Characterization of bovine interferon α1: Expression in yeast pichia pastoris, biological activities, and physicochemical characteristics

Journal of Interferon and Cytokine Research

Volumn 35, Issue 3, 2015, Pages 168-175

  Shao, Jianwei ^a   Cao, Chong ^a   Bao, Jun ^a,b   Liu, Hongtao ^a   Peng, Tongquan ^a   Gao, Mingchun ^a,b   Wang, Junwei ^a,b  

^a NORTHEAST AGRICULTURAL UNIVERSITY   (China)

^b Synergetic Innovation Center of Food Safety and Nutrition   (China)

Author keywords

[No Author keywords available]

Indexed keywords

ALPHA INTERFERON; ALPHA1 INTERFERON; AMINO ACID; ANTIVIRUS AGENT; GENOMIC DNA; POLYCLONAL ANTIBODY; RECOMBINANT BOVINE INTERFERON ALPHA1; RECOMBINANT PROTEIN; SIGNAL PEPTIDE; TRYPSIN; UNCLASSIFIED DRUG; BLOCKING ANTIBODY;

ANIMAL CELL; ANTIVIRAL ACTIVITY; ARTICLE; BIOLOGICAL ACTIVITY; BOVINE INTERFERON ALPHA GENE; CONTROLLED STUDY; GENE; GENE EXPRESSION; GENE SEQUENCE; GENE STRUCTURE; IN VITRO STUDY; KOMAGATAELLA PASTORIS; MDCK CELL LINE; NONHUMAN; PH; PHYLOGENY; PHYSICAL CHEMISTRY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; STRUCTURE ANALYSIS; VESICULOVIRUS; WESTERN BLOTTING; ANIMAL; BOVINE; CELL LINE; CHEMISTRY; DRUG EFFECTS; EPITHELIUM CELL; GENETICS; GENOME; HUMAN; LIVER; METABOLISM; PHYSIOLOGY; PICHIA; RABBIT;

BOVINAE; ORYCTOLAGUS CUNICULUS; PICHIA PASTORIS;

ANIMALS; ANTIBODIES, BLOCKING; ANTIVIRAL AGENTS; CATTLE; CELL LINE; EPITHELIAL CELLS; GENE EXPRESSION; GENOME; HUMANS; INTERFERON-ALPHA; LIVER; PHYLOGENY; PICHIA; RABBITS;

EID: 84929152534     PISSN: 10799907     EISSN: 15577465     Source Type: Journal    
DOI: 10.1089/jir.2013.0139     Document Type: Article

References (18)

1. Cao ZW. 2004. An effective method of Tricine-SDS-PAGE for separating the 1 kDa peptide. China Biotechnol 24:74-76.
2. Chaplin PJ, Entrican G, Gelder KI, Collins RA. 1996a. Cloning and biologic activities of a bovine interferon-a isolated from the epithelium of rotavirus-infected calf. J Interferon Cyto-kine Res 16:25-30.
3. Chaplin PJ, Parsons KR, Collins RA. 1996b. The cloning of cattle interferon-A subtypes isolated from the gut epithelium of rotavirus-infected calves. Immunogenetics 44:143-145.
4. Damasceno LM, Huang CJ, Batt CA. 2012. Protein secretion in Pichia pastoris and advances in protein production. Appl Microbiol Biotechnol 93:31-39.
5. Fish EN, Banejee NK, Stebbing N. 1989. The role of three domains in the biological activity of human IFN-a. J Interferon Res 9:97-114.
6. Fostern GR, Rodrigues O, Ghouze F, Schulte-Frohlinde E, Testa D, Liao MJ, Stark GR, Lead beater L, Thomas HC. 1996. Different relative activities of human cell-derived interferon subtypes: IFN-a8 has very high antiviral potency. J Interferon Cytokine Res 16:1027-1033.
7. Gresser I, Bandu MT, Brouty-boye D, Tovey M. 1974. Pronounced antiviral activity of human interferon on bovine and porcine cells. Nature 251:543-545.
8. Hai JU, Liang D, Guo G, Zhang J. 2003. Comparison of four methods to prepare Pichia genomic DNA for PCR. Tianjin Med J 31:270-272.
9. Hou F, Liu K, Chen P, et al. 2011. Antiviral activity of rChIFN-a against vesicular stomatitis virus and Newcastle disease virus: a novel recombinant chicken interferon-a showed high antiviral activity. Res Vet Sci 91:e73-e79.
10. Meager A. 2002. Biological assays for interferons. J Immunol Methods 261:21-36.
11. Oritani K, Kincade PW, Zhang C, Tomiyama Y, Matsuzawa Y. 2001. Type I interferons and limitin: a comparison of structures, receptors, and functions. Cytokine Growth Factor Rev 12:337-347.
12. Reed LJ, Muench H. 1938. A simple method of estimating fifty percent endpoints. Am J Hygiene 27:493-497.
13. Roberts RM, Liu L, Guo Q, Leaman D, Bixby J. 1998. The evolution of the type I interferons. J Interferon Cytokine Res 18:805-816.
14. Sambrook J, Fritsch EF, Maniatis T. Translated by Jin DY, Li MF, Hou YD. 1992. Molecular Cloning: A Laboratory Manual, 2nd ed. Beijing: Science Press, pp 16-68.
15. Tamura K, Petersonn D, Peterson N, Stecher G, Nei M, Kumar S. 2011. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731-2739.
16. Van Heuvel M, Bosveld IJ, Klaassen P, Zwarthoff EC, Trapman J. 1998. Structure-function analysis of murine interferonalpha: antiviral properties of novel hybrid interferons. J Interferon Res 8:5-14.
17. Wetzel R, Levine HL, Estell DA, Shire S, Finer-Moore J, Stroud RM, Bewley TA. 1982. Structure-function studies on human alpha interferon. In: Interferons. Academic Press: NY, pp 365-376.
18. Zhao X, Cheng G, Yan W, Liu M, He Y, Zheng Z. 2009. Characterization and virus-induced expression profiles of the porcine interferon-o multigene family. J Interferon Cytokine Res 10:687-693.