메뉴 건너뛰기




Volumn 1179, Issue , 2014, Pages 139-149

OmniChange: Simultaneous site saturation of up to five codons

Author keywords

Directed evolution; Focused mutagenesis; Ligase independent assembly; Multi site saturation mutagenesis; Sequence independent hybridization

Indexed keywords

ALCOHOL; DNA; DNA FRAGMENT; DOUBLE STRANDED DNA; ENDONUCLEASE; IODINE; LIGASE; NUCLEOTIDE; OLIGONUCLEOTIDE; PHOSPHOROTHIOIC ACID; CODON;

EID: 84925883585     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-1053-3_9     Document Type: Article
Times cited : (8)

References (7)
  • 1
    • 33845288649 scopus 로고    scopus 로고
    • Iterative saturation mutagenesis on the basis of B factors as a strategy for increasing protein thermostability
    • Reetz MT, Carballeira JD, Vogel A (2006) Iterative saturation mutagenesis on the basis of B factors as a strategy for increasing protein thermostability. Angew Chem Int Ed Engl 45: 7745-7751
    • (2006) Angew Chem Int Ed Engl , vol.45 , pp. 7745-7751
    • Reetz, M.T.1    Carballeira, J.D.2    Vogel, A.3
  • 2
    • 65249152992 scopus 로고    scopus 로고
    • Advances in generating functional diversity for directed protein evolution
    • Shivange AV, Marienhagen J, Mundhada H et al (2009) Advances in generating functional diversity for directed protein evolution. Curr Opin Chem Biol 13:19-25
    • (2009) Curr Opin Chem Biol , vol.13 , pp. 19-25
    • Shivange, A.V.1    Marienhagen, J.2    Mundhada, H.3
  • 3
    • 80054785847 scopus 로고    scopus 로고
    • OmniChange: The sequence independent method for simultaneous site-saturation of five codons
    • Dennig A, Shivange AV, Marienhagen J, Schwaneberg U (2011) OmniChange: the sequence independent method for simultaneous site-saturation of five codons. PLoS One 6:e26222
    • (2011) PLoS One , vol.6
    • Dennig, A.1    Shivange, A.V.2    Marienhagen, J.3    Schwaneberg, U.4
  • 4
    • 22144485602 scopus 로고    scopus 로고
    • Expanding the range of substrate acceptance of enzymes: Combinatorial active-site saturation test
    • Reetz MT, Bocola M, Carballeira JD et al (2005) Expanding the range of substrate acceptance of enzymes: combinatorial active-site saturation test. Angew Chem Int Ed Engl 44:4192-4196
    • (2005) Angew Chem Int Ed Engl , vol.44 , pp. 4192-4196
    • Reetz, M.T.1    Bocola, M.2    Carballeira, J.D.3
  • 5
    • 0036841628 scopus 로고    scopus 로고
    • Creating randomized amino acid libraries with the quikchange multi site- directed mutagenesis kit
    • Hogrefe HH, Cline J, Youngblood GL, Allen RM (2002) Creating randomized amino acid libraries with the QuikChange Multi Site- Directed Mutagenesis Kit. Biotechniques 33: 1158-1160
    • (2002) Biotechniques , vol.33 , pp. 1158-1160
    • Hogrefe, H.H.1    Cline, J.2    Youngblood, G.L.3    Allen, R.M.4
  • 6
    • 0024508604 scopus 로고
    • Phosphorothioates in molecular biology
    • Eckstein F, Gish G (1989) Phosphorothioates in molecular biology. Trends Biochem Sci 14:97-100
    • (1989) Trends Biochem Sci , vol.14 , pp. 97-100
    • Eckstein, F.1    Gish, G.2
  • 7
    • 84855952259 scopus 로고    scopus 로고
    • P450(BM3) (CYP102A1): Connecting the dots
    • Whitehouse CJ, Bell SG, Wong LL (2012) P450(BM3) (CYP102A1): connecting the dots. Chem Soc Rev 41:1218-1260
    • (2012) Chem Soc Rev , vol.41 , pp. 1218-1260
    • Whitehouse, C.J.1    Bell, S.G.2    Wong, L.L.3


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.