Mesenchymal stem cells suppress fibroblast proliferation and reduce skin fibrosis through a TGF-β3-dependent activation

International Journal of Lower Extremity Wounds

Volumn 14, Issue 1, 2015, Pages 50-62

  Wu, Yan ^a,b   Peng, Yan ^c   Gao, Dongyun ^d   Feng, Changjiang ^e   Yuan, Xiaohuan ^a   Li, Houzhong ^a   Wang, Ying ^a   Yang, Lan ^a   Huang, Sha ^b   Fu, Xiaobing ^b  

^a MUDANJIANG MEDICAL UNIVERSITY   (China)

^b CHINESE PLA GENERAL HOSPITAL   (China)

^c UNIVERSITY OF HONG KONG   (Hong Kong)

^d Dongtai People's Hospital   (China)

^e PEKING UNIVERSITY PEOPLE S HOSPITAL   (China)

Author keywords

mesenchymal stem cells; paracrine; skin fibrosis; transforming growth factor beta

Indexed keywords

ALPHA SMOOTH MUSCLE ACTIN; COLLAGEN; TRANSFORMING GROWTH FACTOR BETA3; RNA;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CELL DIFFERENTIATION; CELL PROLIFERATION; CELL VIABILITY; CONTROLLED STUDY; EXTRACELLULAR MATRIX; FEMALE; HISTOPATHOLOGY; HUMAN; HUMAN CELL; IMMUNOHISTOCHEMISTRY; IN VITRO STUDY; KELOID; MESENCHYMAL STEM CELL; MOUSE; MYOFIBROBLAST; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN ISOLATION; SKIN FIBROBLAST; SKIN FIBROSIS; TISSUE SECTION; ADULT; ANIMAL; BIOSYNTHESIS; C57BL MOUSE; CELL CULTURE; CYTOLOGY; DISEASE MODEL; FIBROBLAST; FIBROSIS; GENE EXPRESSION REGULATION; GENETICS; MALE; MESENCHYMAL STEM CELL TRANSPLANTATION; MESENCHYMAL STROMA CELL; METABOLISM; PATHOLOGY; REAL TIME POLYMERASE CHAIN REACTION; SKIN DISEASES; WESTERN BLOTTING; YOUNG ADULT;

ADULT; ANIMALS; BLOTTING, WESTERN; CELL PROLIFERATION; CELLS, CULTURED; DISEASE MODELS, ANIMAL; FEMALE; FIBROBLASTS; FIBROSIS; GENE EXPRESSION REGULATION; HUMANS; MALE; MESENCHYMAL STEM CELL TRANSPLANTATION; MESENCHYMAL STROMAL CELLS; MICE; MICE, INBRED C57BL; REAL-TIME POLYMERASE CHAIN REACTION; RNA; SKIN DISEASES; TRANSFORMING GROWTH FACTOR BETA3; YOUNG ADULT;

EID: 84927520441     PISSN: 15347346     EISSN: 15526941     Source Type: Journal    
DOI: 10.1177/1534734614568373     Document Type: Article

References (35)

1. Gurtner GC, Werner S, Barrandon Y, Longaker MT. Wound repair and regeneration. Nature. 2008 ; 453: 314-321
2. Larson BJ, Longaker MT, Lorenz HP. Scarless fetal wound healing: a basic science review. Plast Reconstr Surg. 2010 ; 126: 1172-1180
3. Mias C, Lairez O, Trouche E, et al. Mesenchymal stem cells promote matrix metalloproteinase secretion by cardiac fibroblasts and reduce cardiac ventricular fibrosis after myocardial infarction. Stem Cells. 2009 ; 27: 2734-2743
4. Gabbiani G. The myofibroblast in wound healing and fibrocontractive diseases. J Pathol. 2003 ; 200: 500-503
5. Brown RD, Ambler SK, Mitchell MD, Long CS. The cardiac fibroblast: therapeutic target in myocardial remodeling and failure. Annu Rev Pharmacol Toxicol. 2005 ; 45: 657-687
6. Al-Nbaheen M, Vishnubalaji R, Ali D. Human stromal (mesenchymal) stem cells from bone marrow, adipose tissue and skin exhibit differences in molecular phenotype and differentiation potential. Stem Cell Rev. 2013 ; 9: 32-43
7. Parekkadan B, Milwid JM. Mesenchymal stem cells as therapeutics. Annu Rev Biomed Eng. 2010 ; 12: 87-117
8. Baraniak PR, McDevitt TC. Stem cell paracrine actions and tissue regeneration. Regen Med. 2010 ; 5: 121-143
9. Timmers L, Lim SK, Arslan F, et al. Reduction of myocardial infarct size by human mesenchymal stem cell conditioned medium. Stem Cell Res. 2007 ; 1: 129-137
10. Li L, Zhang Y, Li Y, et al. Mesenchymal stem cell transplantation attenuates cardiac fibrosis associated with isoproterenol-induced global heart failure. Transpl Int. 2008 ; 21: 1181-1189
11. Ortiz LA, Gambelli F, McBride C, et al. Mesenchymal stem cell engraftment in lung is enhanced in response to bleomycin exposure and ameliorates its fibrotic effects. Proc Natl Acad Sci U S A. 2003 ; 100: 8407-8411
12. Kim SY, Lee JH, Kim HJ, et al. Mesenchymal stem cell-conditioned media recovers lung fibroblasts from cigarette smoke-induced damage. Am J Physiol Lung Cell Mol Physiol. 2012 ; 302: L891 - L908
13. Oyagi S, Hirose M, Kojima M, et al. Therapeutic effect of transplanting HGF-treated bone marrow mesenchymal cells into CCl4-injured rats. J Hepatol. 2006 ; 44: 742-748
14. Abdel Aziz MT, Atta HM, Mahfouz S, et al. Therapeutic potential of bone marrow-derived mesenchymal stem cells on experimental liver fibrosis. Clin Biochem. 2007 ; 40: 893-899
15. Ninichuk V, Gross O, Segerer S, et al. Multipotent mesenchymal stem cells reduce interstitial fibrosis but do not delay progression of chronic kidney disease in collagen4A3-deficient mice. Kidney Int. 2006 ; 70: 121-129
16. Semedo P, Correa-Costa M, Cenedeze MA, et al. Mesenchymal stem cells attenuate renal fibrosis through immune modulation and remodeling properties in a rat remnant kidney model. Stem Cells. 2009 ; 27: 3063-3073
17. Watson SL, Marcal H, Sarris M, Girolamo ND, M Coroneo TC, Wakefield D. The effect of mesenchymal stem cell conditioned media on corneal stromal fibroblast wound healing activities. Br J Ophthalmol. 2010 ; 94: 1067-1073
18. Shah M, Foreman DM, Ferguson MW. Neutralisation of TGF-beta 1 and TGF-beta 2 or exogenous addition of TGF-beta 3 to cutaneous rat wounds reduces scarring. J Cell Sci. 1995 ; 108: 985-1002
19. Takeda Y, Mori T, Imabayashi H, et al. Can the life span of human marrow stromal cells be prolonged by bmi-1, E6, E7, and/or telomerase without affecting cardiomyogenic differentiation?. J Gene Med. 2004 ; 6: 833-845
20. Bocelli-Tyndall C, Bracci L, Spagnoli G, et al. Bone marrow mesenchymal stromal cells (BM-MSCs) from healthy donors and auto-immune disease patients reduce the proliferation of autologous- and allogeneic-stimulated lymphocytes in vitro. Rheumatology. 2007 ; 46: 403-408
21. Crisostomo PR, Wang Y, Markel TA, Wang M, Lahm T, Meldrum DR. Human mesenchymal stem cells stimulated by TNF-α, LPS, or hypoxia produce growth factors by an NF κB- but not JNK-dependent mechanism. Am J Physiol Cell Physiol. 2008 ; 294: C675 - C682
22. Dienus K, Bayat A, Gilmore BF, Seifert O. Increased expression of fibroblast activation protein-alpha in keloid fibroblasts: implications for development of a novel treatment option. Arch Dermatol Res. 2010 ; 302: 725-731
23. Tang B, Zhu B, Liang Y, et al. Asiaticoside suppresses collagen expression and TGF-b/Smad signaling through inducing Smad7 and inhibiting TGF-bRI and TGF-bRII in keloid fibroblasts. Arch Dermatol Res. 2011 ; 303: 563-572
24. Wang S, Wu H, Liu Y, et al. Expression of USP2-69 in mesangial cells in vivo and in vitro. Pathol Int. 2010 ; 60: 184-192
25. Pittenger MF, Mackay AM, Beck SC, et al. Multilineage potential of adult human mesenchymal stem cells. Science. 1999 ; 284: 143-147
26. Colter DC, Class R, DiGirolamo CM, Prockop DJ. Rapid expansion of recycling stem cells in cultures of plastic-adherent cells from human bone marrow. Proc Natl Acad Sci U S A. 2000 ; 97: 3213-3218
27. Yamamoto T, Kuroda M, Nishioka K. Animal model of sclerotic skin. III: histopathological comparison of bleomycin-induced scleroderma in various mice strains. Arch Dermatol Res. 2000 ; 292: 535-541
28. Hsiao ST, Asgari A, Lokmic Z, et al. Comparative analysis of paracrine factor expression in human adult mesenchymal stem cells derived from bone marrow, adipose, and dermal tissue. Stem Cells Dev. 2012 ; 21: 2189-2203
29. Baraniak PR, McDevitt TC. Stem cell paracrine actions and tissue regeneration. Regen Med. 2010 ; 5: 121-143
30. Leclerc T, Thepenier C, Jault P, et al. Cell therapy of burns. Cell Prolif. 2011 ; 44: 48-54
31. Liu W, Wang DR, Cao YL. TGF-β: a fibrotic factor in wound scarring and a potential target for anti-scarring gene therapy. Curr Gene Ther. 2004 ; 4: 123-136
32. Seong J, Kim SH, Chung EJ, Lee WJ, Suh CO. Early alteration in TGF-β mRNA expression in irradiated rat liver. Int J Radiat Oncol. 2000 ; 46: 639-643
33. Rhett JM, Ghatnekar GS, Palatinus JA, O'Quinn M, Yost MJ, Gourdie RG. Novel therapies for scar reduction and regenerative healing of skin wounds. Cell. 2008 ; 26: 173-180
34. Ferguson MWJ, Duncan J, Bond J, et al. Prophylactic administration of avotermin for improvement of skin scarring: three double-blind, placebo-controlled, phase I/II studies. Lancet. 2009 ; 373: 1264-1274
35. Ludwicka A, Trojanowska M, Smith EA, et al. Growth and characterization of fibroblasts obtained from bronchoalveolar lavage of patients with scleroderma. J Rheumatol. 1992 ; 19: 1716-1723