Purification of recombinant proteins with a multifunctional GFP tag

Methods in Molecular Biology

Volumn 1177, Issue , 2014, Pages 151-161

  Murayama, Takashi ^a   Kobayashi, Takuya ^a  

^a NONE

Author keywords

Affinity tag; Green fluorescent protein (GFP); Immobilized metal affinity chromatography (IMAC); Multifunctional GFP (mfGFP); Protein complex; Protein purification; Streptavidin binding peptide (SBP)

Indexed keywords

GREEN FLUORESCENT PROTEIN; RECOMBINANT PROTEIN; CARRIER PROTEIN; ENHANCED GREEN FLUORESCENT PROTEIN; HISTIDINE; STREPTAVIDIN-BINDING PEPTIDE;

AMINO ACID SEQUENCE; ARTICLE; CELL SUSPENSION; COLUMN CHROMATOGRAPHY; CONTROLLED STUDY; CRYOPRESERVATION; EUKARYOTIC CELL; FLUORESCENCE MICROSCOPY; GENE EXPRESSION SYSTEM; HEK293 CELL LINE; HUMAN; HUMAN CELL; PARTICULATE MATTER; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN PURIFICATION; STEREOSPECIFICITY; ULTRACENTRIFUGATION; AFFINITY CHROMATOGRAPHY; ANIMAL; BIOSYNTHESIS; CHEMISTRY; ESCHERICHIA COLI; GENETICS; ISOLATION AND PURIFICATION; MOLECULAR BIOLOGY; PROCEDURES;

EUKARYOTA; MAMMALIA; PROKARYOTA;

ANIMALS; CARRIER PROTEINS; CHROMATOGRAPHY, AFFINITY; ESCHERICHIA COLI; GREEN FLUORESCENT PROTEINS; HEK293 CELLS; HISTIDINE; HUMANS; MOLECULAR BIOLOGY; RECOMBINANT PROTEINS;

EID: 84925547795     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-1034-2_12     Document Type: Article

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