Generation of living cell arrays for atomic force microscopy studies

Nature Protocols

Volumn 10, Issue 1, 2015, Pages 199-204

  Formosa, Cécile ^a,b,c,d   Pillet, Flavien ^b,e   Schiavone, Marion ^a,b,f   Duval, Raphaël E ^c,d,g   Ressier, Laurence ^b,h   Dague, Etienne ^a,b  

^a LAAS CNRS   (France)

^b UNIVERSITÉ DE TOULOUSE   (France)

^c NANCY UNIVERSITÉ   (France)

^d UNIVERSITÉ DE LORRAINE   (France)

^e INSTITUT DE PHARMACOLOGIE ET DE BIOLOGIE STRUCTURALE   (France)

^f CNRS   (France)

^g ABC PlatformH   (France)

^h Institut National des Sciences Appliquées   (France)

Author keywords

[No Author keywords available]

Indexed keywords

DIMETICONE; SILICON; BAYSILON;

ARTICLE; ATOMIC FORCE MICROSCOPY; BACTERIAL SPORE; CANDIDA ALBICANS; IMMOBILIZED CELL; MORPHOLOGY; NONHUMAN; PRIORITY JOURNAL; YEAST CELL; METABOLISM; PROCEDURES; TISSUE MICROARRAY;

BACTERIA (MICROORGANISMS);

CELLS, IMMOBILIZED; DIMETHYLPOLYSILOXANES; MICROSCOPY, ATOMIC FORCE; TISSUE ARRAY ANALYSIS;

EID: 84925954410     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2015.004     Document Type: Article

References (27)

1. Binnig, G., Quate, C.F., Gerber, C. Atomic force microscope. Phys. Rev. Lett. 56, 930-934 (1986).
2. Gerber, C., Lang, H.P. How the doors to the nanoworld were opened. Nat. Nanotechnol. 1, 3-5 (2006).
3. Louise Meyer, R. et al. Immobilisation of living bacteria for AFM imaging under physiological conditions. Ultramicroscopy 110, 1349-1357 (2010).
4. Doktycz, M.J. et al. AFM imaging of bacteria in liquid media immobilized on gelatin coated mica surfaces. Ultramicroscopy 97, 209-216 (2003).
5. Kasas, S., Ikai, A. A method for anchoring round shaped cells for atomic force microscope imaging. Biophys. J. 68, 1678-1680 (1995).
6. Kailas, L. et al. Immobilizing live bacteria for AFM imaging of cellular processes. Ultramicroscopy 109, 775-780 (2009).
7. Francius, G., Domenech, O., Mingeot-Leclercq, M.P., Dufrêne, Y.F. Direct observation of Staphylococcus aureus cell wall digestion by lysostaphin. J. Bacteriol. 190, 7904-7909 (2008).
8. Alsteens, D. et al. Structure, cell wall elasticity and polysaccharide properties of living yeasts cells, as probed by AFM. Nanotechnology 19, 384005 (2008).
9. Dague, E., Alsteens, D., Latgé, J.-P., Dufrêne, Y.F. High-resolution cell surface dynamics of germinating Aspergillus fumigatus conidia. Biophys. J. 94, 656-660 (2008).
10. Gilbert, Y. et al. Single-molecule force spectroscopy and imaging of the vancomycin/d-Ala-d-Ala interaction. Nano Lett. 7, 796-801 (2007).
11. Dague, E. et al. Assembly of live micro-organisms on microstructured PDMS stamps by convective/capillary deposition for AFM bio-experiments. Nanotechnology 22, 395102 (2011).
12. Francois, J.M. et al. Use of atomic force microscopy (AFM) to explore cell wall properties and response to stress in the yeast Saccharomyces cerevisiae. Curr. Genet. 59, 187-196 (2013).
13. Pillet, F., Chopinet, L., Formosa, C., Dague, É. Atomic force microscopy and pharmacology: from microbiology to cancerology. Biochim. Biophys. Acta 1840, 1028-1050 (2014).
14. Chopinet, L., Formosa, C., Rols, M.P., Duval, R.E., Dague, E. Imaging living cells surface and quantifying its properties at high resolution using AFM in QITM mode. Micron 48, 26-33 (2013).
15. Formosa, C. et al. Nanoscale effects of Caspofungin against two yeast species, Saccharomyces cerevisiae and Candida albicans. Antimicrob. Agents Chemother. 57, 3498-3506 (2013).
16. Pillet, F. et al. Uncovering by atomic force microscopy of an original circular structure at the yeast cell surface in response to heat shock. BMC Biol. 12, 6 (2014).
17. Beauvais, A. et al. Deletion of the ?-(1, 3)-glucan synthase genes induces a restructuring of the conidial cell wall responsible for the avirulence of Aspergillus fumigatus. PLoS Pathog. 9, e1003716 (2013).
18. Dufrêne, Y.F., Martínez-Martín, D., Medalsy, I., Alsteens, D., Müller, D.J. Multiparametric imaging of biological systems by force-distance curve-based AFM. Nat. Methods 10, 847-854 (2013).
19. Dufrêne, Y.F. Atomic force microscopy and chemical force microscopy of microbial cells. Nat. Protoc. 3, 1132-1138 (2008).
20. Francius, G. et al. Stretching polysaccharides on live cells using single-molecule force spectroscopy. Nat. Protoc. 4, 939-946 (2009).
21. Beaussart, A. et al. Quantifying the forces guiding microbial cell adhesion using single-cell force spectroscopy. Nat. Protoc. 9, 1049-1055 (2014).
22. Roduit, C. et al. OpenFovea: open-source AFM data processing software. Nat. Methods 9, 774-775 (2012).
23. Cai, D.K., Neyer, A., Kuckuk, R., Heise, H.M. Optical absorption in transparent PDMS materials applied for multimode waveguides fabrication. Opt. Mater. 30, 1157-1161 (2008).
24. Chabinyc, M.L. et al. An integrated fluorescence detection system in poly(dimethylsiloxane) for microfluidic applications. Anal. Chem. 73, 4491-4498 (2001).
25. Liu, S., Wang, Y. Application of AFM in microbiology: a review. Scanning 32, 61-73 (2010).
26. JPK Instruments. QITM mode-quantitative imaging with the Nanowizard 3 AFM. http://usa.jpk.com/index.download.419baba450fa6c06a245970866047614
27. Formosa, C. et al. Multiparametric imaging of adhesive nanodomains at the surface of Candida albicans by atomic force microscopy. Nanomed. Nanotechnol. Biol. Med. doi:10.1016/j.nano.2014.07.008 (4 August 2014).