Analysis of exosome purification methods using a model liposome system and tunable-resistive pulse sensing

Scientific Reports

Volumn 5, Issue , 2015, Pages

  Lane, Rebecca E ^a   Korbie, Darren ^a   Anderson, Will ^a   Vaidyanathan, Ramanathan ^a   Trau, Matt ^a  

^a UNIVERSITY OF QUEENSLAND   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

1,2-DIOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE; GLYCEROPHOSPHORYLCHOLINE; LIPOSOME;

ANALOGS AND DERIVATIVES; BIOLOGICAL MODEL; CHEMISTRY; EXOSOME; HUMAN; ISOLATION AND PURIFICATION; LIGHT; PARTICLE SIZE; PHYSIOLOGY; RADIATION SCATTERING; TUMOR CELL LINE; ULTRACENTRIFUGATION;

CELL LINE, TUMOR; EXOSOMES; GLYCERYLPHOSPHORYLCHOLINE; HUMANS; LIGHT; LIPOSOMES; MODELS, BIOLOGICAL; PARTICLE SIZE; SCATTERING, RADIATION; ULTRACENTRIFUGATION;

EID: 84928958602     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep07639     Document Type: Article

References (30)

1. Beaudoin, A. R.& Grondin, G. Shedding of vesicular material from the cell surface of eukaryotic cells: different cellular phenomena. Biochim Biophys Acta 1071, 203-219 (1991).
2. Valadi, H. et al. Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells. Nat Cell Biol 9, 654-659, doi:10.1038/ncb1596 (2007).
3. Skog, J. et al. Glioblastoma microvesicles transport RNA and proteins that promote tumour growth and provide diagnostic biomarkers. Nat Cell Biol 10, 1470-1476, doi:10.1038/ncb1800 (2008).
4. Orozco, A. F., Lewis, D. E. Flow cytometric analysis of circulatingmicroparticles in plasma. Cytometry A 77, 502-514, doi:10.1002/cyto.a.20886 (2010).
5. Gonzales, P. A. et al. Large-scale proteomics and phosphoproteomics of urinary exosomes. J Am Soc Nephrol 20, 363-379, doi:10.1681/ASN.2008040406 (2009).
6. Sharma, S., Gillespie, B. M., Palanisamy, V., Gimzewski, J. K. Quantitative nanostructural and single-molecule force spectroscopy biomolecular analysis of human-saliva-derived exosomes. Langmuir 27, 14394-14400, doi:10.1021/la2038763 (2011).
7. Lv, L. L. et al. MicroRNA-29c in urinary exosome/microvesicle as a biomarker of renal fibrosis. Am J Physiol Renal Physiol 305, F1220-1227, doi:10.1152/ajprenal.00148.2013 (2013).
8. Alvarez, S. et al. Urinary exosomes as a source of kidney dysfunction biomarker in renal transplantation. Transplant Proc 45, 3719-3723, doi:10.1016/j.transproceed.2013.08.079 (2013).
9. Duijvesz, D., Luider, T., Bangma, C. H., Jenster, G. Exosomes as biomarker treasure chests for prostate cancer. Eur Urol 59, 823-831, doi:10.1016/j.eururo.2010.12.031 (2011).
10. Thery, C., Amigorena, S., Raposo, G., Clayton, A. Isolation and characterizationof exosomes from cell culture supernatants and biological fluids. Curr Protoc Cell Biol 30, 3-22, doi: 10.1002/0471143030.cb0322s30 (2006).
11. Tauro, B. J. et al. Comparison of ultracentrifugation, density gradient separation, and immunoaffinity capture methods for isolating human colon cancer cell line LIM1863-derived exosomes. Methods 56, 293-304, doi:10.1016/j.ymeth.2012.01.002 (2012).
12. van der Pol, E. et al. Particle size distribution of exosomes and microvesicles determined by transmission electron microscopy, flow cytometry, nanoparticle tracking analysis, and resistive pulse sensing. J Thromb Haemost 12, 1182-1192, doi:10.1111/jth.12602 (2014).
13. Oosthuyzen, W. et al. Quantification of human urinary exosomes by nanoparticle tracking analysis. J Physiol 591, 5833-5842, doi:10.1113/jphysiol.2013.264069 (2013).
14. Kalra, H. et al. Comparative proteomics evaluation of plasma exosome isolation techniques and assessment of the stability of exosomes in normal human blood plasma. Proteomics 13, 3354-3364, doi:10.1002/pmic.201300282 (2013).
15. Dragovic, R. A. et al. Sizing and phenotyping of cellular vesicles using Nanoparticle Tracking Analysis. Nanomedicine 7, 780-788, doi:10.1016/j.nano.2011.04.003 (2011).
16. van der Pol, E. et al. Optical and non-optical methods for detection and characterization of microparticles and exosomes. J Thromb Haemost 8, 2596-2607, doi:10.1111/j.1538-7836.2010.04074.x (2010).
17. Alvarez-Erviti, L. et al.Delivery of siRNA to themouse brain by systemic injection of targeted exosomes. Nat Biotechnol 29, 341-345, doi:10.1038/nbt.1807 (2011).
18. Taylor, D. D., Zacharias, W., Gercel-Taylor, C. Exosome isolation for proteomic analyses and RNA profiling. Methods Mol Biol 728, 235-246, doi:10.1007/978-1-61779-068-3-15 (2011).
19. Rani, S. et al. Isolation of exosomes for subsequent mRNA, MicroRNA, and protein profiling. Methods Mol Biol 784, 181-195, doi:10.1007/978-1-61779-289-2-13 (2011).
20. Alvarez, M. L., Khosroheidari, M., Kanchi Ravi, R., DiStefano, J. K. Comparison of protein, microRNA, and mRNA yields using different methods of urinary exosome isolation for the discovery of kidney disease biomarkers. Kidney Int 82, 1024-1032, doi:10.1038/ki.2012.256 (2012).
21. Anderson, W., Kozak, D., Coleman, V. A., Jamting, A. K., Trau, M. A comparative study of submicron particle sizing platforms: accuracy, precision and resolution analysis of polydisperse particle size distributions. J Colloid Interface Sci 405, 322-330, doi:10.1016/j.jcis.2013.02.030 (2013).
22. Boing, A. N. et al. Active caspase-3 is removed from cells by release of caspase-3-enriched vesicles. Biochim Biophys Acta 1833, 1844-1852, doi:10.1016/j.bbamcr.2013.03.013 (2013).
23. de Vrij, J. et al. Quantification of nanosized extracellular membrane vesicles with scanning ion occlusion sensing. Nanomedicine (Lond) 8, 1443-1458, doi:10.2217/nnm.12.173 (2013).
24. Teng, H. et al. Suppression of inflammation by tumor-derived exosomes: a kind of natural liposome packaged with multifunctional proteins. J Liposome Res 22, 346-352, doi:10.3109/08982104.2012.710911 (2012).
25. Alanazi, F. K., Lu, D. R., Shakeel, F., Haq, N. Density gradient separation of carborane-containing liposome from low density lipoprotein and detection by inductively coupled plasma spectrometry. J Liposome Res 24, 53-58, doi:10.3109/08982104.2013.833224 (2014).
26. Versluis, A. J., Rump, E. T., Rensen, P. C., van Berkel, T. J., Bijsterbosch, M. K. Stable incorporation of a lipophilic daunorubicin prodrug into apolipoprotein Eexposing liposomes induces uptake of prodrug via low-density lipoprotein receptor in vivo. J Pharmacol Exp Ther 289, 1-7 (1999).
27. Abe, A., Miyanohara, A., Friedmann, T. Enhanced Gene Transfer with Fusogenic Liposomes Containing Vesicular Stomatitis Virus G Glycoprotein. J Virol 72, 6159-6163 (1998).
28. Roberts, G. S. et al. Tunable nano/micropores for particle detection and discrimination: scanning ion occlusion spectroscopy. Small 6, 2653-2658, doi:10.1002/smll.201001129 (2010).
29. DeBlois, R. W., Bean, C. P. Counting and Sizing of Submicron Particles by the Resistive Pulse Technique. Rev Sci Instrum 41, 909-916, doi:doi:http://dx.doi.org/10.1063/1.1684724 (1970).
30. Zhuang, X. et al. Treatment of brain inflammatory diseases by delivering exosome encapsulated anti-inflammatory drugs from the nasal region to the brain. Mol Ther 19, 1769-1779, doi:10.1038/mt.2011.164 (2011).