Lack of genetic structure and evidence for long-distance dispersal in ash (Fraxinus excelsior) populations under threat from an emergent fungal pathogen: implications for restorative planting

Tree Genetics and Genomes

Volumn 11, Issue 3, 2015, Pages

  Beatty, Gemma E ^a   Brown, James A ^a   Cassidy, Eamon M ^a   Finlay, Caroline M V ^a   McKendrick, Lorraine ^a   Montgomery, W Ian ^a   Reid, Neil ^a   Tosh, David G ^a   Provan, Jim ^a  

^a QUEEN'S UNIVERSITY BELFAST   (United Kingdom)

Author keywords

Gene flow; Genetic diversity; Inbreeding; Microsatellites; Replanting; Spatial genetic structure

Indexed keywords

CHALARA; FRAXINUS EXCELSIOR;

EID: 84929104323     PISSN: 16142942     EISSN: 16142950     Source Type: Journal    
DOI: 10.1007/s11295-015-0879-5     Document Type: Article

References (63)

1. Ashley MV (2010) Plant parentage, pollination and dispersal: how DNA microsatellites have altered the landscape. Crit Rev Plant Sci 29:148–161
2. Augspurger C (1983) Recruitment around tropical trees: changes in cohort distance with time. Oikos 40:189–196
3. Bacles CFE, Burczyk J, Lowe AJ, Ennos RA (2005) Historical and contemporary mating patterns in remnant populations of the forest tree Fraxinus excelsior L. Evolution 59:979–990
4. Bacles CFE, Lowe AJ, Ennos RA (2006) Effective seed dispersal across a fragmented landscape. Science 311:628
5. Bacles CFE, Ennos RA (2008) Paternity analysis of pollen-mediated gene flow for Fraxinus excelsior L. in a chronically fragmented landscape. Heredity 101:368–380
6. Brachet S, Jubier MF, Richard M, Jung-Muller B, Frascaria-Lacoste N (1999) Rapid identification of microsatellite loci using 5’ anchored PCR in common ash Fraxinus excelsior. Mol Ecol Notes 8:160–163
7. Connell JH (1971) On the role of natural enemies in preventing competitive exclusion in some marine animals and forest trees. In: den Boer PJ, Gradwell GR (eds) Dynamics of populations. Centre for Agricultural Publishing and Documentation, Wageningen, pp 298–312
8. Corander J, Waldmann P, Sillanpää MJ (2003) Bayesian analysis of genetic differentiation between populations. Genetics 163:367–374
9. DeSalle R, Amato G (2004) The expansion of conservation genetics. Nat Rev Genet 5:702–712
10. Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:1–15
11. Ebert D, Peakall R (2009a) Chloroplast simple sequence repeats (cpSSRs): technical resources and recommendations for expanding cpSSR discovery and applications to a wide array of plant species. Mol Ecol Resour 9:673–690
12. Ebert D, Peakall R (2009b) A new set of universal de novo sequencing primers for extensive coverage of noncoding chloroplast DNA: new opportunities for phylogenetic studies and cpSSR discovery. Mol Ecol Resour 9:777–783
13. Excoffier L, Smouse PE, Quattro JM (1992) Analysis of molecular variance inferred from metric distances among DNA haplotypes—application to human mitochondrial DNA restriction data. Genetics 131:479–491
14. Excoffier L, Lischer HEL (2010) Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows. Mol Ecol Resour 10:564–567
15. Ferrazzini D, Monteleoni I, Belletti P (2007) Genetic variability and divergence among Italian populations of common ash (Fraxinus excelsior L.). Ann For Sci 64:159–168
16. Forget PM, Wenny D (2005) How to elucidate seed fate? A review of methods used to study seed removal and secondary seed dispersal. In: Forget PM et al (eds) Seed fate: seed predation, seed dispersal and seedling establishment. CABI Publishing, Wallingford, pp 379–393
17. FRAXIGEN (2005) Ash species in Europe: biological characteristics and practical guidelines for sustainable use. Oxford Forestry Institute, Oxford
18. Garcia C, Jordano P, Godoy JA (2007) Contemporary pollen and seed dispersal in a Prunus mahaleb population: patterns in distance and direction. Mol Ecol 16:1947–1955
19. Godoy JA, Jordano P (2001) Seed dispersal by animals: exact identification of source trees with endocarp DNA microsatellites. Mol Ecol 10:2275–2283
20. Gérard PR, Temunovic M, Sannier J, Bertolino P, Dufour J, Frascaria-Lacoste N, Fernández-Manjarrés JF (2013) Chilled but not frosty: understanding the role of climate in the hybridization between the Mediterranean Fraxinus angustifolia Vahl and the temperate Fraxinus excelsior L. (Oleaceae) ash trees. J Biogeogr 40:835–846
21. Godefroid S, Piazza C, Rossi G et al (2011) How successful are plant species reintroductions? Biol Conserv 144:672–682
22. Goudet J (2001) Fstat, version 2.9.3, A program to estimate and test gene diversities and fixation indices. http://www2.unil.ch/popgen/softwares/fstat.htm
23. Grivet D, Robledo-Arnuncio JJ, Smouse PE, Sork VL (2009) Relative contribution of contemporary pollen and seed dispersal to the neighbourhood size of a seedling population of California valley oak (Quercus lobata, Née). Mol Ecol 16:3967–3979
24. Harbourne ME, Douglas GC, Waldren S, Hodkinson TR (2005) Characterization and primer development and amplification of chloroplast microsatellite regions of Fraxinus excelsior. J Plant Res 118:339–341
25. Hardy OJ, Vekemans X (2002) SPAGeDi: a versatile computer program to analyse spatial genetic structure at the individual or population levels. Mol Ecol Notes 2:618–620
26. Hardy OJ, Maggia L, Bandou E, Breyne P, Caron J, Chevallier MH, Doligez A, Dutech C, Kremer A, Latouche-Hallé C, Troispoux V, Veron V, Degen B (2006) Fine-scale genetic structure and gene dispersal inferences in 10 neotropical tree species. Mol Ecol 15:559–571
27. Hebel I, Haas R, Dounavi A (2006) Genetic variation of common ash (Fraxinus excelsior L.) populations from provenance regions in southern Germany by using nuclear and chloroplast microsatellites. Silvae Genetica 55:38–44
28. Hedrick PW (2005) A standardized genetic differentiation measure. Evolution 59:1633–1638
29. Herbert R, Samuel S, Pattison G (1999) Using local stock for planting native trees and shrubs. Forestry Commission Practice Note 8. Forestry Commission, Edinburgh, UK
30. Heuertz M, Hausman J-F, Tsvetkov I, Frascaria-Lacoste N, Vekemans X (2001) Assessment of genetic structure within and among Bulgarian populations of common as (Fraxinus excelsior L.). Mol Ecol 10:1615–1623
31. Heuertz M, Vekemans X, Hausman J-F, Palada M, Hardy OJ (2003) Estimating seed vs. pollen dispersal from spatial genetic structure in the common ash. Mol Ecol 12:2483–2495
32. Heuertz M, Fineschi S, Anzidei M et al (2004a) Chloroplast DNA variation and postglacial recolonization of common ash (Fraxinus excelsior L.) in Europe. Mol Ecol 13:3437–3452
33. Heuertz M, Hausman J-F, Hardy OJ, Vendramin GG, Frascaria-Lacoste N, Vekemans X (2004b) Nuclear microsatellites reveal contrasting patterns of genetic structure between western and southeastern European populations of the common ash (Fraxinus excelsior). Evolution 58:976–988
34. Howe HF, Smallwood J (1982) Ecology of seed dispersal. Annu Rev Ecol Syst 13:201–228
35. Janzen D (1970) Herbivores and the number of tree species in tropical forests. Am Nat 104:501–528
36. Jones N, Burley J (1973) Seed certification, provenance nomenclature and genetic history in forestry. Silvae Genetica 23:53–58
37. Kalinowski ST, Taper ML, Marshall TC (2007) Revising how the computer program Cervus accommodates genotyping error increases success in paternity assignment. Mol Ecol 16:1099–1106
38. Kowalski T (2006) Chalara fraxinea sp nov associated with dieback of ash (Fraxinus excelsior) in Poland. For Pathol 36:264–270
39. Lefort F, Brachet S, Frascaria-Lacoste N, Edwards KJ, Douglas GC (1999) Identification and characterization of microsatellite loci in ash (Fraxinus excelsior L.) and their conservation in the olive family. Mol Ecol Notes 8:1088–1090
40. Levine JM, Murrell DJ (2003) The community-level consequences of seed dispersal patterns. Annu Rev Ecol Evol Syst 34:549–574
41. Loiselle BA, Sork VL, Nason J, Graham C (1995) Spatial genetic structure of a tropical understorey shrub, Psychotria officinalis (Rubiaceae). Am J Bot 82:1420–1425
42. Marigo G, Peltier J-P, Girel J, Pautou G (2000) Success in the demographic expansion of Fraxinus excelsior L. Trees 15:1–13
43. Morand ME, Brachet S, Rossignol P, Dufour J, Frascaria-Lacoste N (2002) A generalised heterozygote deficiency assessed with microsatellites in French common ash populations. Mol Ecol 11:377–385
44. Nathan R, Muller-Landau HC (2000) Spatial patterns of seed dispersal, their determinants and consequences for recruitment. Trends Ecol Evol 15:278–285
45. Pautasso M, Aas G, Queloz V, Holdenreider O (2013) European as (Fraxinus excelsior) dieback—a conservation biology challenge. Biol Conserv 158:37–49
46. Powell W, Machray GC, Provan J (1996) Polymorphism revealed by simple sequence repeats. Trends Plant Sci 1:215–222
47. Provan J, Powell W, Hollingsworth PM (2001) Chloroplast microsatellites: new tools for studies in plant ecology and systematic. Trends Ecol Evol 16:142–147
48. Queloz V, Grüning CR, Berndt R, Kowalski T, Sieber TN, Holdenreider O (2011) Cryptic speciation in Hymenoscyphus albidus. For Pathol 41:133–142
49. Raymond M, Rousset F (1995) GenePop (version 1.2): population genetic software for exact tests and ecumenicism. J Hered 86:248–249
50. Rousset F (1997) Genetic differentiation and estimation of gene flow from F-statistics under isolation by distance. Genetics 145:1219–1228
51. Smouse PE, Sork VL, Scofield DG, Grivet D (2012) Using seedling and pericarp tissues to determine maternal parentage of dispersed valley oak recruits. J Hered 103:250–259
52. Sork VL, Smouse PE (2006) Genetic analysis of landscape connectivity in tree populations. Landsc Ecol 21:821–836
53. Steinitz O, Troupin D, Vendramin GG, Nathan R (2011) Genetic evidence for a Janzen-Connell recruitment pattern in reproductive offspring of Pinus halepensis trees. Mol Ecol 20:4152–4164
54. Sutherland BG, Belaj A, Nier S, Cottrell JE, Vaughan SP, Hubert J, Russell K (2010) Molecular biodiversity and population structure in common ash (Fraxinus excelsior L.) in Britain: implications for conservation. Mol Ecol 19:2196–2211
55. Thomasset M, Fernández-Manjarrés JF, Douglas GC, Bertolino P, Frascaria-Lacoste N, Hodkinson TR (2013) Assignment testing reveals multiple introduced source populations including potential ash hybrids (Fraxinus excelsior x F. angustifolia) in Ireland. Eur J For Res 132:195–209
56. Thomasset M, Hodkinson TR, Restoux G, Frascaria-Lacoste N, Douglas GC, Fernández-Manjarrés JF (2014) Thank you for not flowering: conservation genetics and gene flow analysis of native and non-native populations of Fraxinus (Oleaceae) in Ireland. Heredity 112:596–606
57. Vekemans X, Hardy OJ (2004) New insights from fine-scale spatial genetic structure analysis in plant populations. Mol Ecol 13:921–935
58. Wang BC, Smith TB (2002) Closing the seed dispersal loop. Trends Ecol Evol 17:379–385
59. Wardle P (1961) Biological flora of the British Isles: Fraxinus excelsior L. J Ecol 49:739–751
60. Webber J (1981) A natural biological control of Dutch elm disease. Nature 292:449–451
61. Weir BS, Cockerham CC (1984) Estimating F-statistics for the analysis of population structure. Evolution 38:1358–1370
62. Wolfe KH, Li W-H, Sharp PM (1987) Rates of nucleotide substitution vary greatly among plant mitochondrial, chloroplast and nuclear DNAs. Proc Natl Acad Sci U S A 84:9054–9058
63. Ziegenhagen B, Liepelt S, Kuhlenkamp V, Fladung M (2003) Molecular identification of individual oak and fir trees from maternal tissues of their fruits or seeds. Trees 17:345–350