A therapy-grade protocol for differentiation of pluripotent stem cells into mesenchymal stem cells using platelet lysate as supplement

Stem Cell Research and Therapy

Volumn 6, Issue 1, 2015, Pages

  Luzzani, Carlos ^a   Neiman, Gabriel ^a   Garate, Ximena ^a   Questa, María ^a   Solari, Claudia ^b   Fernandez Espinosa, Darío ^a   García, Marcela ^c   Errecalde, Ana Lía ^c   Guberman, Alejandra ^b,d   Scassa, María Elida ^a   Sevlever, Gustavo Emilio ^a   Romorini, Leonardo ^a,d   Miriuka, Santiago Gabriel ^a,c,d  

^a Laboratorio de Neuroimágenes   (Argentina)

^b UNIVERSIDAD DE BUENOS AIRES   (Argentina)

^c FACULTAD DE CIENCIAS MÉDICAS   (Argentina)

^d Facultad de Ciencias Naturales e Instituto M Lillo   (Argentina)

Author keywords

[No Author keywords available]

Indexed keywords

5' NUCLEOTIDASE; ACTIVATED LEUKOCYTE CELL ADHESION MOLECULE; CD271 ANTIGEN; CELL SURFACE MARKER; ENDOGLIN; INTEGRIN; LYMPHOCYTE ANTIGEN; OCTAMER TRANSCRIPTION FACTOR 4; SCLEROPROTEIN; THY 1 ANTIGEN; UNCLASSIFIED DRUG; MEMBRANE ANTIGEN; SIGNAL PEPTIDE;

ANTIGEN EXPRESSION; ARTICLE; CELL DIFFERENTIATION; CELL LINEAGE; CELL LYSATE; CELL STIMULATION; CONTROLLED STUDY; CULTURE MEDIUM; DNA METHYLATION; EMBRYO; FLOW CYTOMETRY; HUMAN; HUMAN CELL; IMMUNOMODULATION; LYMPHOCYTE; MESENCHYMAL STEM CELL; PERIPHERAL BLOOD MONONUCLEAR CELL; PLATELET LYSATE; PLURIPOTENT STEM CELL; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN ANALYSIS; PROTEIN EXPRESSION; STEM CELL EXPANSION; UMBILICAL CORD; CELL CULTURE; CYTOLOGY; DRUG EFFECTS; FLUORESCENCE MICROSCOPY; HUMAN EMBRYONIC STEM CELL; MESENCHYMAL STROMA CELL; METABOLISM; PHENOTYPE; THROMBOCYTE;

BOVINAE;

ANTIGENS, SURFACE; BLOOD PLATELETS; CELL DIFFERENTIATION; CELLS, CULTURED; DNA METHYLATION; HUMAN EMBRYONIC STEM CELLS; HUMANS; INTERCELLULAR SIGNALING PEPTIDES AND PROTEINS; MESENCHYMAL STROMAL CELLS; MICROSCOPY, FLUORESCENCE; PHENOTYPE; PLURIPOTENT STEM CELLS; PROMOTER REGIONS, GENETIC;

EID: 84928742351     PISSN: None     EISSN: 17576512     Source Type: Journal    
DOI: 10.1186/scrt540     Document Type: Article

References (45)

1. Kern S, Eichler H, Stoeve J, Klüter H, Bieback K: Comparative analysis of mesenchymal stem cells from bone marrow, umbilical cord blood, or adipose tissue. Stem Cells. 2006, 24:1294-301. 10.1634/stemcells.2005-0342
2. Izadpanah R, Kaushal D, Kriedt C, Tsien F, Patel B, Dufour J, et al.: Long-term in vitro expansion alters the biology of adult mesenchymal stem cells. Cancer Res. 2008, 68:4229-38. 10.1158/0008-5472.CAN-07-5272
3. Sensebé L, Bourin P, Tarte K: Good manufacturing practices production of mesenchymal stem/stromal cells. Hum Gene Ther. 2011, 22:19-26. 10.1089/hum.2010.197
4. Bieback K: Platelet lysate as replacement for fetal bovine serum in mesenchymal stromal cell cultures. Transfus Med Hemother. 2013, 40:326-35. 10.1159/000354061
5. Schallmoser K, Strunk D: Generation of a pool of human platelet lysate and efficient use in cell culture. Methods Mol Biol. 2013, 946:349-62. 10.1007/978-1-62703-128-8-22
6. Rauch C, Feifel E, Amann E-M, Spötl HP, Schennach H, Pfaller W, et al.: Alternatives to the use of fetal bovine serum: human platelet lysates as a serum substitute in cell culture media. ALTEX. 2011, 28:305-16. 10.14573/altex.2011.4.305
7. Iudicone P, Fioravanti D, Bonanno G, Miceli M, Lavorino C, Totta P, et al.: Pathogen-free, plasma-poor platelet lysate and expansion of human mesenchymal stem cells. J Transl Med. 2014, 12:28. 10.1186/1479-5876-12-28
8. Kinzebach S, Dietz L, Klüter H, Thierse H-J, Bieback K: Functional and differential proteomic analyses to identify platelet derived factors affecting ex vivo expansion of mesenchymal stromal cells. BMC Cell Biol. 2013, 14:48. 10.1186/1471-2121-14-48
9. Flemming A, Schallmoser K, Strunk D, Stolk M, Volk H-D, Seifert M: Immunomodulative efficacy of bone marrow-derived mesenchymal stem cells cultured in human platelet lysate. J Clin Immunol. 2011, 31:1143-56. 10.1007/s10875-011-9581-z
10. Lucchini G, Introna M, Dander E, Rovelli A, Balduzzi A, Bonanomi S, et al.: Platelet-lysate-expanded mesenchymal stromal cells as a salvage therapy for severe resistant graft-versus-host disease in a pediatric population. Biol Blood Marrow Transplant. 2010, 16:1293-301. 10.1016/j.bbmt.2010.03.017
11. Hematti P: Human embryonic stem cell-derived mesenchymal progenitors: an overview. Methods Mol Biol. 2011, 690:163-74. 10.1007/978-1-60761-962-8-11
12. Hematti P: Human embryonic stem cell-derived mesenchymal stromal cells. Transfusion. 2011, 51:138S-44.
13. Jung Y, Bauer G, Nolta JA: Concise review: Induced pluripotent stem cell-derived mesenchymal stem cells: progress toward safe clinical products. Stem Cells. 2012, 30:42-7. 10.1002/stem.727
14. Barberi T, Willis LM, Socci ND, Studer L: Derivation of multipotent mesenchymal precursors from human embryonic stem cells. PLoS Med. 2005, 2:e161. 10.1371/journal.pmed.0020161
15. Olivier EN, Rybicki AC, Bouhassira EE: Differentiation of human embryonic stem cells into bipotent mesenchymal stem cells. Stem Cells. 2006, 24:1914-22. 10.1634/stemcells.2005-0648
16. Lian Q, Lye E, Suan Yeo K: Khia Way Tan E, Salto-Tellez M, Liu TM, et al. Derivation of clinically compliant MSCs from CD105+, CD24- differentiated human ESCs. Stem Cells. 2007, 25:425-36. 10.1634/stemcells.2006-0420
17. Trivedi P, Hematti P: Derivation and immunological characterization of mesenchymal stromal cells from human embryonic stem cells. Exp Hematol. 2008, 36:350-9.
18. Hwang NS, Varghese S, Lee HJ, Zhang Z, Ye Z, Bae J, et al.: In vivo commitment and functional tissue regeneration using human embryonic stem cell-derived mesenchymal cells. Proc Natl Acad Sci U S A. 2008, 105:20641-6. 10.1073/pnas.0809680106
19. Boyd NL, Robbins KR, Dhara SK, West FD, Stice SL: Human embryonic stem cell-derived mesoderm-like epithelium transitions to mesenchymal progenitor cells. Tissue Eng Part A. 2009, 15:1897-907. 10.1089/ten.tea.2008.0351
20. Lee EJ, Lee H-N, Kang H-J, Kim K-H, Hur J, Cho H-J, et al.: Novel embryoid body-based method to derive mesenchymal stem cells from human embryonic stem cells. Tissue Eng Part A. 2010, 16:705-15.
21. Sánchez L, Gutierrez-Aranda I, Ligero G, Rubio R, Muñoz-López M, García-Pérez JL, et al.: Enrichment of human ESC-derived multipotent mesenchymal stem cells with immunosuppressive and anti-inflammatory properties capable to protect against experimental inflammatory bowel disease. Stem Cells. 2011, 29:251-62. 10.1002/stem.569
22. Harkness L, Mahmood A, Ditzel N, Abdallah BM, Nygaard JV, Kassem M: Selective isolation and differentiation of a stromal population of human embryonic stem cells with osteogenic potential. Bone. 2011, 48:231-41. 10.1016/j.bone.2010.09.023
23. Diederichs S, Tuan RS: Functional comparison of human-induced pluripotent stem cell-derived mesenchymal cells and bone marrow-derived mesenchymal stromal cells from the same donor. Stem Cells Dev. 2014, 23:1594-610. 10.1089/scd.2013.0477
24. Yen BL, Chang CJ, Liu K-J, Chen YC, Hu H-I, Bai C-H, et al.: Brief report-human embryonic stem cell-derived mesenchymal progenitors possess strong immunosuppressive effects toward natural killer cells as well as T lymphocytes. Stem Cells. 2009, 27:451-6. 10.1634/stemcells.2008-0390
25. Giuliani M, Oudrhiri N, Noman ZM, Vernochet A, Chouaib S, Azzarone B, et al.: Human mesenchymal stem cells derived from induced pluripotent stem cells down-regulate NK-cell cytolytic machinery. Blood. 2011, 118:3254-62. 10.1182/blood-2010-12-325324
26. Kimbrel EA, Kouris NA, Yavanian GJ, Chu J, Qin Y, Chan A, et al.: Mesenchymal stem cell population derived from human pluripotent stem cells displays potent immunomodulatory and therapeutic properties. Stem Cells Dev. 2014, 23:1611-24. 10.1089/scd.2013.0554
27. Schnabel LV, Abratte CM, Schimenti JC, Felippe MJB, Cassano JM, Southard TL, et al.: Induced pluripotent stem cells have similar immunogenic and more potent immunomodulatory properties compared with bone marrow-derived stromal cells in vitro. Regen Med. 2014, 9:621-35. 10.2217/rme.14.29
28. Sommer CA, Stadtfeld M, Murphy GJ, Hochedlinger K, Kotton DN, Mostovslasky G: Induced pluripotent stem cell generation using a single lentiviral stem cell cassette. Stem Cells. 2009, 27:543-9. 10.1634/stemcells.2008-1075
29. Copland IB, Garcia MA, Waller EK, Roback JD, Galipeau J: The effect of platelet lysate fibrinogen on the functionality of MSCs in immunotherapy. Biomaterials. 2013, 34:7840-50. 10.1016/j.biomaterials.2013.06.050
30. Quah BJC, Warren HS, Parish CR: Monitoring lymphocyte proliferation in vitro and in vivo with the intracellular fluorescent dye carboxyfluorescein diacetate succinimidyl ester. Nat Protoc. 2007, 2:2049-56. 10.1038/nprot.2007.296
31. Freberg CT, Dahl JA, Timoskainen S, Collas P: Epigenetic reprogramming of OCT4 and NANOG regulatory regions by embryonal carcinoma cell extract. Mol Biol Cell. 2007, 18:1543-53. 10.1091/mbc.E07-01-0029
32. Lohmann M, Walenda G, Hemeda H, Joussen S, Drescher W, Jockenhoevel S, et al.: Donor age of human platelet lysate affects proliferation and differentiation of mesenchymal stem cells. PLoS ONE. 2012, 7:e37839. 10.1371/journal.pone.0037839
33. Doucet C, Ernou I, Zhang Y, Llense J-R, Begot L, Holy X, et al.: Platelet lysates promote mesenchymal stem cell expansion: a safety substitute for animal serum in cell-based therapy applications. J Cell Physiol. 2005, 205:228-36. 10.1002/jcp.20391
34. Liu Y, Goldberg AJ, Dennis JE, Gronowicz GA, Kuhn LT: One-step derivation of mesenchymal stem cell (MSC)-like cells from human pluripotent stem cells on a fibrillar collagen coating. PLoS ONE. 2012, 7:e33225. 10.1371/journal.pone.0033225
35. Wang X, Dai J: Concise review: isoforms of OCT4 contribute to the confusing diversity in stem cell biology. Stem Cells. 2010, 28:885-93.
36. Zangrossi S, Marabese M, Broggini M, Giordano R, D'Erasmo M, Montelatici E, et al.: Oct-4 expression in adult human differentiated cells challenges its role as a pure stem cell marker. Stem Cells. 2007, 25:1675-80. 10.1634/stemcells.2006-0611
37. Mueller T, Luetzkendorf J, Nerger K, Schmoll HJ, Mueller LP: Analysis of OCT4 expression in an extended panel of human tumor cell lines from multiple entities and in human mesenchymal stem cells. Cell Mol Life Sci. 2009, 66:495-503. 10.1007/s00018-008-8623-z
38. Schallmoser K, Bartmann C, Rohde E, Reinisch A, Kashofer K, Stadelmeyer E, et al.: Human platelet lysate can replace fetal bovine serum for clinical-scale expansion of functional mesenchymal stromal cells. Transfusion. 2007, 47:1436-46. 10.1111/j.1537-2995.2007.01220.x
39. Capelli C, Domenghini M, Borleri G, Bellavita P, Poma R, Carobbio A, et al.: Human platelet lysate allows expansion and clinical grade production of mesenchymal stromal cells from small samples of bone marrow aspirates or marrow filter washouts. Bone Marrow Transplant. 2007, 40:785-91. 10.1038/sj.bmt.1705798
40. Xu C, Inokuma MS, Denham J, Golds K, Kundu P, Gold JD, et al.: Feeder-free growth of undifferentiated human embryonic stem cells. Nat Biotechnol. 2001, 19:971-4. 10.1038/nbt1001-971
41. Rosler ES, Fisk GJ, Ares X, Irving J, Miura T, Rao MS, et al.: Long-term culture of human embryonic stem cells in feeder-free conditions. Dev Dyn. 2004, 229:259-74. 10.1002/dvdy.10430
42. Stojkovic P, Lako M, Stewart R, Przyborski S, Armstrong L, Evans J, et al.: An autogeneic feeder cell system that efficiently supports growth of undifferentiated human embryonic stem cells. Stem Cells. 2005, 23:306-14. 10.1634/stemcells.2004-0137
43. Frobel J, Hemeda H, Lenz M, Abagnale G, Joussen S, Denecke B, et al.: Epigenetic rejuvenation of mesenchymal stromal cells derived from induced pluripotent stem cells. Stem Cell Rep. 2014, 3:414-22. 10.1016/j.stemcr.2014.07.003
44. Valencic E, Piscianz E, Andolina M, Ventura A, Tommasini A: The immunosuppressive effect of Wharton's jelly stromal cells depends on the timing of their licensing and on lymphocyte activation. Cytotherapy. 2010, 12:154-60. 10.3109/14653240903493417
45. Wang X, Kimbrel EA, Ijichi K, Paul D, Lazorchak AS, Chu J, et al.: Human ESC-derived MSCs outperform bone marrow MSCs in the treatment of an EAE model of multiple sclerosis. Stem Cell Rep. 2014, 3:115-30. 10.1016/j.stemcr.2014.04.020