Alternative to the soft-agar assay that permits high-throughput drug and genetic screens for cellular transformation

Proceedings of the National Academy of Sciences of the United States of America

Volumn 112, Issue 18, 2015, Pages 5708-5713

  Rotem, Asaf ^a   Janzer, Andreas ^a,d   Izar, Benjamin ^b,c   Ji, Zhe ^a   Doench, John G ^c   Garraway, Levi A ^b,c   Struhl, Kevin ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

^b DANA FARBER CANCER INSTITUTE   (United States)

^c BROAD INSTITUTE OF MIT AND HARVARD   (United States)

^d BAYER PHARMA AG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

AZELASTINE; INTERLEUKIN 6; NITAZOXANIDE; PHOSPHOTRANSFERASE INHIBITOR; ADENOSINE TRIPHOSPHATE; AGAR; ANTINEOPLASTIC AGENT;

ANCHORAGE INDEPENDENT GROWTH; ARTICLE; BIOASSAY; CANCER CELL; CANCER CHEMOTHERAPY; CANCER THERAPY; CELL DENSITY; CELL GROWTH; CELL PROLIFERATION; CELL TRANSFORMATION; CELL VIABILITY; COLONY FORMATION; CONTROLLED STUDY; CORRELATIONAL STUDY; DRUG SCREENING; FIBROBLAST; FOOD AND DRUG ADMINISTRATION; GENE IDENTIFICATION; GENETIC SCREENING; HIGH THROUGHPUT SCREENING; HUMAN; HUMAN CELL; IN VITRO STUDY; IN VIVO STUDY; NON-TRANSFORMED CELL; ONCOGENE; OVARY CANCER; PERSONALIZED MEDICINE; PRIORITY JOURNAL; QUANTITATIVE ASSAY; SOFT AGAR ASSAY; SURFACE PROPERTY; TRANSFORMED CELL; BREAST NEOPLASMS; CELL SURVIVAL; CHEMISTRY; DRUG DESIGN; FEMALE; FLOW CYTOMETRY; MEDICINAL CHEMISTRY; OPEN READING FRAME; OVARIAN NEOPLASMS; PATHOLOGY; TUMOR CELL LINE;

ADENOSINE TRIPHOSPHATE; AGAR; ANTINEOPLASTIC AGENTS; BREAST NEOPLASMS; CELL LINE, TUMOR; CELL PROLIFERATION; CELL SURVIVAL; CELL TRANSFORMATION, NEOPLASTIC; CHEMISTRY, PHARMACEUTICAL; DRUG DESIGN; DRUG SCREENING ASSAYS, ANTITUMOR; FEMALE; FIBROBLASTS; FLOW CYTOMETRY; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; OPEN READING FRAMES; OVARIAN NEOPLASMS;

EID: 84929492452     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1505979112     Document Type: Article

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