Lowering the quantification limit of the QubitTM RNA HS Assay using RNA spike-in

BMC Molecular Biology

Volumn 16, Issue 1, 2015, Pages

  Li, Xin ^a   Ben Dov, Iddo Z ^b   Mauro, Maurizio ^a   Williams, Zev ^a  

^a ALBERT EINSTEIN COLLEGE OF MEDICINE   (United States)

^b HADASSAH HEBREW UNIVERSITY MEDICAL CENTER   (Israel)

Author keywords

Lower quantification limit; Minimum RNA concentration; Plasma RNA; Qubit RNA HS Assay; RNA quantification; RNA spike in

Indexed keywords

QUANTUM DOT; RNA;

ARTICLE; BIOASSAY; BLOOD SAMPLING; FLUORESCENCE; HUMAN; LIMIT OF QUANTITATION; RNA ISOLATION; RNA QUANTIFICATION ASSAY; TROPHOBLAST; ULTRAVIOLET SPECTROPHOTOMETRY; VALIDATION STUDY; BLOOD; CHEMICAL ANALYSIS; PROCEDURES; REPRODUCIBILITY; SPECIES DIFFERENCE;

CHEMISTRY TECHNIQUES, ANALYTICAL; HUMANS; REPRODUCIBILITY OF RESULTS; RNA; SPECIES SPECIFICITY;

EID: 84929149745     PISSN: None     EISSN: 14712199     Source Type: Journal    
DOI: 10.1186/s12867-015-0039-3     Document Type: Article

References (24)

1. Koh W, Pan W, Gawad C, Fan HC, Kerchner GA, Wyss-Coray T, et al. Noninvasive in vivo monitoring of tissue-specific global gene expression in humans. Proc Natl Acad Sci U S A. 2014;111(20):7361-6.
2. Kosaka N, Izumi H, Sekine K, Ochiya T. microRNA as a new immune-regulatory agent in breast milk. Silence. 2010;1:7.
3. Menke TB, Warnecke JM. Improved conditions for isolation and quantification of RNA in urine specimens. Ann N Y Acad Sci. 2004;1022:185-9.
4. Michael A, Bajracharya SD, Yuen PS, Zhou H, Star RA, Illei GG, et al. Exosomes from human saliva as a source of microRNA biomarkers. Oral Dis. 2010;16(1):34-8.
5. Mitchell PS, Parkin RK, Kroh EM, Fritz BR, Wyman SK, Pogosova-Agadjanyan EL, et al. Circulating microRNAs as stable blood-based markers for cancer detection. Proc Natl Acad Sci U S A. 2008;105(30):10513-8.
6. Williams Z, Ben-Dov IZ, Elias R, Mihailovic A, Brown M, Rosenwaks Z, et al. Comprehensive profiling of circulating microRNA via small RNA sequencing of cDNA libraries reveals biomarker potential and limitations. Proc Natl Acad Sci U S A. 2013;110(11):4255-60.
7. Ben-Dov IZ, Tan YC, Morozov P, Wilson PD, Rennert H, Blumenfeld JD, et al. Urine microRNA as potential biomarkers of autosomal dominant polycystic kidney disease progression: description of miRNA profiles at baseline. PLoS One. 2014;9(1):e86856.
8. Deng Q, Ramskold D, Reinius B, Sandberg R. Single-cell RNA-seq reveals dynamic, random monoallelic gene expression in mammalian cells. Science. 2014;343(6167):193-6.
9. Hedegaard J, Thorsen K, Lund MK, Hein AM, Hamilton-Dutoit SJ, Vang S, et al. Next-generation sequencing of RNA and DNA isolated from paired fresh-frozen and formalin-fixed paraffin-embedded samples of human cancer and normal tissue. PLoS One. 2014;9(5):e98187.
10. Patel AP, Tirosh I, Trombetta JJ, Shalek AK, Gillespie SM, Wakimoto H, et al. Single-cell RNA-seq highlights intratumoral heterogeneity in primary glioblastoma. Science. 2014;344(6190):1396-401.
11. Ramskold D, Luo S, Wang YC, Li R, Deng Q, Faridani OR, et al. Full-length mRNA-Seq from single-cell levels of RNA and individual circulating tumor cells. Nat Biotechnol. 2012;30(8):777-82.
12. Shalek AK, Satija R, Adiconis X, Gertner RS, Gaublomme JT, Raychowdhury R, et al. Single-cell transcriptomics reveals bimodality in expression and splicing in immune cells. Nature. 2013;498(7453):236-40.
13. Trapnell C, Cacchiarelli D, Grimsby J, Pokharel P, Li S, Morse M, et al. The dynamics and regulators of cell fate decisions are revealed by pseudotemporal ordering of single cells. Nat Biotechnol. 2014;32(4):381-6.
14. Yick CY, Zwinderman AH, Kunst PW, Grunberg K, Mauad T, Chowdhury S, et al. Gene expression profiling of laser microdissected airway smooth muscle tissue in asthma and atopy. Allergy. 2014;69(9):1233-40.
15. Glasel JA. Validity of nucleic acid purities monitored by 260 nm/280nm absorbance ratios. Biotechniques. 1995;18(1):62-3.
16. Ingle JD, Crouch SR. Spectrochemical Analysis. Englewood Cliffs, N.J: Prentice Hall; 1988.
17. Desjardins PR, Conklin DS. Microvolume quantitation of nucleic acids. In: Ausubel FM, editor. Current protocols in molecular biology, vol. Appendix 3. 2011. p. 3J.
18. Jones LJ, Yue ST, Cheung CY, Singer VL. RNA quantitation by fluorescence-based solution assay: RiboGreen reagent characterization. Anal Biochem. 1998;265(2):368-74.
19. Le Pecq JB, Paoletti C. A new fluorometric method for RNA and DNA determination. Anal Biochem. 1966;17(1):100-7.
20. Labarca C, Paigen K. A simple, rapid, and sensitive DNA assay procedure. Anal Biochem. 1980;102(2):344-52.
21. Rye HS, Dabora JM, Quesada MA, Mathies RA, Glazer AN. Fluorometric assay using dimeric dyes for double- and single-stranded DNA and RNA with picogram sensitivity. Anal Biochem. 1993;208(1):144-50.
22. Dallwig J, Hagen D, Cheung C-y, Thomas G, Yue S: Methine-substituted cyanine dye compounds. In. US Patent US 7,776,529 B2; 2010.
23. Burgos KL, Javaherian A, Bomprezzi R, Ghaffari L, Rhodes S, Courtright A, et al. Identification of extracellular miRNA in human cerebrospinal fluid by next-generation sequencing. RNA. 2013;19(5):712-22.
24. Rykova EY, Wunsche W, Brizgunova OE, Skvortsova TE, Tamkovich SN, Senin IS, et al. Concentrations of circulating RNA from healthy donors and cancer patients estimated by different methods. Ann N Y Acad Sci. 2006;1075:328-33.