Comparison of the NucliSENS EasyQ HIV-1 v2.0 with Abbott m2000rt RealTime HIV-1 assay for plasma RNA quantitation in different HIV-1 subtypes

Journal of Virological Methods

Volumn 193, Issue 1, 2013, Pages 18-22

  Gomes, Perpétua ^a,b,c   Carvalho, Ana Patrícia ^a   Diogo, Isabel ^a   Gonçalves, Fátima ^a   Costa, Inês ^a   Cabanas, Joaquim ^a   Camacho, Ricardo Jorge ^a,c  

^a HOSPITAL EGAS MONIZ   (Portugal)

^b INSTITUTO SUPERIOR DE CIÊNCIAS DA SAÚDE EGAS MONIZ   (Portugal)

^c UNIVERSIDADE NOVA DE LISBOA   (Portugal)

Author keywords

EasyQ HIV 1; HIV 1; M2000; Subtypes

Indexed keywords

VIRUS RNA;

ADOLESCENT; ADULT; AGED; ANALYTICAL EQUIPMENT; ARTICLE; AUTOMATION; CHILD; CONTROLLED STUDY; FEMALE; FLUORESCENCE ANALYSIS; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS 1; HUMAN IMMUNODEFICIENCY VIRUS 1 INFECTION; INFANT; INTERMETHOD COMPARISON; MAJOR CLINICAL STUDY; MALE; MOLECULAR BEACON; NONHUMAN; PRESCHOOL CHILD; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SCHOOL CHILD; VIRUS LOAD; VIRUS RECOMBINANT;

EASYQ HIV-1; HIV-1; M2000; SUBTYPES;

ADOLESCENT; ADULT; AGED; CHILD; CHILD, PRESCHOOL; FEMALE; HIV INFECTIONS; HIV-1; HUMANS; INFANT; MALE; MIDDLE AGED; MOLECULAR DIAGNOSTIC TECHNIQUES; PLASMA; RNA, VIRAL; VIRAL LOAD; YOUNG ADULT;

HUMAN IMMUNODEFICIENCY VIRUS 1;

EID: 84878829535     PISSN: 01660934     EISSN: 18790984     Source Type: Journal    
DOI: 10.1016/j.jviromet.2013.05.001     Document Type: Article

References (30)

1. Aldrich C., Hemelaar J. Global HIV-1 diversity surveillance. Trends Mol. Med. 2012, 18:691-694.
2. Amendola A., Bordi L., Angeletti C., Visco-Comandini U., Abbate I., Cappiello G., Budabbus M.A., Eljhawi O.A., Mehabresh M.I., Girardi E., Antinori A., Ippolito G., Capobianchi M.R. Underevaluation of HIV-1 plasma viral load by a commercially available assay in a cluster of patients infected with HIV-1 A/G circulating recombinant form (CRF02). J. Acquir. Immune Defic. Syndr. 2002, 31:488-494.
3. Amendola A., Coen S., Belladonna S., Pulvirenti F.R., Clemens J.M., Capobianchi M.R. Improving clinical laboratory efficiency: a time-motion evaluation of the Abbott m2000 RealTime and Roche COBAS AmpliPrep/COBAS TaqMan PCR systems for the simultaneous quantitation of HIV-1 RNA and HCV RNA. Clin. Chem. Lab. Med. 2011, 49:1283-1288.
4. Antunes R., Figueiredo S., Bartolo I., Pinheiro M., Rosado L., Soares I., Lourenco H., Taveira N. Evaluation of the clinical sensitivities of three viral load assays with plasma samples from a pediatric population predominantly infected with human immunodeficiency virus type 1 subtype G and BG recombinant forms. J. Clin. Microbiol. 2003, 41:3361-3367.
5. Armitage P., Berry G., Matthews J.N.S. Statistical methods in medical research 2002, Willey-Blackwell, Boston. 4th edition.
6. Bartolo I., Abecasis A.B., Borrego P., Barroso H., McCutchan F., Gomes P., Camacho R., Taveira N. Origin and epidemiological history of HIV-1 CRF14_BG. PLoS One 2011, 6:e24130.
7. Bland J.M., Altman D.G. Statistical methods for assessing agreement between two methods of clinical measurement. Lancet 1986, 1:307-310.
8. Bourlet T., Signori-Schmuck A., Roche L., Icard V., Saoudin H., Trabaud M.A., Tardy J.C., Morand P., Pozzetto B., Ecochard R., Andre P. HIV-1 load comparison using four commercial real-time assays. J. Clin. Microbiol. 2011, 49:292-297.
9. Carvalho A.P., Gomes P., Diogo I., Goncalves M.F., Cabanas J., Abecasis A., Lobo M.C., Camacho R. Molecular epidemiology of HIV-1 infection in Southern Portugal: results of a 92 weeks survey. 9th European AIDS Conference (EACS); 1st EACS Resistance & Pharmacology Workshop 2003.
10. Church D., Gregson D., Lloyd T., Klein M., Beckthold B., Laupland K., Gill M.J. Comparison of the RealTime HIV-1, COBAS TaqMan 48 v1.0, Easy Q v1. 2, and Versant v3. 0 assays for determination of HIV-1 viral loads in a cohort of Canadian patients with diverse HIV subtype infections. J. Clin. Microbiol. 2011, 49:118-124.
11. de Baar M.P., van Dooren M.W., de Rooij E., Bakker M., van Gemen B., Goudsmit J., de Ronde A. Single rapid real-time monitored isothermal RNA amplification assay for quantification of human immunodeficiency virus type 1 isolates from groups M, N, and O. J. Clin. Microbiol. 2001, 39:1378-1384.
12. Esteves A., Parreira R., Venenno T., Franco M., Piedade J., Germano dS, Canas-Ferreira W.F. Molecular epidemiology of HIV type 1 infection in Portugal: high prevalence of non-B subtypes. AIDS Res. Hum. Retroviruses 2002, 18:313-325.
13. Fernandes D., Carvalho A.P., Diogo I., Gomes P., Mansinho K., Camacho R.J. Prevalence of HIV-1 subtypes in Portugal: evolution between 2001 and 2010. 13th European AIDS Conference/EACS, 12-15 October 2011 2011.
14. Garcia-Diaz A., Clewley G.S., Booth C.L., Labett W., McAllister N., Geretti A.M. Comparative evaluation of the performance of the Abbott real-time human immunodeficiency virus type 1 (HIV-1) assay for measurement of HIV-1 plasma viral load following automated specimen preparation. J. Clin. Microbiol. 2006, 44:1788-1791.
15. Gottesman B.S., Grossman Z., Lorber M., Levi I., Shitrit P., Katzir M., Shahar E., Gottesman G., Chowers M. Comparative performance of the Amplicor HIV-1 Monitor Assay versus NucliSens EasyQ in HIV subtype C-infected patients. J. Med. Virol. 2006, 78:883-887.
16. Hemelaar J., Gouws E., Ghys P.D., Osmanov S. Global trends in molecular epidemiology of HIV-1 during 2000-2007. AIDS 2011, 25:679-689.
17. Hodara V., Monticelli A., Pampuro S., Salomon H., Jauregui R.H., Libonatti O. HIV-1 viral load: comparative evaluation of three commercially available assays in Argentina. Acta Physiol. Pharmacol. Ther. Latinoam. 1998, 48:107-113.
18. Holguin A., Lopez M., Molinero M., Soriano V. Performance of three commercial viral load assays, Versant human immunodeficiency virus type 1 (HIV-1) RNA bDNA v3.0, Cobas AmpliPrep/Cobas TaqMan HIV-1, and NucliSens HIV-1 EasyQ v1. 2, testing HIV-1 non-B subtypes and recombinant variants. J. Clin. Microbiol. 2008, 46:2918-2923.
19. Kim J.E., Beckthold B., Chen Z., Mihowich J., Malloch L., Gill M.J. Short communication: identification of a novel HIV type 1 subtype H/J recombinant in Canada with discordant HIV viral load (RNA) values in three different commercial assays. AIDS Res. Hum. Retroviruses 2007, 23:1309-1313.
20. Mellors J.W., Rinaldo C.R., Gupta P., White R.M., Todd J.A., Kingsley L.A. Prognosis in HIV-1 infection predicted by the quantity of virus in plasma. Science 1996, 272:1167-1170.
21. Peter J.B., Blum R.A. Monitoring HIV viral loads in the United States: recent trends and methodologies. J. Acquir. Immune Defic. Syndr. 2002, 30:261-262.
22. Plantier J.C., Leoz M., Dickerson J.E., De Oliveira F., Cordonnier F., Lemee V., Damond F., Robertson D.L., Simon F. A new human immunodeficiency virus derived from gorillas. Nat. Med. 2009, 15:871-872.
23. Robertson D.L., Anderson J.P., Bradac J.A., Carr J.K., Foley B., Funkhouser R.K., Gao F., Hahn B.H., Kalish M.L., Kuiken C., Learn G.H., Leitner T., McCutchan F., Osmanov S., Peeters M., Pieniazek D., Salminen M., Sharp P.M., Wolinsky S., Korber B. HIV-1 nomenclature proposal. Science 2000, 288:55-56.
24. Rouet F., Foulongne V., Viljoen J., Steegen K., Becquart P., Valea D., Danaviah S., Segondy M., Verhofstede C., Van de P.P. Comparison of the Generic HIV Viral Load assay with the Amplicor HIV-1 monitor v1.5 and Nuclisens HIV-1 EasyQ v1.2 techniques for plasma HIV-1 RNA quantitation of non-B subtypes: the Kesho Bora preparatory study. J. Virol. Methods 2010, 163:253-257.
25. Saag M.S., Holodniy M., Kuritzkes D.R., O'Brien W.A., Coombs R., Poscher M.E., Jacobsen D.M., Shaw G.M., Richman D.D., Volberding P.A. HIV viral load markers in clinical practice. Nat. Med. 1996, 2:625-629.
26. Segondy M., Izopet J., Pellegrin I., Montes B., Dumon B., Pasquier C., Peeters M., Fleury H.J., Puel J., Reynes J. Comparison of the Quantiplex HIV-1 RNA 2.0 assay with the Amplicor HIV-1 Monitor 1.0 assay for quantitation of levels of human immunodeficiency virus type 1 RNA in plasma of patients receiving stavudine-didanosine combination therapy. J. Clin. Microbiol. 1998, 36:3392-3395.
27. Thompson M.A., Aberg J.A., Cahn P., Montaner J.S., Rizzardini G., Telenti A., Gatell J.M., Gunthard H.F., Hammer S.M., Hirsch M.S., Jacobsen D.M., Reiss P., Richman D.D., Volberding P.A., Yeni P., Schooley R.T. Antiretroviral treatment of adult HIV infection: 2010 recommendations of the International AIDS Society-USA panel. JAMA 2010, 304:321-333.
28. van Zyl G.U., Korsman S.N., Maree L., Preiser W. NucliSens EasyQ HIV-1 V1.2 system: detection of human plasma-derived background signal. J. Virol. Methods 2010, 165:318-319.
29. Xu S., Song A., Li X., Nie J., Zhang C., Wang Y. Performance of the automated COBAS AmpliPrep/COBAS TaqMan HIV-1 test on a genetically diverse panel of specimens from China: comparison to the COBAS amplicor HIV-1 monitor test, v1.5. Intervirology 2010, 53:221-228.
30. Xu S., Song A., Nie J., Li X., Meng S., Zhang C., Wang Y. Comparison between the automated Roche Cobas AmpliPrep/Cobas TaqMan HIV-1 test version 2.0 assay and its version 1 and Nuclisens HIV-1 EasyQ version 2.0 assays when measuring diverse HIV-1 genotypes in China. J. Clin. Virol. 2012, 53:33-37.