Quantifying the forces guiding microbial cell adhesion using single-cell force spectroscopy

Nature Protocols

Volumn 9, Issue 5, 2014, Pages 1049-1055

  Beaussart, Audrey ^a   El Kirat Chatel, Sofiane ^a   Sullan, Ruby May A ^a   Alsteens, David ^a   Herman, Philippe ^a   Derclaye, Sylvie ^a   Dufrêne, Yves F ^a  

^a UNIVERSITÉ CATHOLIQUE DE LOUVAIN   (Belgium)

Author keywords

[No Author keywords available]

Indexed keywords

POLYDOPAMINE; POLYMER; UNCLASSIFIED DRUG;

ARTICLE; ATOMIC FORCE MICROSCOPY; BACTERIAL CELL; CANDIDA ALBICANS; CELL ADHESION FORCE; CELL INTERACTION; CELL PROBE; IMMOBILIZED CELL; LACTOBACILLUS PLANTARUM; MICROBIAL ADHESION; MOLECULAR IMAGING; MOLECULAR PROBE; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; SINGLE CELL ANALYSIS; SINGLE CELL FORCE SPECTROSCOPY; SPECTROSCOPY; STAPHYLOCOCCUS EPIDERMIDIS; STATIC ELECTRICITY; BIOMECHANICS; CELL ADHESION; PHYSIOLOGY; PROCEDURES; ULTRASTRUCTURE;

BIOMECHANICAL PHENOMENA; CANDIDA ALBICANS; CELL ADHESION; LACTOBACILLUS PLANTARUM; MICROSCOPY, ATOMIC FORCE; SINGLE-CELL ANALYSIS; STAPHYLOCOCCUS EPIDERMIDIS;

EID: 84899525206     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2014.066     Document Type: Article

References (31)

1. Busscher, H.J., Norde, W. & van der Mei, H.C. Specific molecular recognition and nonspecific contributions to bacterial interaction forces. Appl. Environ. Microb. 74, 2559-2564 (2008).
2. Busscher, H.J & van der Mei, H.C. How do bacteria know they are on a surface and regulate their response to an adhering state? PLoS Pathog. 8, e1002440 (2012).
3. Camesano, T.A., Liu, Y. & Datta, M. Measuring bacterial adhesion at environmental interfaces with single-cell and single-molecule techniques. Adv. Water. Resour. 30, 1470-1491 (2007).
4. Brehm-Stecher, B.F. & Johnson, E.A. Single-cell microbiology: Tools, technologies, and applications. Microbiol. Mol. Biol. R. 68, 538-559 (2004).
5. Lidstrom, M.E. & Konopka, M.C. The role of physiological heterogeneity in microbial population behavior. Nat. Chem. Biol. 6, 705-712 (2010).
6. Müller, D.J. & Dufrêne, Y.F. Atomic force microscopy as a multifunctional molecular toolbox in nanobiotechnology. Nat. Nanotechnol. 3, 261-269 (2008).
7. Müller, D.J., Helenius, J., Alsteens, D. & Dufrêne, Y.F. Force probing surfaces of living cells to molecular resolution. Nat. Chem. Biol. 5, 383-390 (2009).
8. Benoit, M., Gabriel, D., Gerisch, G. & Gaub, H.E. Discrete interactions in cell adhesion measured by single-molecule force spectroscopy. Nat. Cell Biol. 2, 313-317 (2000).
9. Benoit, M. & Gaub, H.E. Measuring cell adhesion forces with the atomic force microscope at the molecular level. Cells Tissues Organs 172, 174-189 (2002).
10. Helenius, J., Heisenberg, C.P., Gaub, H.E. & Muller, D.J. Single-cell force spectroscopy. J. Cell. Sci. 121, 1785-1791 (2008).
11. Le, D.T.L., Guérardel, Y., Loubière, P., Mercier-Bonin, M. & Dague, E. Measuring kinetic dissociation/association constants between Lactococcus lactis bacteria and mucins using living cell probes. Biophys. J. 101, 2843-2853 (2011).
12. Ovchinnikova, E.S., Krom, B.P., van der Mei, H.C. & Busscher, H.J. Force microscopic and thermodynamic analysis of the adhesion between Pseudomonas aeruginosa and Candida albicans. Soft Matter 8, 6454-6461 (2012).
13. Lower, S.K., Hochella, M.F. Jr & Beveridge, T.J. Bacterial recognition of mineral surfaces: Nanoscale interactions between Shewanella and α-FeOOH. Science 292, 1360-1363 (2001).
14. Emerson, R.J. IV et al. Microscale correlation between surface chemistry, texture, and the adhesive strength of Staphylococcus epidermidis. Langmuir 22, 11311-11321 (2006).
15. Bowen, W.R., Lovitt, R.W. & Wright, C.J. Atomic force microscopy study of the adhesion of Saccharomyces cerevisiae. J. Colloid Inter. Sci. 237, 54-61 (2001).
16. Razatos, A., Ong, Y.L., Sharma, M.M. & Georgiou, G. Molecular determinants of bacterial adhesion monitored by atomic force microscopy. Proc. Natl. Acad. Sci. USA 95, 11059-11064 (1998).
17. Kang, S. & Elimelech, M. Bioinspired single bacterial cell force spectroscopy. Langmuir 25, 9656-9659 (2009).
18. Beaussart, A. et al. Single-cell force spectroscopy of probiotic bacteria. Biophys. J. 104, 1886-1892 (2013).
19. Ong, Y.L., Razatos, A., Georgiou, G. & Sharma, M.M. Adhesion forces between E. coli bacteria and biomaterial surfaces. Langmuir 15, 2719-2725 (1999).
20. Meister, A. et al. FluidFM: Combining atomic force microscopy and nanofluidics in a universal liquid delivery system for single cell applications and beyond. Nano Lett. 9, 2501-2507 (2009).
21. Dörig, P. et al. Force-controlled spatial manipulation of viable mammalian cells and micro-organisms by means of FluidFM technology. Appl. Phys. Lett. 97, 023701 (2010).
22. Beaussart, A. et al. Single-cell force spectroscopy of the medically important Staphylococcus epidermidis-Candida albicans interaction. Nanoscale 5, 10894-10900 (2013).
23. Herman, P. et al. Forces driving the attachment of Staphylococcus epidermidis to fibrinogen-coated surfaces. Langmuir 29, 13018-13022 (2013).
24. Alsteens, D., van Dijck, P., Lipke, P.N. & Dufrêne, Y.F. Quantifying the forces driving cell-cell adhesion in a fungal pathogen. Langmuir 29, 13473-13480 (2013).
25. Alsteens, D. et al. Single-cell force spectroscopy of Als-mediated fungal adhesion. Anal. Methods 5, 3657-3662 (2013).
26. Lee, H., Dellatore, S.M., Miller, W.M. & Messersmith, P.B. Mussel-inspired surface chemistry for multifunctional coatings. Science 318, 426-430 (2007).
27. Francius, G. et al. Stretching polysaccharides on live cells using single molecule force spectroscopy. Nat. Protoc. 4, 939-946 (2009).
28. Rief, M., Oesterhelt, F., Heymann, B. & Gaub, H.E. Single molecule force spectroscopy on polysaccharides by atomic force microscopy. Science 275, 1295-1297 (1997).
29. Marszalek, P.E., Oberhauser, A.F., Pang, Y.P. & Fernandez, J.M. Polysaccharide elasticity governed by chair-boat transitions of the glucopyranose ring. Nature 396, 661-664 (1998).
30. Rief, M., Gautel, M., Oesterhelt, F., Fernandez, J.M. & Gaub, H.E. Reversible unfolding of individual titin immunoglobulin domains by AFM. Science 276, 1109-1112 (1997).
31. Oberhauser, A.F., Hansma, P.K., Carrion-Vazquez, M. & Fernandez, J.M. Stepwise unfolding of titin under force-clamp atomic force microscopy. Proc. Natl. Acad. Sci. USA 98, 468-472 (2001).