Simple method for markerless gene deletion in multidrug-resistant Acinetobacter baumannii

Applied and Environmental Microbiology

Volumn 81, Issue 10, 2015, Pages 3357-3368

  Oh, Man Hwan ^a   Lee, Je Chul ^b   Kim, Jungmin ^b   Choi, Chul Hee ^c   Han, Kyudong ^a  

^a Dankook University   (South Korea)

^b Kyungpook National University   (South Korea)

^c Chungnam National University   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBIOTICS; CLONING; DNA; GENETIC ENGINEERING; POLYMERASE CHAIN REACTION; VECTORS;

ANTIBIOTIC RESISTANCE; DOWNSTREAM REGION; HOMOLOGOUS RECOMBINATION; IRON ACQUISITION; MULTIDRUG RESISTANTS; RECOMBINANT BACTERIA; RESTRICTION DIGESTION; SUICIDE VECTORS;

GENES;

ANTIBIOTIC RESISTANCE; BACTERIUM; DISEASE VECTOR; GENE EXPRESSION; MUTATION; PLASMID; RECOMBINATION;

ACINETOBACTER BAUMANNII;

ANTIINFECTIVE AGENT; BACTERIAL PROTEIN; GENETIC MARKER;

ACINETOBACTER BAUMANNII; ACINETOBACTER INFECTION; ANTIBIOTIC RESISTANCE; DRUG EFFECTS; EVALUATION STUDY; GENE DELETION; GENETIC MARKER; GENETIC PROCEDURES; GENETICS; HUMAN; METABOLISM; MICROBIOLOGY;

ACINETOBACTER BAUMANNII; ACINETOBACTER INFECTIONS; ANTI-BACTERIAL AGENTS; BACTERIAL PROTEINS; DRUG RESISTANCE, BACTERIAL; GENE DELETION; GENETIC MARKERS; GENETIC TECHNIQUES; HUMANS;

EID: 84930025716     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.03975-14     Document Type: Article

References (29)

1. Bergogne-Bérézin E, Towner KJ. 1996. Acinetobacter spp as nosocomial pathogens: microbiological, clinical, and epidemiological features. Clin Microbiol Rev 9:148-165.
2. Dijkshoorn L, Nemec A, Seifert H. 2007. An increasing threat in hospitals:multidrug-resistant Acinetobacter baumannii Nat Rev Microbiol 5:939-951. http://dx.doi.org/10.1038/nrmicro1789.
3. Seifert H, Strate A, Pulverer G. 1995. Nosocomial bacteremia due to Acinetobacter baumannii Clinical features, epidemiology, and predictors of mortality. Medicine 74:340-349.
4. Durante-Mangoni E, Zarrilli R. 2011. Global spread of drug-resistant Acinetobacter baumannii Future Microbiol 6:407-422. http://dx.doi.org /10.2217/fmb.11.23.
5. Smith MG, Gianoulis TA, Pukatzki S, Mekalanos JJ, Ornston LN, Gerstein M, Snyder M. 2007. New insights into Acinetobacter baumannii pathogenesis revealed by high-density pyrosequencing and transposon mutagenesis Genes Dev 21:601-614. http://dx.doi.org/10.1101/gad.1510307.
6. Vallenet D, Nordmann P, Barbe VR, Poirel L, Mangenot S, Bataille E, Dossat C, Gas S, Kreimeyer A, Lenoble P, Oztas S, Poulain J, Segurens BA, Robert C, Abergel C, Claverie JM, Raoult D, Médigue C, Weissenbach J, Cruveiller SP. 2008. Comparative analysis of acinetobacters: three genomes for three lifestyles PLoS One 3:e1805. http://dx.doi.org/10.1371/journal.pone.0001805.
7. Rumbo-Feal S, Gomez MJ, Gayoso C, Alvarez-Fraga L, Cabral MP, Aransay AM, Rodriguez-Ezpeleta N, Fullaondo A, Valle J, Tomas M, Bou G, Poza M. 2013. Whole transcriptome analysis of Acinetobacter baumannii assessed by RNA-sequencing reveals different mRNA expression profiles in biofilm compared to planktonic cells PLoS One 8:e72968. http://dx.doi.org/10.1371/journal.pone.0072968.
8. Roca I, Marti S, Espinal P, Martinez P, Gibert I, Vila J. 2009. CraA, a major facilitator superfamily efflux pump associated with chloramphenicol resistance in Acinetobacter baumannii Antimicrob Agents Chemother 53:4013-4014. http://dx.doi.org/10.1128/AAC.00584-09.
9. Penwell WF, Arivett BA, Actis LA. 2012. The Acinetobacter baumannii entA gene located outside the acinetobactin cluster is critical for siderophore production, iron acquisition, and virulence PLoS One 7:e36493. http://dx.doi.org/10.1371/journal.pone.0036493.
10. Camarena L, Bruno V, Euskirchen G, Poggio S, Snyder M. 2010. Molecular mechanisms of ethanol-induced pathogenesis revealed by RNA-sequencing PLoS Pathog 6:e1000834. http://dx.doi.org/10.1371/journal.ppat.1000834.
11. Aranda J, Poza M, Pardo BG, Rumbo S, Rumbo C, Parreira JR, Rodriguez-Velo P, Bou G. 2010. A rapid and simple method for constructing stable mutants of Acinetobacter baumannii BMC Microbiol 10:279. http://dx.doi.org/10.1186/1471-2180-10-279.
12. Bentancor LV, Camacho-Peiro A, Bozkurt-Guzel C, Pier GB, Maira-Litrán T. 2012. Identification of Ata, a multifunctional trimeric autotransporter of Acinetobacter baumannii J Bacteriol 194:3950-3960. http://dx.doi.org/10.1128/JB.06769-11.
13. Amin IM, Richmond GE, Sen P, Koh TH, Piddock LJV, Chua KL. 2013. A method for generating marker-less gene deletions in multidrugresistant Acinetobacter baumannii BMC Microbiol 13:158. http://dx.doi .org/10.1186/1471-2180-13-158.
14. Tucker AT, Nowicki EM, Boll JM, Knauf GA, Burdis NC, Trent MS, Davies BW. 2014. Defining gene-phenotype relationships in Acinetobacter baumannii through one-step chromosomal gene inactivation mBio 5(4):e01313-14. http://dx.doi.org/10.1128/mBio.01313-14.
15. Zimbler DL, Penwell WF, Gaddy JA, Menke SM, Tomaras AP, Connerly PL, Actis LA. 2009. Iron acquisition functions expressed by the human pathogen Acinetobacter baumannii Biometals 22:23-32. http://dx .doi.org/10.1007/s10534-008-9202-3.
16. Gaddy JA, Arivett BA, McConnell MJ, Lopez-Rojas R, Pachon J, Actis LA. 2012. Role of acinetobactin-mediated iron acquisition functions in the interaction of Acinetobacter baumannii strain ATCC 19606T with human lung epithelial cells, Galleria mellonella caterpillars, and mice Infect Immun 80:1015-1024. http://dx.doi.org/10.1128/IAI.06279-11.
17. Sambrook J, Russell DW. 2001. Molecular cloning: a laboratory manual, 3rd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
18. Lee HW, Koh YM, Kim J, Lee JC, Lee YC, Seol SY, Cho DT, Kim J. 2008. Capacity of multidrug-resistant clinical isolates of Acinetobacter baumannii to form biofilm and adhere to epithelial cell surfaces Clin Microbiol Infect 14:49-54. http://dx.doi.org/10.1111/j.1469-0691.2007.01842.x.
19. Philippe N, Alcaraz J-P, Coursange E, Geiselmann J, Schneider D. 2004. Improvement of pCVD442, a suicide plasmid for gene allele exchange in bacteria Plasmid 51:246-255. http://dx.doi.org/10.1016/j.plasmid.2004.02.003.
20. Eijkelkamp BA, Hassan KA, Paulsen IT, Brown MH. 2011. Investigation of the human pathogen Acinetobacter baumannii under iron limiting con- ditions BMC Genomics 12:126. http://dx.doi.org/10.1186/1471-2164-12-126.
21. Perez F, Hujer AM, Hujer KM, Decker BK, Rather PN, Bonomo RA. 2007. Global challenge of multidrug-resistant Acinetobacter baumannii Antimicrob Agents Chemother 51:3471-3484. http://dx.doi.org/10.1128/AAC.01464-06.
22. Gaddy JA, Tomaras AP, Actis LA. 2009. The Acinetobacter baumannii 19606 OmpA protein plays a role in biofilm formation on abiotic surfaces and in the interaction of this pathogen with eukaryotic cells Infect Immun 77:3150-3160. http://dx.doi.org/10.1128/IAI.00096-09.
23. Wandersman C, Delepelaire P. 2004. Bacterial iron sources: from siderophores to hemophores Annu Rev Microbiol 58:611-647. http://dx.doi .org/10.1146/annurev.micro.58.030603.123811.
24. Braun V. 2001. Iron uptake mechanisms and their regulation in pathogenic bacteria Int J Med Microbiol 291:67-79. http://dx.doi.org/10.1078/1438-4221-00103.
25. Lim YM, Shin KS, Kim J. 2007. Distinct antimicrobial resistance patterns and antimicrobial resistance-harboring genes according to genomic species of Acinetobacter isolates J Clin Microbiol 45:902-905. http://dx.doi .org/10.1128/JCM.01573-06.
26. Bouvet PJM, Grimont PAD. 1986. Taxonomy of the genus Acinetobacter with the recognition of Acinetobacter baumannii sp nov., Acinetobacter haemolyticus sp. nov., Acinetobacter johnsonii sp. nov., and Acinetobacter junii sp. nov. and emended descriptions of Acinetobacter calcoaceticus and Acinetobacter lwoffii. Int J Syst Evol Microbiol 36:228-240.
27. Piechaud M, Second L. 1951. Studies of 26 strains of Moraxella lwoffii Ann Inst Pasteur (Paris) 80:97-99.
28. Simon R, Priefer U, Pühler A. 1983. A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in gram negative bacteria Nat Biotechnol 1:784-791. http://dx.doi.org/10.1038/nbt1183-784.
29. Simon D, Chopin A. 1988. Construction of a vector plasmid family and its use for molecular cloning in Streptococcus lactis Biochimie 70:559-566. http://dx.doi.org/10.1016/0300-9084(88)90093-4.