Automated video-based analysis of contractility and calcium flux in human-induced pluripotent stem cell-derived cardiomyocytes cultured over different spatial scales

Tissue Engineering - Part C: Methods

Volumn 21, Issue 5, 2015, Pages 467-479

  Huebsch, Nathaniel ^a   Loskill, Peter ^b,c   Mandegar, Mohammad A ^a   Marks, Natalie C ^b   Sheehan, Alice S ^a   Ma, Zhen ^b   Mathur, Anurag ^b,c   Nguyen, Trieu N ^a   Yoo, Jennie C ^a   Judge, Luke M ^a   Spencer, C Ian ^a   Chukka, Anand C ^a   Russell, Caitlin R ^a   So, Po Lin ^a   Conklin, Bruce R ^a,d   Healy, Kevin E ^b,c  

^a GLADSTONE INSTITUTE OF CARDIOVASCULAR DISEASE   (United States)

^b University of California at Berkeley   (United States)

^c Lawrence Berkeley National Laboratory   (United States)

^d UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CALCIUM; CELL ENGINEERING; CYTOLOGY; OPEN SOURCE SOFTWARE; OPEN SYSTEMS; SIGNAL TO NOISE RATIO; TISSUE ENGINEERING;

AUTOMATED METHODS; CALCIUM INDICATOR; HUMAN-INDUCED PLURIPOTENT STEM CELLS; MICROENVIRONMENTS; RELATIVE PERFORMANCE; SIGNAL TO NOISE; USER INDEPENDENTS; VIDEO MICROSCOPY;

STEM CELLS;

CALCIUM; INDICATOR; ISOPRENALINE;

ALGORITHM; ARTICLE; AUTOMATION; CALCIUM TRANSPORT; CAMERA; CELL CULTURE; COMPUTER PROGRAM; DRUG RESPONSE; ELECTROPHYSIOLOGY; EXCITATION CONTRACTION COUPLING; HEART CELL CULTURE; HEART MUSCLE CELL; HUMAN; HUMAN CELL; MICROENVIRONMENT; MUSCLE CONTRACTILITY; NOISE; OPTIC FLOW; PATCH CLAMP TECHNIQUE; PLURIPOTENT STEM CELL; PRIORITY JOURNAL; TISSUE ENGINEERING; VIDEO MICROSCOPY; VIDEORECORDING; AUTOMATED PATTERN RECOGNITION; CARDIAC MUSCLE CELL; CELL DIFFERENTIATION; CYTOLOGY; HEART CONTRACTION; IMAGE PROCESSING; INDUCED PLURIPOTENT STEM CELL; METABOLISM; PROCEDURES; SIGNAL NOISE RATIO; SIGNAL TRANSDUCTION; SOFTWARE;

IPS;

ALGORITHMS; CALCIUM; CELL DIFFERENTIATION; CELLS, CULTURED; HUMANS; IMAGE PROCESSING, COMPUTER-ASSISTED; INDUCED PLURIPOTENT STEM CELLS; MICROSCOPY, VIDEO; MYOCARDIAL CONTRACTION; MYOCYTES, CARDIAC; PATCH-CLAMP TECHNIQUES; PATTERN RECOGNITION, AUTOMATED; SIGNAL TRANSDUCTION; SIGNAL-TO-NOISE RATIO; SOFTWARE;

EID: 84928949091     PISSN: 19373384     EISSN: 19373392     Source Type: Journal    
DOI: 10.1089/ten.tec.2014.0283     Document Type: Article

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