Taking it to the extreme: PCR at wittwerspeed

Clinical Chemistry

Volumn 61, Issue 1, 2015, Pages 4-5

  Mackay, John F ^a  

^a Dnature Diagnostics and Research   (New Zealand)

Author keywords

[No Author keywords available]

Indexed keywords

CHEMICAL MODIFICATION; DNA DENATURATION; EDITORIAL; FINGER DERMATOGLYPHICS; GEL ELECTROPHORESIS; HIGH RESOLUTION MELTING ANALYSIS; HUMAN; KINETICS; POLYMERASE CHAIN REACTION; POLYMERASE CHAIN REACTION SYSTEM; REAL TIME POLYMERASE CHAIN REACTION; SEXUAL HEALTH; PROCEDURES; STANDARDS; TIME; TRENDS;

REAL-TIME POLYMERASE CHAIN REACTION; TIME FACTORS;

EID: 84920545817     PISSN: 00099147     EISSN: 15308561     Source Type: Journal    
DOI: 10.1373/clinchem.2014.233338     Document Type: Editorial

References (14)

1. Wittwer CT, Garling DJ. Rapid cycle DNA amplification: time and temperature optimization. Biotechniques 1991;10:76-83.
2. Lay MJ, Wittwer CT. Real-time fluorescence genotyping of factor V Leiden during rapidcycle PCR. Clin Chem 1997;43:2262-7.
3. Wittwer CT, Reed GH, Gundry CN, Vandersteen JG, Pryor RJ. High-resolution genotyping by amplicon melting analysis using LCGreen. Clin Chem 2003;49:853-60.
4. Zhou L, Errigo RJ, Lu H, Poritz MA, Seipp MT, Wittwer CT. Snapback primer genotyping with saturating DNA dye and melting analysis. Clin Chem 2008;54:1648-56.
5. Landau M. Inspiring minds. Clin Chem 2009;55:1744-6.
6. Neuzil P, Zhang C, Pipper J, Oh S, Zhuo L. Ultra fast miniaturized real-time PCR: 40 cycles in less than six minutes. Nucl Acids Res 2006;34:e77.
7. Wheeler EK, Hara CA, Frank J, Deotte J, Hall SB, Benett W, Spadaccini C, Beer NR. Under-three minute PCR: probing the limits of fast amplification. Analyst 2011;136: 3707-12.
8. Stehr J, Hrelescu C, Sperling RA, Raschke G, Wunderlich M, Nichtl A, et al. Gold nanostoves for microsecond DNA melting analysis. Nano Lett 2008;8:619-23.
9. Montgomery JL, Wittwer CT. Influence of PCR reagents on DNA polymerase extension rates measured on real-time PCR instruments. Clin Chem 2014;60:334-40.
10. Farrar JS, Wittwer CT. Extreme PCR: efficient and specific DNA amplification in 15-60 seconds. Clin Chem 2015;61:145-53.
11. Dang C, Jayasena SD. Oligonucleotide inhibitors of Taq DNA polymerase facilitate detection of low copy number targets by PCR. J Mol. Biol 1996;264:268-78.
12. Huhmer AFR, Landers JP. Noncontact infrared-mediated thermocycling for effective polymerase chain reaction amplification of DNA in nanoliter volumes. Anal Chem 2000;72:5507-12.
13. Moss D, Harbison SA, Saul DJ. An easily automated, closed-tube forensic DNA extraction procedure using a thermostable proteinase. Int J Legal Med 2003;117:340-9.
14. Chomczynski P, Rymaszewski M. Alkaline polyethylene glycol-based method for direct PCR from bacteria, eukaryotic tissue samples, and whole blood. Bio Techniques 2006;40:454-8.