Evaluation of the NucliSens EasyQ v2.0 assay in comparison with the Roche Amplicor v1.5 and the Roche CAP/CTM HIV-1 test v2.0 in quantification of C-Clade HIV-1 in plasma

PLoS ONE

Volumn 9, Issue 8, 2014, Pages

  Muenchhoff, Maximilian ^a,b   Madurai, Savathee ^c   Hempenstall, Allison Jo ^a   Adland, Emily ^a   Carlqvist, Anna ^a   Moonsamy, Angeline ^b   Jaggernath, Manjeetha ^b   Mlotshwa, Busisiwe ^b   Siboto, Emma ^b   Ndung'U, Thumbi ^b,d,e   Goulder, Philip Jeremy Renshaw ^a,b,e  

^a UNIVERSITY OF OXFORD   (United Kingdom)

^b UNIVERSITY OF KWAZULU NATAL   (South Africa)

^c Global Clinical and Viral Laboratories ^*  (South Africa)

^d MAX PLANCK INSTITUTE FOR INFECTION BIOLOGY   (Germany)

^e HARVARD UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADULT; ANTIVIRAL RESISTANCE; ARTICLE; CD4 LYMPHOCYTE COUNT; CLADISTICS; COHORT ANALYSIS; CONTROLLED STUDY; DIAGNOSTIC KIT; DIAGNOSTIC TEST ACCURACY STUDY; FEMALE; HIGHLY ACTIVE ANTIRETROVIRAL THERAPY; HIV TEST; HUMAN; HUMAN CELL; HUMAN IMMUNODEFICIENCY VIRUS 1; HUMAN IMMUNODEFICIENCY VIRUS 1 INFECTION; INTERMETHOD COMPARISON; MAJOR CLINICAL STUDY; NONHUMAN; QUANTITATIVE ANALYSIS; SOUTH AFRICAN; VIREMIA; VIRUS DETECTION; VIRUS LOAD; BLOOD; COMPARATIVE STUDY; EVALUATION STUDY; GENETICS; GENOTYPE; HIV INFECTIONS; ISOLATION AND PURIFICATION; PHYSIOLOGY; PROCEDURES; SENSITIVITY AND SPECIFICITY;

ANTIRETROVIRUS AGENT;

ADULT; ANTI-RETROVIRAL AGENTS; COHORT STUDIES; FEMALE; GENOTYPE; HIV INFECTIONS; HIV-1; HUMANS; SENSITIVITY AND SPECIFICITY; VIRAL LOAD;

EID: 84929278683     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0103983     Document Type: Article

References (23)

1. Mellors JW, Rinaldo CR Jr, Gupta P, White RM, Todd JA, et al. (1996) Prognosis in HIV-1 infection predicted by the quantity of virus in plasma. Science 272: 1167-1170. (Pubitemid 26001148)
2. Swanson P, de Mendoza C, Joshi Y, Golden A, Hodinka RL, et al. (2005) Impact of human immunodeficiency virus type 1 (HIV-1) genetic diversity on performance of four commercial viral load assays: LCx HIV RNA Quantitative, AMPLICOR HIV-1 MONITOR v1.5, VERSANT HIV-1 RNA 3.0, and NucliSens HIV-1 QT. J Clin Microbiol 43: 3860-3868. (Pubitemid 41129673)
3. Xu S, Song A, Nie J, Li X, Meng S, et al. (2012) Comparison between the automated Roche Cobas AmpliPrep/Cobas TaqMan HIV-1 test version 2.0 assay and its version 1 and Nuclisens HIV-1 EasyQ version 2.0 assays when measuring diverse HIV-1 genotypes in China. J Clin Virol 53: 33-37.
4. Stevens W, Wiggill T, Horsfield P, Coetzee L, Scott LE (2005) Evaluation of the NucliSens EasyQ assay in HIV-1-infected individuals in South Africa. J Virol Methods 124: 105-110. (Pubitemid 40138785)
5. Bourlet T, Signori-Schmuck A, Roche L, Icard V, Saoudin H, et al. (2011) HIV-1 load comparison using four commercial real-time assays. J Clin Microbiol 49: 292-297.
6. Hemelaar J, Gouws E, Ghys PD, Osmanov S (2011) Global trends in molecular epidemiology of HIV-1 during 2000-2007. AIDS 25: 679-689.
7. Rousseau CM, Birditt BA, McKay AR, Stoddard JN, Lee TC, et al. (2006) Large-scale amplification, cloning and sequencing of near full-length HIV-1 subtype C genomes. J Virol Methods 136: 118-125. (Pubitemid 44081956)
8. Bland JM, Altman DG (1986) Statistical methods for assessing agreement between two methods of clinical measurement. Lancet 1: 307-310. (Pubitemid 16134762)
9. WHO (2013) The use of antiretroviral drugs for treating and preventing HIV infection: recommendations for a public health approach. Geneva. Available: http://www.who.int/hiv/pub/guidelines/arv2013/. Accessed 2013 Sept 30.
10. Church D, Gregson D, Lloyd T, Klein M, Beckthold B, et al. (2011) Comparison of the RealTime HIV-1, COBAS TaqMan 48 v1.0, Easy Q v1.2, and Versant v3.0 assays for determination of HIV-1 viral loads in a cohort of Canadian patients with diverse HIV subtype infections. J Clin Microbiol 49: 118-124.
11. Gomes P, Carvalho AP, Diogo I, Goncalves F, Costa I, et al. (2013) Comparison of the NucliSENS EasyQ HIV-1 v2.0 with Abbott m2000rt RealTime HIV-1 assay for plasma RNA quantitation in different HIV-1 subtypes. J Virol Methods 193: 18-22.
12. Gottesman BS, Grosman Z, Lorber M, Levi I, Shitrit P, et al. (2004) Measurement of HIV RNA in patients infected by subtype C by assays optimized for subtype B results in an underestimation of the viral load. J Med Virol 73: 167-171. (Pubitemid 38542668)
13. Gottesman BS, Grossman Z, Lorber M, Levi I, Shitrit P, et al. (2006) Comparative performance of the Amplicor HIV-1 Monitor Assay versus NucliSens EasyQ in HIV subtype C-infected patients. J Med Virol 78: 883-887. (Pubitemid 43856694)
14. Holguin A, Lopez M, Molinero M, Soriano V (2008) Performance of three commercial viral load assays, Versant human immunodeficiency virus type 1 (HIV-1) RNA bDNA v3.0, Cobas AmpliPrep/Cobas TaqMan HIV-1, and NucliSens HIV-1 EasyQ v1.2, testing HIV-1 non-B subtypes and recombinant variants. J Clin Microbiol 46: 2918-2923.
15. Xu S, Song A, Nie J, Li X, Wang Y (2010) Performance of NucliSens HIV-1 EasyQ Version 2.0 compared with six commercially available quantitative nucleic acid assays for detection of HIV-1 in China. Mol Diagn Ther 14: 305-316.
16. Mouinga-Ondeme A, Mabika-Mabika A, Alalade P, Mongo AD, Sica J, et al. (2014) Significant impact of non-B HIV-1 variants genetic diversity in Gabon on plasma HIV-1 RNA quantitation. J Med Virol 86: 52-57.
17. Sizmann D, Glaubitz J, Simon CO, Goedel S, Buergisser P, et al. (2010) Improved HIV-1 RNA quantitation by COBAS AmpliPrep/COBAS TaqMan HIV-1 Test, v2.0 using a novel dual-target approach. J Clin Virol 49: 41-46.
18. Pas S, Rossen JW, Schoener D, Thamke D, Pettersson A, et al. (2010) Performance evaluation of the new Roche Cobas AmpliPrep/Cobas TaqMan HIV-1 test version 2.0 for quantification of human immunodeficiency virus type 1 RNA. J Clin Microbiol 48: 1195-1200.
19. Jennings C, Harty B, Granger S, Wager C, Crump JA, et al. (2012) Cross-platform analysis of HIV-1 RNA data generated by a multicenter assay validation study with wide geographic representation. J Clin Microbiol 50: 2737-2747.
20. Saune K, Delaugerre C, Raymond S, Nicot F, Boineau J, et al. (2013) Analytical sensitivity of three real-time PCR assays for measuring subtype B HIV-1 RNA. J Clin Virol 57: 80-83.
21. Do T, Duncan J, Butcher A, Liegler T (2011) Comparative frequencies of HIV low-level viremia between real-time viral load assays at clinically relevant thresholds. J Clin Virol 52 Suppl 1: S83-89.
22. Garrett NJ, Apea V, Nori A, Ushiro-Lumb I, Oliver AR, et al. (2012) Comparison of the rate and size of HIV-1 viral load blips with Roche COBAS TaqMan HIV-1 versions 1.0 and 2.0 and implications for patient management. J Clin Virol 53: 354-355.
23. Taylor N, Grabmeier-Pfistershammer K, Egle A, Greil R, Rieger A, et al. (2013) Cobas ampliprep/cobas TaqMan HIV-1 v2.0 assay: consequences at the cohort level. PLoS One 8: e74024.