Extreme PCR: A breakthrough innovation for outbreaks?

Clinical Chemistry

Volumn 61, Issue 4, 2015, Pages 674-676

  Kumar, Vikram Sheel ^a   Webster, Molly ^b  

^a BOSTON CHILDREN S HOSPITAL   (United States)

^b Science writer and producer   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; CHEMISTRY; GEL ELECTROPHORESIS; HUMAN; MEDICAL TECHNOLOGY; MICROFLUIDICS; POLYMERASE CHAIN REACTION; POLYMERASE CHAIN REACTION SYSTEM; DEVICES; EBOLAVIRUS; EPIDEMIC; GENETICS; PROCEDURES;

VIRUS DNA;

DISEASE OUTBREAKS; DNA, VIRAL; EBOLAVIRUS; HUMANS; MICROFLUIDICS; POLYMERASE CHAIN REACTION;

EID: 84925705105     PISSN: 00099147     EISSN: 15308561     Source Type: Journal    
DOI: 10.1373/clinchem.2014.236950     Document Type: Article

References (7)

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2. Mullis KB, Ferré F, Gibbs RA, editors. The polymerase chain reaction. Boston: Birkhäuser; 1994.
3. Wittwer CT, Fillmore GC, Garling DJ. Minimizing the time required for DNA amplification by efficient heat transfer to small samples. Anal Biochem 1990; 186:328-31.
4. Wittwer CT, Garling DJ. Rapid cycle DNA amplification: time and temperature optimization. Biotechniques 1991; 10:76-83.
5. Farrar JS, Wittwer CT. Extreme PCR: efficient and specific DNA amplification in 15-60 seconds. Clin Chem 2015; 61:145-53.
6. JF Mackay. Taking it to the extreme: PCR at wittwerspeed. Clin Chem 2015; 61:4-5.
7. Sundberg SO, Wittwer CT, Howell R.M., Huuskonen J., Pryor RJ, Farrar JS, et al. Microfluidic genotypingby rapid serial PCR and high-speed melting analysis. Clin Chem 2014; 60:1306-13.