Potential of sequential treatment with minocycline and S. aureus specific phage lysin in eradication of MRSA biofilms: an in vitro study

Applied Microbiology and Biotechnology

Volumn 99, Issue 7, 2015, Pages 3201-3210

  Chopra, Shivani ^a   Harjai, Kusum ^a   Chhibber, Sanjay ^a  

^a PANJAB UNIVERSITY   (India)

Author keywords

Biofilm; Endolysin; Minocycline; MRSA

Indexed keywords

BACTERIA; CELL ADHESION; CELLS; CYTOLOGY;

ENDOLYSIN; INTERCELLULAR ADHESION; METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS; MINOCYCLINE; MRSA; SCANNING ELECTRON MICROSCOPIC; SEQUENTIAL TREATMENTS; WHEAT GERM AGGLUTININ;

BIOFILMS;

BACTERIAL DNA; ENDOLYSIN MR 10; HYDROLASE; MINOCYCLINE; UNCLASSIFIED DRUG; VIRUS ENZYME; ADHESIN; ANTIINFECTIVE AGENT; ENDOLYSIN; PROTEINASE;

ARTICLE; BACTERIAL CLEARANCE; BACTERIAL COUNT; BIOFILM; CONCENTRATION RESPONSE; CONTROLLED STUDY; EXTRACELLULAR MATRIX; IN VITRO STUDY; METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; NONHUMAN; TREATMENT DURATION; BACTERIOPHAGE; CHEMISTRY; DRUG EFFECTS; METABOLISM; PHYSIOLOGY;

ADHESINS, BACTERIAL; ANTI-BACTERIAL AGENTS; BACTERIOPHAGES; BIOFILMS; ENDOPEPTIDASES; METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS; MINOCYCLINE;

EID: 84925494003     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-015-6460-1     Document Type: Article

References (48)

1. Adams MH, Park BH (1956) An enzyme produced by a phage -host cell system. II. the properties of polysachharide depolymerase. Virol 2:719–736
2. Archer NK, Mazaitis MJ, Costerton WJ, Leid JG, Powers ME, Shirtliff ME (2011) Staphylococcus aureus biofilms: properties, regulation and roles in human disease. Virulence 2(5):445–459
3. Arciola CR, Campoccia D, Speziale P, Costerton JW, Montanaro L (2012) Biofilm formation in Staphylococcus implants infections. a review of molecular mechanisms and implications for biofilm-resistant materials. Biomaterials 33:5967–5982
4. Bedi MS, Verma V, Chhibber S (2009) Amoxicillin and specific bacteriophage can be used together for eradication of biofilm of Klebsiella pneumoniae B5055. World J Microbiol Biotechnol 25:1145–1151
5. Beenken KE, Dunman PM, McAleese F, Macapagal D, Murphy E, Projan SJ, Blevins JS, Smeltzer MS (2004) Global gene expression in Staphylococcus aureus biofilms. J Bacteriol 186:4665–4684
6. Brady RA, Leid JG, Kofonow J, Costerton JW, Shirtliff ME (2007) Immunoglobulins to surface-associated biofilm immunogens provide a novel means of visualization of methicillin-resistant Staphylococcus aureus biofilms. Appl Environ Microbiol 73:6612–6619
7. Cerca N (2005) Comparative assessment of antibiotic susceptibility of coagulase negative staphylococci in biofilm versus planktonic culture as assessed by bacterial enumeration nor rapid XTT colorimetry. J Antimicrob Chemother 56:331–336
8. Ceri H, Olson M, Morck D, Storey D, Read R, Buret A, Olson B (2001) The MBEC assay system: multiple equivalent biofilms for antibiotic and biocide susceptibility testing. Methods Enzymol 337:377–385
9. Chhibber S, Kaur T, Kaur S (2013) Co-therapy using lytic bacteriophage and linezolid: effective treatment in eliminating methicillin resistant Staphylococcus aureus (MRSA) from diabetic foot infections. PLoS ONE 8(2):e56022
10. Chopra S, Harjai K, Chhibber S (2014) Antibiotic susceptibility of ica-positive and ica-negative MRSA biofilms in different phases of biofilm growth. J Antibiot doi:10.1038/ja.2014.96
11. Daniel A, Euler C, Collin M, Chahales P, Gorelick KJ, Fischetti VA (2010) Synergism between a novel chimeric lysin and oxacillin protects against infection by methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother 54:1603–1612
12. Fischer A, Kambara K, Meyer H, Stenz L, Bonetti EJ, Girard M, Lalk M, Francois P, Schrenzel J (2014) GdpS contributes to Staphylococcus aureus biofilm formation by regulation of eDNA release. Int J Med Microbiol 304:284–299
13. Fischetti VA (2005) Bacteriophage lytic enzymes: novel anti infectives. Trends Microbiol 13(10):491–496
14. Fitzpatrick F, Humphreys H, O’Gara JP (2005) Evidence for icaADBC independent biofilm development mechanism in methicillin-resistant Staphylococcus aureus clinical isolates. J Clin Microbiol 43:1973–1976
15. Gu J, Xu W, Lei L, Huang J, Feng X, Sun C, Du C, Zuo J, Li Y, Du T, Li L, Han W (2011) LysGH15, a novel bacteriophage lysin, protects a murine bacteremia model efficiently against lethal methicillin-resistant Staphylococcus aureus infection. J Clin Microbiol 49(1):111–117
16. Hennig S, NyuntWai S, Ziebuhr W (2007) Spontaneous switch to PIA independent biofilm formation in an ica-positive Staphylococcus epidermidis isolate. Int J Med Microbiol 297:117–122
17. Houston P, Rowe SE, Pozzi C, Waters EM, O’Gara JP (2011) Essential role for the major autolysin in the fibronectin-binding protein-mediated Staphylococcus aureus biofilm phenotype. Infect Immun 79:1153–1165
18. Hughes KA, Sutherland IW, Clark J, Jones MV (1998) Biofilm susceptibility to bacteriophage attack: the role of phage-borne polysaccharide depolymerase. Microbiology 144:3039–3047
19. Ito A, Taniuchi A, May T, Kawata K, Okabe S (2009) Increased antibiotic resistance of Escherichia coli in mature biofilms. Appl Environ Microbiol 75:4093–4100
20. Jun SY, Jung GM, Son JS, Yoon SJ, Choi YJ, Kang SH (2011) Comparison of the antibacterial properties of phage endolysins SAL-1 and LysK. Antimicrob Agents Chemother 55(4):1764–1767
21. Ledala N, Wilkinson BJ, Jayaswal RK (2006) Effects of oxacillin and tetracycline on autolysis, autolysin processing and atl transcription in Staphylococcus aureus. Int J Antimicrob Agents 27:518–524
22. Leriche V, Sibille P, Carpentier B (2000) Use of an enzyme linked lectinosorbent assay to monitor the shift in polysachharide composition in bacterial biofilms. Appl Environ Microbiol 66:1851–1856
23. Loeffler JM, Nelson D, Fischetti VA (2001) Rapid killing of Streptococcus pneumoniae with a bacteriophage cell wall hydrolase. Science 294:2170–2172
24. Loessner MJ (2005) Bacteriophage endolysins—current state of research and applications. Curr Opin Microbiol 8(4):480–487
25. Loessner MJ, Kramer K, Ebel F, Scherer S (2002) C-terminal domains of Listeria monocytogenes bacteriophage murein hydrolases determine specific recognition and high-affinity binding to bacterial cell wall carbohydrates. Mol Microbiol 44:335–349
26. Lopez R, Garcıa E, Garcıa P (2004) Enzymes for anti-infective therapy: phage lysins. Drug Discov Today Ther Strateg 1(4):469–474
27. Miles AA, Misra SS, Irwin JO (1938) The estimation of the bactericidal power of the blood. J Hyg (Lond) 38:732–749
28. O’Flaherty S, Coffey A, Meaney W, Fitzgerald GF, Ross RP (2005) The recombinant phage lysinLysK has a broad spectrum of lytic activity against clinically relevant staphylococci, including methicillin-resistant Staphylococcus aureus. J Bacteriol 187:7161–7164
29. O’Neill E, Pozzi C, Houston P, Smyth D, Humphreys H, Robinson A, O’Gara JP (2007) Association between methicillin susceptibility and biofilm regulation in Staphylococcus aureus isolates from device-related infections. J Clin Microbiol 45:1379–1388
30. O’Neill E, Pozzi C, Houston P, Humphreys H, Robinson DA, Loughman A, Foster TJ, O’Gara JP (2008) A novel Staphylococcus aureus biofilm phenotype mediated by the fibronectin-binding proteins, FnBPA and FnBPB. J Bacteriol 190:3835–3850
31. Obeso JM, Martınez B, Rodrıguez A, Garcıa P (2008) Lytic activity of the recombinant staphylococcal bacteriophage phiH5 endolysin active against Staphylococcus aureus in milk. Int J Food Microbiol 128(2):211–218
32. Otto M (2008) Staphylococcal biofilms. Curr Top Microbiol Immunol 322:207–228
33. Pastagia M, Euler C, Chahales P, Fuentes-Duculan KJG, Fischetti VA (2011) A novel chimeric lysin shows superiority to mupirocin for skin decolonization of methicillin resistant and -sensitive Staphylococcus aureus strains. Antimicrob Agents Chemother 55(2):738–744
34. Pozzi C, Waters EM, Rudkin JK, Schaeffer CR, Lohan AJ, Tong P, Loftus BJ, Pier GB, Fey PD, Massey RC, O’Gara JP (2012) Methicillin resistance alters the biofilm phenotype and attenuates virulence in Staphylococcus aureus device-associated infections. PLoS Pathog 8(4):e1002626
35. Rani SA, Pitts B, Beyenal H, Veluchamy RA, Lewandowski Z, Davison WM, Buckingham-Meyer K, Stewart PS (2007) Spatial patterns of DNA replication, protein synthesis and oxygen concentration within bacterial biofilms reveal diverse physiological states. J Bacteriol 189:4223–4233
36. Rashel M, Uchiyama J, Ujihara T, Uehara Y, Kuramoto S, Sugihara S, Yagyu K, Muraoka A, Sugoi M, Hiramatsu K, Honke K, Matsuzaki S (2007) Efficient elimination of multidrug resistant Staphylococcus aureus by cloned lysin derived from bacteriophage ΦMR11. J Infect Dis 196:1237–1247
37. Sass P, Bierbaum G (2007) Lytic activity of recombinant bacteriophage phi11 and phi12 endolysins on whole cells and biofilms of Staphylococcus aureus. Appl Environ Microbiol 73:347–352
38. Schuch R, Nelson D, Fischetti VA (2002) A bacteriolytic agent that detects and kills Bacillus anthracis. Nature 418:884–889
39. Schuch R, Pelzek AJ, Raz A, Euler CW, Ryan PA, Winer BY, Farnsworth A, Bhaskaran SS, Stebblins CE, Xu Y, Clifford A, Bearss DJ, Vankayalapati H, Goldberg AR, Fischetti VA (2013) Use of a bacteriophage lysin to identify a novel target for antimicrobial development. PLoS ONE 8(4):e60754
40. Sedlacek MJ, Walker C (2007) Antibiotic resistance in an in vitro subgingival biofilm model. Oral Microbiol Immunol 22:333–339
41. Shen Y, Ko¨ller T, Kreikemeyer B, Nelson DC (2013) Rapid degradation of Streptococcus pyogenes biofilms by PlyC, a bacteriophage-encoded endolysin. J Antimicrob Chemother 68(8):1818–1824
42. Singh R, Ray P, Das A, Sharma M (2010) Enhanced production of exopolysaccharide matrix and biofilm by a menadione-auxotrophic Staphylococcus aureus small-colony variant. J Med Microbiol 59:521–527
43. Son JS, Lee SJ, Jun SY, Yoon SJ, Kang SH, Paik HR, Kang JO, Choi YJ (2010) Antibacterial and biofilm removal activity of a podoviridae Staphylococcus aureus bacteriophage SAP-2 and a derived recombinant cell-wall-degrading enzyme. Appl Microbiol Biotechnol 86(5):1439–1449
44. Vergara-Irigaray M, Maira-Litra´n T, Merino N, Pier GB, Lasa I (2008) Wall teichoic acids are dispensable for anchoring the PNAG exopolysaccharide to the Staphylococcus aureus cell surface. Microbiology 154:865–877
45. Vilain S, Pretorius JM, Theron J, Broz¨el VS (2009) DNA as an adhesin: Bacillus cereus requires extracellular DNA to form biofilms. Appl Environ Microbiol 75:2861–2868
46. Yamada S, Sugai M, Komatsuzawa H, Matsumato A (2001) Suppressed localisation of a major autolysin on Staphylococcus aureus treated with tetracycline. J Electron Microsc 50:359–364
47. Yokoi KJ, Kawahigashi N, Uchida M, Sugahara K, Shinohara M, Kawasaki KI (2005) The two-component cell lysis genes holWMY and lysWMY of the Staphylococcus warneri M phage WMY: cloning, sequencing, expression and mutational analysis in Escherichia coli. Gene 351:97–108
48. Yoong P, Schuch R, Nelson D, Fischetti VA (2004) Identification of a broadly active phage lytic enzyme with lethal activity against antibiotic-resistant Enterococcus faecalis and Enterococcus faecium. J Bacteriol 186(14):4808–4812