-containing enzymes into defined functional complexes. J. Biol. Chem. 276:21257-21261.
4. Fierobe H-P, et al. 2002. Designer nanosomes: selective engineering of dockerin-containing enzymes into chimeric scaffoldins to form defined nanoreactors, p 113-123. In Teeri TT, Svensson B, Gilbert HJ, Feizi T (ed), Carbohydrate bioengineering: interdisciplinary approaches. Royal Society of Chemistry, Cambridge, United Kingdom.
5. Fierobe HP, et al. 2005. Action of designer cellulosomes on homogeneous versus complex substrates: controlled incorporation of three distinct enzymes into a defined tri-functional scaffoldin. J. Biol. Chem. 280: 16325-16334.
6. Kataeva I, Guglielmi G, Béguin P. 1997. Interaction between Clostridium thermocellum endoglucanase CelD and polypeptides derived from the cellulosome-integrating protein CipA: stoichiometry and cellulolytic activity of the complexes. Biochem. J. 326:617-624.
7. Mingardon F, Chanal A, Tardif C, Bayer EA, Fierobe HP. 2007. Exploration of new geometries in cellulosome-like chimeras. Appl. Environ. Microbiol. 73:7138-7149.
8. Murashima K, et al. 2002. Heterologous production of Clostridium cellulovorans engB, using protease-deficient Bacillus subtilis, and preparation of active recombinant cellulosomes. J. Bacteriol. 184:76-81.
9. Caspi J, et al. 2010. Thermobifida fusca exoglucanase Cel6B is incompatible with the cellulosomal mode in contrast to endoglucanase Cel6A. Syst. Synth. Biol. 4:193-201.
10. Caspi J, et al. 2009. Effect of linker length and dockerin position on conversion of a Thermobifida fusca endoglucanase to the cellulosomal mode. Appl. Environ. Microbiol. 75:7335-7342.
11. Caspi J, et al. 2008. Conversion of noncellulosomal Thermobifida fusca free exoglucanases into cellulosomal components: comparative impact on cellulose-degrading activity. J. Biotechnol. 135:351-357.
12. Caspi J, et al. 2006. Thermobifida fusca family 6 cellulases as potential designer cellulosome components. Biocatal. Biotransform. 24:3-12.
13. Moraïs S, et al. 2010. Cellulase-xylanase synergy in designer cellulosomes for enhanced degradation of a complex cellulosic substrate. mBio 1:e00285-e00310.
14. Moraïs S, et al. 2010. Contribution of a xylan-binding module to the degradation of a complex cellulosic substrate by designer cellulosomes. Appl. Environ. Microbiol. 76:3787-3796.
15. Morag E, Halevy I, Bayer EA, lamed R. 1991. Isolation and properties of a major cellobiohydrolase from the cellulosome of Clostridium thermocellum. J. Bacteriol. 173:4155-4162.
16. Irwin D, Jung ED, Wilson DB. 1994. Characterization and sequence of a Thermomonospora fusca xylanase. Appl. Environ. Microbiol. 60:763-770.
17. Kim JH, Irwin D, Wilson DB. 2004. Purification and characterization of Thermobifida fusca xylanase 10B. Can. J. Microbiol. 50:835-843.
18. Hu J, Arantes V, Saddler JN. 2011. The enhancement of enzymatic hydrolysis of lignocellulosic substrates by the addition of accessory enzymes such as xylanase: is it an additive or synergistic effect? Biotechnol. Biofuels 4:36.
19. Spiridonov NA, Wilson DB. 2001. Cloning and biochemical characterization of BglC, a beta-glucosidase from the cellulolytic actinomycete Thermobifida fusca. Curr. Microbiol. 42:295-301.
20. Moraïs S, et al. 2011. Assembly of xylanases into designer cellulosomes promotes efficient hydrolysis of the xylan component of a natural recalcitrant cellulosic substrate. mBio 2(6):e00233-e00311.
21. Wang WK, Kruus K, Wu JH. 1993. Cloning and DNA sequence of the gene coding for Clostridium thermocellum cellulase SS (CelS), a major cellulosome component. J. Bacteriol. 175:1293-1302.
22. Ding SY, et al. 2001. Cellulosomal scaffoldin-like proteins from Ruminococcus flavefaciens. J. Bacteriol. 183:1945-1953.
23. Barak Y, et al. 2005. Matching fusion-protein systems for affinity analysis of two interacting families of proteins: the cohesin-dockerin interaction. J. Mol. Recognit. 18:491-501.
24. Keller FA, Hamilton JE, Nguyen QA. 2003. Microbial pretreatment of biomass: potential for reducing severity of thermochemical biomass pretreatment. Appl. Biochem. Biotechnol. 105-108:27-41.
25. Shi J, Sharma-Shivappa R, Chinn M, Howell N. 2009. Effect of microbial pretreatment on enzymatic hydrolysis and fermentation of cotton stalks for ethanol production. Biomass Bioenerg. 33:88-96.
26. Heiss-Blanquet S, Zheng D, Lopes Ferreira N, Lapierre C, Baumberger S. 2011. Effect of pretreatment and enzymatic hydrolysis of wheat straw on cell wall composition, hydrophobicity and cellulase adsorption. Bioresour. Technol. 102:5938-5946.
27. Qing Q, Yang B, Wyman CE. 2010. Xylooligomers are strong inhibitors of cellulose hydrolysis by enzymes. Bioresour. Technol. 101:9624-9630.
28. Qing Q, Wyman CE. 2011. Supplementation with xylanase and β-xylosidase to reduce xylo-oligomer and xylan inhibition of enzymatic hydrolysis of cellulose and pretreated corn stover. Biotechnol. Biofuels 4:18.
29. Zhang J, Tuomainen P, Siika-Aho M, Viikari L. 2011. Comparison of the synergistic action of two thermostable xylanases from GH families 10 and 11 with thermostable cellulases in lignocellulose hydrolysis. Bioresour. Technol. 102:9090-9095.
30. Talebnia F, Karakashev D, Angelidaki I. 2010. Production of bioethanol from wheat straw: an overview on pretreatment, hydrolysis and fermentation. Bioresour. Technol. 101:4744-4753.
31. Pallapolu VR, et al. 2011. Effects of enzyme loading and β-glucosidase supplementation on enzymatic hydrolysis of switchgrass processed by leading pretreatment technologies. Bioresour. Technol. 102: 11115-11120.
32. Gefen G, Anbar M, Morag E, lamed R, Bayer EA. 2012. Enhanced cellulose degradation by targeted integration of a cohesin-fused betaglucosidase into the Clostridium thermocellum cellulosome. Proc. Natl. Acad. Sci. U.S.A. 109:10298-10303.
33. Maki ML, Armstrong L, Leung KT, Qin W. 24 August 2012. Increased expression of β-glucosidase A in Clostridium thermocellum 27405 significantly increases cellulase activity. Bioengineered. [Epub ahead of print.] http://dx.doi.org/10.4161/bioe.21951.
34. Platt M, Hadar Y, Henis Y, Chet I. 1983. Increased degradation of straw by Pleurotus ostreatus sp. "florida". Eur. J. Appl. Microbiol. Biotechnol. 17:140-142.
35. Gao D, et al. 2011. Hemicellulases and auxiliary enzymes for improved conversion of lignocellulosic biomass to monosaccharides. Biotechnol. Biofuels 4:5.
36. Irwin D, et al. 1998. Roles of the catalytic domain and two cellulose binding domains of Thermomonospora fusca E4 in cellulose hydrolysis. J. Bacteriol. 180:1709-1714.
37. Mingardon F, et al. 2007. Incorporation of fungal cellulases in bacterial minicellulosomes yields viable, synergistically acting cellulolytic complexes. Appl. Environ. Microbiol. 73:3822-3832.
38. Haimovitz R, et al. 2008. Cohesin-dockerin microarray: diverse specificities between two complementary families of interacting protein modules. Proteomics 8:968-979.
39. Irwin DC, Zhang S, Wilson DB. 2000. Cloning, expression and characterization of a family 48 exocellulase, Cel48A, from Thermobifida fusca. Eur. J. Biochem. 267:4988-4997.
40. Tabka MG, Herpoël-Gimbert I, Monod F, Asther M, Sigoillot JC. 2006. Enzymatic saccharification of wheat straw for bioethanol production by a combined cellulase xylanase and feruloyl esterase treatment. Enzyme Microb. Technol. 39:897-902.
41. Miller GL. 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Biochem. 31:426-428.
42. Ardon O, Kerem Z, Hadar Y. 1998. Enhancement of lignin degradation and laccase activity in Pleurotus ostreatus by cotton Salk. Can. J. Microbiol. 44:676-680.
43. Kerem Z, Hadar Y. 1995. Effect of manganese on preferential degradation of lignin by Pleurotus ostreatus during solid-state fermentation. Appl. Environ. Microbiol. 61:3057-3062.
44. Platt M, Hadar Y, Chet I. 1984. Fungal activities involved in lignocellulose degradation by Pleurotus. Appl. Microbiol. Biotechnol. 20:150-154.
45. TAPPI (Technical Association of the Pulp and Paper Industry). 1994. Test methods. TAPPI Press, Atlanta, GA.
46. Morais S, Salama-Alber O, Barak Y, Hadar Y, Wilson DB, Lamed R, Shoham Y, Bayer EA. 2012. Functional association of catalytic and ancillary modules dictates enzymatic activity in glycoside hydrolase family 43 beta-xylosidase. J. Biol. Chem. 287:9213-9221.
47. Shi J, Sharma-Shivappa R, Chinn M, Howell N. 2009. Effect of microbial pretreatment on enzymatic hydrolysis and fermentation of cotton stalks for ethanol production. Biomass Bioenergy 33:88-96.