Preventing T7 RNA polymerase read-through transcription-A synthetic termination signal capable of improving bioprocess stability

ACS Synthetic Biology

Volumn 4, Issue 3, 2015, Pages 265-273

  Mairhofer, Juergen ^a,b   Wittwer, Alexander ^a,b   Cserjan Puschmann, Monika ^a,b   Striedner, Gerald ^a,b  

^a UNIVERSITY OF NATURAL RESOURCES AND LIFE SCIENCES   (Austria)

^b AUSTRIAN CENTRE OF INDUSTRIAL BIOTECHNOLOGY   (Austria)

Author keywords

contextuality; insulator; plasmid vector design; read through transcription; recombinant protein expression; T7 RNA polymerase; T7 terminator

Indexed keywords

PROTEIN; RNA POLYMERASE; BACTERIOPHAGE T7 RNA POLYMERASE; DNA DIRECTED RNA POLYMERASE; MESSENGER RNA; RECOMBINANT PROTEIN; VIRUS PROTEIN;

ARTICLE; BIOPROCESS; CONTROLLED STUDY; ESCHERICHIA COLI; FERMENTATION; GENE DOSAGE; GENE EXPRESSION; IN VITRO STUDY; MOLECULAR STABILITY; NONHUMAN; PRIORITY JOURNAL; RNA TRANSCRIPTION; TRANSCRIPTION TERMINATION; BIOLOGICAL MODEL; BIOTECHNOLOGY; GENETIC ENGINEERING; GENETIC TRANSCRIPTION; GENETICS; METABOLISM; PLASMID; REGULATOR GENE; SYNTHETIC BIOLOGY;

BIOTECHNOLOGY; DNA-DIRECTED RNA POLYMERASES; ESCHERICHIA COLI; GENETIC ENGINEERING; MODELS, GENETIC; PLASMIDS; RECOMBINANT PROTEINS; RNA, MESSENGER; SYNTHETIC BIOLOGY; TERMINATOR REGIONS, GENETIC; TRANSCRIPTION, GENETIC; VIRAL PROTEINS;

EID: 84925591812     PISSN: None     EISSN: 21615063     Source Type: Journal    
DOI: 10.1021/sb5000115     Document Type: Article

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