A New-Generation Fluorescent-Based Metal Sensor – iLOV Protein

Journal of Microbiology and Biotechnology

Volumn 25, Issue 4, 2015, Pages 503-510

  Ravikumar, Yuvaraj ^a   Nadarajan, Saravanan Prabhu ^b   Lee, Chong Soon ^a   Rhee, Jin Kyu ^c   Yun, Hyungdon ^b  

^a Yeungnam University   (South Korea)

^b Konkuk University   (South Korea)

^c Ewha Womans University   (South Korea)

Author keywords

Copper; Fluorescence quenching; iLOV protein; Metal sensor

Indexed keywords

COPPER ION; EDETIC ACID; FLAVINE MONONUCLEOTIDE; FLUORESCENT DYE; ILOV PROTEIN; METAL SENSOR; UNCLASSIFIED DRUG; COPPER; METAL; PROTEIN; PROTEIN BINDING; RECOMBINANT PROTEIN;

ARTICLE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; DISSOCIATION CONSTANT; ESCHERICHIA COLI; FEASIBILITY STUDY; FLUORESCENCE ANALYSIS; METAL BINDING; NONHUMAN; PH; PHOTOCHEMICAL QUENCHING; PROTEIN ANALYSIS; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN PURIFICATION; SPECTROSCOPY; BINDING SITE; CHEMISTRY; FLUORESCENCE; GENETIC PROCEDURES; GENETICS; KINETICS; METABOLISM; PHOTORECEPTOR CELL; PHYSIOLOGY;

BINDING SITES; BIOSENSING TECHNIQUES; COPPER; EDETIC ACID; FLUORESCENCE; HYDROGEN-ION CONCENTRATION; KINETICS; METALS; PHOTORECEPTOR CELLS; PROTEIN BINDING; PROTEIN INTERACTION DOMAINS AND MOTIFS; PROTEINS; RECOMBINANT PROTEINS;

EID: 84928116320     PISSN: 10177825     EISSN: 17388872     Source Type: Journal    
DOI: 10.4014/jmb.1409.09035     Document Type: Article

References (23)

1. Ayyadurai N, Prabhu NS, Deepankumar K, Jang YJ, Chitrapriya N, Song E, et al. Bioconjugation of l-3, 4-dihydroxyphenylalanine-containing protein with a polysaccharide. Bioconjug. Chem. 22: 551-555.
2. Ayyadurai N, Saravanan Prabhu N, Deepankumar K, Lee SG, Jeong HH, Lee CS, Yun H. 2011. Development of a selective, sensitive, and reversible biosensor by the genetic incorporation of a metal-binding site into green fluorescent protein. Angew. Chem. Int. Ed. Engl. 50: 6534-6537.
3. Balint EE, Petres J, Szabo M, Orban CK, Szilagyi L, Abraham B. 2013. Fluorescence of a histidine-modified enhanced green fluorescent protein (EGFP) effectively quenched by copper (II) ions. J. Fluoresc. 23: 273-281.
4. Chapman S, Faulkner C, Kaiserli E, Garcia-Mata C, Savenkov EI, Roberts AG, et al. 2008. The photoreversible fluorescent protein iLOV outperforms GFP as a reporter of plant virus infection. Proc. Natl. Acad. Sci. USA 105: 20038-20043.
5. Christie JM, Hitomi K, Arvai AS, Hartfield KA, Mettlen M, Pratt AJ, et al. Structural tuning of the fluorescent protein iLOV for improved photostability. J. Biol. Chem. 287: 22295-22304.
6. Chu J, Haynes RD, Corbel SY, Li P, Gonzalez-Gonzalez E, Burg JS, et al. Non-invasive intravital imaging of cellular differentiation with a bright red-excitable fluorescent protein. Nat. Methods 11: 572-578.
7. Davis SJ, Vierstra RD. 1998. Soluble, highly fluorescent variants of green fluorescent protein (GFP) for use in higher plants. Plant Mol. Biol. 36: 521-528.
8. Drepper T, Eggert T, Circolone F, Heck A, Krauss U, Guterl JK, et al. 2007. Reporter proteins for in vivo fluorescence without oxygen. Nat. Biotechnol. 25: 443-445.
9. Eli P, Chakrabartty A. 2006. Variants of DsRed fluorescent protein: development of a copper sensor. Protein Sci. 15: 2442-2447.
10. Giepmans BN, Adams SR, Ellisman MH, Tsien RY. 2006. The fluorescent toolbox for assessing protein location and function. Science 312: 217-224.
11. Isarankura-Na-Ayudhya C, Tantimongcolwat T, Galla HJ, Prachayasittikul V. 2010. Fluorescent protein-based optical biosensor for copper ion quantitation. Biol. Trace Elem. Res. 134: 352-363.
12. Kaler SG. 2011. ATP7A-related copper transport diseases - emerging concepts and future trends. Nat. Rev. Neurol. 7: 15-29.
13. Kimura S, Noda T, Yoshimori T. 2007. Dissection of the autophagosome maturation process by a novel reporter protein, tandem fluorescent-tagged LC3. Autophagy 3: 452-460.
14. Llopis J, McCaffery JM, Miyawaki A, Farquhar MG, Tsien RY. 1998. Measurement of cytosolic, mitochondrial, and golgi pH in single living cells with green fluorescent proteins. Proc. Natl. Acad. Sci. USA 95: 6803-6808.
15. Mukherjee A, Schroeder CM. 2014. Flavin-based fluorescent proteins: emerging paradigms in biological imaging. Curr. Opin. Biotechnol. 31C: 16-23.
16. Mukherjee A, Walker J, Weyant KB, Schroeder CM. 2013. Characterization of flavin-based fluorescent proteins: an emerging class of fluorescent reporters. PLoS One 8: e64753.
17. Rahimi Y, Goulding A, Shrestha S, Mirpuri S, Deo SK. 2008. Mechanism of copper induced fluorescence quenching of red fluorescent protein, DsRed. Biochem. Biophys. Res. Commun. 370: 57-61.
18. Rahimi Y, Shrestha S, Banerjee T, D eo S K. 2007. C op p er sensing based on the far-red fluorescent protein, HcRed, from Heteractis crispa. Anal. Biochem. 370: 60-67.
19. Sumner JP, Westerberg NM, Stoddard AK, Hurst TK, Cramer M, Thompson RB, et al. 2006. DsRed as a highly sensitive, selective, and reversible fluorescence-based biosensor for both Cu+ and Cu2+ ions. Biosens. Bioelectron. 21: 1302-1308.
20. Tansila N, Tantimongcolwat T, Isarankura-Na-Ayudhya C, Nantasenamat C, Prachayasittikul V. 2007. Rational design of analyte channels of the green fluorescent protein for biosensor applications. Int. J. Biol. Sci. 3: 463-470.
21. Tsien RY. 1998. The green fluorescent protein. Annu. Rev. Biochem. 67: 509-544.
22. Verma N, Singh M. 2005. Biosensors for heavy metals. Biometals 18: 121-129.
23. Vinkenborg JL, Nicolson TJ, Bellomo EA, Koay MS, Rutter GA, Merkx M. 2009. Genetically encoded fret sensors to monitor intracellular Zn2+ homeostasis. Nat. Methods 6: 737-740.