Bimolecular fluorescence complementation (BiFC) in live Drosophila embryos

Methods in Molecular Biology

Volumn 1196, Issue , 2014, Pages 307-318

  Duffraisse, Marilyne ^a   Hudry, Bruno ^a   Merabet, Samir ^a  

^a NONE

Author keywords

BiFC; Drosophila; EMSA; Hox; Transcription

Indexed keywords

DROSOPHILA PROTEIN; GREEN FLUORESCENT PROTEIN; HOX PROTEIN; PBC PROTEIN; UNCLASSIFIED DRUG; VENUS FLUORESCENT PROTEIN; HOMEODOMAIN PROTEIN;

ARTICLE; BIMOLECULAR FLUORESCENCE COMPLEMENTATION; CLONING VECTOR; CONTROLLED STUDY; DROSOPHILA; EMBRYO; FLUORESCENCE IMAGING; GEL MOBILITY SHIFT ASSAY; IMMUNOHISTOCHEMISTRY; MOLECULAR IMAGING; NONHUMAN; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; PROTEIN SYNTHESIS; ANIMAL; ANIMAL EMBRYO; GENE EXPRESSION; GENETIC TRANSCRIPTION; GENETICS; METABOLISM; PROCEDURES; PROTEIN ANALYSIS;

DROSOPHILA MELANOGASTER;

ANIMALS; DROSOPHILA; ELECTROPHORETIC MOBILITY SHIFT ASSAY; EMBRYO, NONMAMMALIAN; GENE EXPRESSION; HOMEODOMAIN PROTEINS; PROTEIN INTERACTION MAPPING; TRANSCRIPTION, GENETIC;

EID: 84927143040     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-1242-1_19     Document Type: Article

References (13)

1. Kerppola TK (2008) Bimolecular fluorescence complementation (BiFC) analysis as a probe of protein interactions in living cells. Annu Rev Biophys 37:465–487
2. Kodama Y, Hu CD (2012) Bimolecular fluorescence complementation (BiFC): a 5-year update and future perspectives. Biotechniques 53:285–298
3. Hu CD, Chinenov Y, Kerppola TK (2002) Visualization of interactions among bZIP and Rel family proteins in living cells using bimolecular fluorescence complementation. Mol Cell 9:789–798
4. Shaner NC, Lambert GG, Chammas A, Ni Y, Cranfill PJ et al (2013) A bright monomeric green fluorescent protein derived from Branchiostoma lanceolatum. Nat Methods 10:407–409
5. Zhou J, Lin J, Zhou C, Deng X, Xia B (2011) An improved bimolecular fluorescence complementation tool based on superfolder green fluorescent protein. Acta Biochim Biophys Sin (Shanghai) 43:239–244
6. Kim J, Zhao T, Petralia RS, Yu Y, Peng H et al (2012) mGRASP enables mapping mammalian synaptic connectivity with light microscopy. Nat Methods 9:96–102
7. Shyu YJ, Liu H, Deng X, Hu CD (2006) Identification of new fluorescent protein fragments for bimolecular fluorescence complementation analysis under physiological conditions. Biotechniques 40:61–66
8. Hudry B, Viala S, Graba Y, Merabet S (2011) Visualization of protein interactions in living Drosophila embryos by the bimolecular fluorescence complementation assay. BMC Biol 9:5
9. Bischof J, Maeda RK, Hediger M, Karch F, Basler K (2007) An optimized transgenesis system for Drosophila using germ-line-specific phiC31 integrases. Proc Natl Acad Sci U S A 104:3312–3317
10. Bischof J, Bjorklund M, Furger E, Schertel C, Taipale J et al (2013) A versatile platform for creating a comprehensive UAS-ORFeome library in Drosophila. Development 140: 2434–2442
11. Gohl C, Banovic D, Grevelhorster A, Bogdan S (2010) WAVE forms hetero- and homooligomeric complexes at integrin junctions in Drosophila visualized by bimolecular fluorescence complementation. J Biol Chem 285: 40171–40179
12. de Navas L, Foronda D, Suzanne M, Sanchez- Herrero E (2006) A simple and efficient method to identify replacements of P-lacZ by P-Gal4 lines allows obtaining Gal4 insertions in the bithorax complex of Drosophila. Mech Dev 123:860–867
13. Gebelein B, Culi J, Ryoo HD, Zhang W, Mann RS (2002) Specificity of Distalless repression and limb primordia development by abdominal Hox proteins. Dev Cell 3: 487–498