In vitro models of the blood-brain barrier

Methods in Molecular Biology

Volumn 1135, Issue , 2014, Pages 415-437

  Czupalla, Cathrin J ^a   Liebner, Stefan ^a   Devraj, Kavi ^a  

^a GOETHE UNIVERSITY MEDICAL SCHOOL   (Germany)

Author keywords

Astrocytes; Blood brain barrier; cellZscope ; Coculture; In vitro model; Microvascular brain endothelial cells; Permeability; TEER

Indexed keywords

ANIMAL CELL; ANIMAL CELL CULTURE; ARTICLE; ASTROCYTE; BLOOD BRAIN BARRIER; CELL ADHESION; CELL GROWTH; CELL ISOLATION; CELL MEMBRANE PERMEABILITY; COCULTURE; CULTURE MEDIUM; ELECTRIC RESISTANCE; ENDOTHELIUM CELL; HUMAN; HUMAN CELL; IMMUNOCYTOCHEMISTRY; IN VITRO STUDY; MICROVASCULAR ENDOTHELIAL CELL; MICROVASCULATURE; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; BLOOD VESSEL PERMEABILITY; BRAIN BLOOD VESSEL; CELL MEMBRANE; CENTRAL NERVOUS SYSTEM; IMPEDANCE; MEASUREMENT; MONOLAYER CULTURE; REPRODUCIBILITY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TIGHT JUNCTION; TRANS ENDOTHELIAL ELECTRICAL RESISTANCE; WESTERN BLOTTING;

MURINAE;

ANIMALS; ASTROCYTES; BLOOD-BRAIN BARRIER; BRAIN; CAPILLARY PERMEABILITY; CELL CULTURE TECHNIQUES; CELL LINE; COCULTURE TECHNIQUES; ELECTRIC IMPEDANCE; ENDOTHELIAL CELLS; GENE EXPRESSION PROFILING; HUMANS; MICE; MICE, INBRED C57BL; MICROVESSELS; REAL-TIME POLYMERASE CHAIN REACTION; RNA, MESSENGER; TIGHT JUNCTIONS; TISSUE FIXATION;

EID: 84927934861     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-320-7_34     Document Type: Article

References (24)

1. Abbott NJ, Patabendige AA, Dolman DE, Yusof SR, Begley DJ (2010) Structure and function of the blood-brain barrier. Neurobiol Dis 37:13-25
2. Pardridge WM (2003) Molecular biology of the blood-brain barrier. Methods Mol Med 89:385-399
3. Pardridge WM (2005) The blood-brain barrier: bottleneck in brain drug development. NeuroRx 2:3-14
4. Neuwelt E, Abbott NJ, Abrey L, Banks WA, Blakley B, Davis T, Engelhardt B, Grammas P, Nedergaard M, Nutt J, Pardridge W, Rosenberg GA, Smith Q, Drewes LR (2008) Strategies to advance translational research into brain barriers. Lancet Neurol 7:84-96
5. Deli MA, Abraham CS, Kataoka Y, Niwa M (2005) Permeability studies on in vitro bloodbrain barrier models: physiology, pathology, and pharmacology. Cell Mol Neurobiol 25:59-127
6. Lippmann ES, Azarin SM, Kay JE, Nessler RA, Wilson HK, Al-Ahmad A, Palecek SP, Shusta EV (2012) Derivation of blood-brain barrier endothelial cells from human pluripotent stem cells. Nat Biotechnol 30:783-791
7. Lyck R, Ruderisch N, Moll AG, Steiner O, Cohen CD, Engelhardt B, Makrides V, Verrey F (2009) Culture-induced changes in bloodbrain barrier transcriptome: implications for amino-acid transporters in vivo. J Cereb Blood Flow Metab 29:1491-1502
8. Calabria AR, Shusta EV (2008) A genomic comparison of in vivo and in vitro brain microvascular endothelial cells. J Cereb Blood Flow Metab 28:135-148
9. Tilling T, Korte D, Hoheisel D, Galla HJ (1998) Basement membrane proteins influence brain capillary endothelial barrier function in vitro. J Neurochem 71:1151-1157
10. Benson K, Cramer S, Galla HJ (2013) Impedance-based cell monitoring: barrier properties and beyond. Fluids Barriers CNS 10:5
11. Gunzel D, Zakrzewski SS, Schmid T, Pangalos M, Wiedenhoeft J, Blasse C, Ozboda C, Krug SM (2012) From TER to trans- and paracellular resistance: lessons from impedance spectroscopy. Ann N Y Acad Sci 1257:142-151
12. Nitta T, Hata M, Gotoh S, Seo Y, Sasaki H, Hashimoto N, Furuse M, Tsukita S (2003) Size-selective loosening of the blood-brain barrier in claudin-5-deficient mice. J Cell Biol 161:653-660
13. Van Itallie C, Rahner C, Anderson JM (2001) Regulated expression of claudin-4 decreases paracellular conductance through a selective decrease in sodium permeability. J Clin Invest 107:1319-1327
14. Van Itallie CM, Anderson JM (2011) Measuring size-dependent permeability of the tight junction using PEG profiling. Methods Mol Biol 762:1-11
15. Liebner S, Corada M, Bangsow T, Babbage J, Taddei A, Czupalla CJ, Reis M, Felici A, Wolburg H, Fruttiger M, Taketo MM, von Melchner H, Plate KH, Gerhardt H, Dejana E (2008) Wnt/beta-catenin signaling controls development of the blood-brain barrier. J Cell Biol 183:409-417
16. Rohnelt RK, Hoch G, Reiss Y, Engelhardt B (1997) Immunosurveillance modelled in vitro: naive and memory T cells spontaneously migrate across unstimulated microvascular endothelium. Int Immunol 9:435-450
17. Weksler BB, Subileau EA, Perriere N, Charneau P, Holloway K, Leveque M, Tricoire-Leignel H, Nicotra A, Bourdoulous S, Turowski P, Male DK, Roux F, Greenwood J, Romero IA, Couraud PO (2005) Blood-brain barrier- specific properties of a human adult brain endothelial cell line. FASEB J 19:1872-1874
18. Uliasz TF, Hamby ME, Jackman NA, Hewett JA, Hewett SJ (2012) Generation of primary astrocyte cultures devoid of contaminating microglia. Methods Mol Biol 814:61-79
19. Liebner S, Czupalla CJ, Wolburg H (2011) Current concepts of blood-brain barrier development. Int J Dev Biol 55:467-476
20. Crone C, Olesen SP (1982) Electrical resistance of brain microvascular endothelium. Brain Res 241:49-55
21. Li G, Simon MJ, Cancel LM, Shi ZD, Ji X, Tarbell JM, Morrison B 3rd, Fu BM (2010) Permeability of endothelial and astrocyte cocultures: in vitro blood-brain barrier models for drug delivery studies. Ann Biomed Eng 38: 2499-2511
22. Antonetti DA, Wolpert EB (2003) Isolation and characterization of retinal endothelial cells. Methods Mol Med 89:365-374
23. Kroll S, El-Gindi J, Thanabalasundaram G, Panpumthong P, Schrot S, Hartmann C, Galla HJ (2009) Control of the blood-brain barrier by glucocorticoids and the cells of the neurovascular unit. Ann N Y Acad Sci 1165:228-239
24. Zhang Y, Li CS, Ye Y, Johnson K, Poe J, Johnson S, Bobrowski W, Garrido R, Madhu C (2006) Porcine brain microvessel endothelial cells as an in vitro model to predict in vivo blood-brain barrier permeability. Drug Metab Dispos 34:1935-1943