SILAC labeling of yeast for the study of membrane protein complexes

Methods in Molecular Biology

Volumn 1188, Issue , 2014, Pages 23-46

  Oeljeklaus, Silke ^a   Schummer, Andreas ^a   Suppanz, Ida ^a   Warscheid, Bettina ^a  

^a UNIVERSITY OF FREIBURG   (Germany)

Author keywords

Affinity purification mass spectrometry; Epitope tagging; Gene disruption; Membrane protein complex; Quantitative proteomics; SILAC; Yeast

Indexed keywords

AMINO ACID; EPITOPE; MEMBRANE PROTEIN; STABLE ISOTOPE; SACCHAROMYCES CEREVISIAE PROTEIN; TRYPSIN;

ARTICLE; CELL CULTURE; CYTOLOGY; FUNGAL STRAIN; ISOTOPE LABELING; MASS SPECTROMETRY; MOLECULAR DYNAMICS; MOLECULAR MECHANICS; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN INTERACTION; PROTEIN PURIFICATION; PROTEOMICS; SACCHAROMYCES CEREVISIAE; STABLE ISOTOPE LABELING BY AMINO ACID IN CELL CULTURE; ANALYSIS; CHEMISTRY; CULTURE TECHNIQUE; GENETICS; GENOMICS; LIQUID CHROMATOGRAPHY; METABOLISM; PROCEDURES; PROTEIN DEGRADATION; TANDEM MASS SPECTROMETRY;

AMINO ACIDS; ANALYTIC SAMPLE PREPARATION METHODS; CELLS, CULTURED; CHROMATOGRAPHY, LIQUID; CULTURE TECHNIQUES; GENOMICS; ISOTOPE LABELING; MEMBRANE PROTEINS; PROTEOLYSIS; PROTEOMICS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; TANDEM MASS SPECTROMETRY; TRYPSIN;

EID: 84925884050     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-1142-4_3     Document Type: Article

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