Enhanced expression of the codon-optimized exo-inulinase gene from the yeast Meyerozyma guilliermondii in Saccharomyces sp. W0 and bioethanol production from inulin

Applied Microbiology and Biotechnology

Volumn 98, Issue 21, 2014, Pages 9129-9138

  Liu, Guang Lei ^a   Fu, Ge Yi ^a   Chi, Zhe ^a   Chi, Zhen Ming ^a  

^a OCEAN UNIVERSITY OF CHINA   (China)

Author keywords

Codon optimization; Ethanol; Exo inulinase; Inulin; Meyerozyma guilliermondii; Saccharomyces sp. W0

Indexed keywords

BIOETHANOL; ETHANOL; POLYSACCHARIDES; PRODUCTIVITY; YEAST;

CODON OPTIMIZATION; INULIN; INULINASE; MEYEROZYMA GUILLIERMONDII; SACCHAROMYCES SP;

GENE EXPRESSION;

ALCOHOL; DNA FRAGMENT; FUNGAL DNA; GENOMIC DNA; INULIN; INULINASE; MESSENGER RNA; GLYCOSIDASE;

ALCOHOL PRODUCTION; ARTICLE; CODON; CODON USAGE; EXPRESSION VECTOR; FERMENTATION; FUNGAL GENE; GENE EXPRESSION; MEYEROZYMA GUILLIERMONDII; NONHUMAN; NUCLEOTIDE SEQUENCE; PRODUCTIVITY; PROTEIN SECONDARY STRUCTURE; REAL TIME POLYMERASE CHAIN REACTION; SACCHAROMYCES CEREVISIAE; CHEMISTRY; DNA SEQUENCE; ENZYMOLOGY; GENETICS; METABOLIC ENGINEERING; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; SACCHAROMYCETALES;

CLONING, MOLECULAR; DNA, FUNGAL; ETHANOL; GENE EXPRESSION; GLYCOSIDE HYDROLASES; INULIN; METABOLIC ENGINEERING; MOLECULAR SEQUENCE DATA; SACCHAROMYCETALES; SEQUENCE ANALYSIS, DNA;

EID: 84925487059     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-014-6079-7     Document Type: Article

References (36)

1. Angov E (2011) Codon usage: nature’s roadmap to expression and folding of proteins. Biotech J 6:650–659
2. Chi ZM, Liu ZR (1993) High-concentration alcoholic production from hydrolysate of raw ground corn by a tetraploid yeast strain. Biotechnol Lett 15:877–882
3. Chi ZM, Zhang T, Cao TS, Liu XY, Cui W, Zhao CH (2011) Biotechnological potential of inulin for bioprocesses. Biores Technol 102:4295–4303
4. Cui W, Wang Q, Zhang F, Zhang SC, Chi ZM, Madzak C (2011) Direct conversion of inulin into single cell protein by the engineered Yarrowia lipolytica carrying inulinase gene. Proc Biochem 46:1442–1448
5. Gietz RD, Woods RA (2006) Yeast transformation by the LiAc/SS Carrier DNA/PEG method. Meth Mol Biol 313:107–120
6. Gong F, Sheng J, Chi ZM, Li J (2007) Inulinase production by a marine yeast Pichia guilliermondii and inulin hydrolysis by the crude inulinase. J Ind Microbiol Biotechnol 34:179–185
7. Gong F, Chi ZM, Sheng J, Li J, Wang XH (2008) Purification and characterization of extracellular inulinase from a marine yeast Pichia guilliermondii and inulin hydrolysis by the purified inulinase. Biotechnol Bioproc Eng 13:533–539
8. Gustafsson C, Govindarajan S, Minshull J (2004) Codon bias and heterologous protein expression. Trends Biotechnol 22:346–353
9. He M, Wu D, Wu J, Chen J (2014) Enhanced expression of endoinulinase from Aspergillus niger by codon optimization in Pichia pastoris and its application in inulooligosaccharide production. J Ind Microbiol Biotechnol 41:105–114
10. Hu N, Yuan B, Sun J, Wang SA, Li FL (2012) Thermotolerant Kluyveromyces marxianus and Saccharomyces cerevisiae strains representing potentials for bioethanol production from Jerusalem artichoke by consolidated bioprocessing. Appl Microbiol Biotechnol 95:1359–1368
11. Kanaya S, Yamada Y, Kinouchi M, Kudo Y, Ikemura T (2001) Codon usage and tRNA genes in eukaryotes: correlation of codon usage diversity with translation efficiency and with CG-dinucleotide usage as assessed by multivariate analysis. J Mol Evol 53:290–298
12. Kudla G, Murray AW, Tollervey D, Plotkin JB (2009) Coding-sequence determinants of gene expression in Escherichia coli. Sci 324:255–258
13. Lane MM, Morrissey JP (2010) Kluyveromyces marxianus: a yeast emerging from its sister’s shadow. Fungal Biol Rev 24:17–26
14. Li Y, Liu GL, Chi ZM (2013) Ethanol production from inulin and unsterilized meal of Jerusalem artichoke tubers by Saccharomyces sp. W0 expressing the endo-inulinase gene from Arthrobacter sp. Biores Technol 147:254–259
15. Lim SH, Lee HW, Sok DE, Choi ES (2010) Recombinant production of an inulinase in a Saccharomyces cerevisiae gal80 strain. J Microbiol Biotechnol 20:1529–1533
16. Lim SH, Ryu JM, Lee HW, Jeon JH, Sok DE, Choi ES (2011) Ethanol fermentation from Jerusalem artichoke powder using Saccharomyces cerevisiae KCCM50549 without pretreatment for inulin hydrolysis. Biores Technol 102:2109–2111
17. Liu GL, Chi Z, Chi ZM (2013) Molecular characterization and expression of microbial inulinase genes. Crit Rev Microbiol 39:152–165
18. Liu X, Wu D, Wu J, Chen J (2012) Optimization of the production of Aspergillus niger α-glucosidase expressed in Pichia pastoris. World J Microb Biotechnol 29:533–540
19. Liu XY, Chi Z, Liu GL, Wang F, Madzak C, Chi ZM (2010) Inulin hydrolysis and citric acid production from inulin using the surface-engineered Yarrowia lipolytica displaying inulinase. Metab Eng 12:469–476
20. −ΔΔCT method. Meth 25:402–408
21. Mohr G, Hong W, Zhang J, Cui GZ, Yang Y, Cui QLYJ, Lambowitz AM (2013) A targetron system for gene targeting in thermophiles and its application in Clostridium thermocellum. PLoS ONE 8:e69032
22. Nevoigt E (2008) Progress in metabolic engineering of Saccharomyces cerevisiae. Microbiol Mol Biol Rev 72:379–412
23. Ostergaard S, Olsson L, Nielsen J (2000) Metabolic engineering of Saccharomyces cerevisiae. Microbiol Mol Biol Rev 64:34–50
24. Risso FV, Mazutti MA, Treichel H, Costa F, Maugeri F, Rodrigues MI (2010) METHODS: synthesis of fructooligosaccharides from sucrose in aqueous & aqueous-organic systems using free inulinase from Kluyveromyces marxianus ATCC 16045. J Ind Microbiol Biotechnol 6:288–294
25. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, pp 120–130
26. Saunders R, Deane CM (2010) Synonymous codon usage influences the local protein structure observed. Nucleic Acids Res 38:6719–6728
27. Spiro RG (1966) Analysis of sugars found in glycoproteins. Meth Enzymol 8:3–26
28. Wang GY, Chi Z, Song B, Wang ZP, Chi ZM (2012) High level lipid production by a novel inulinase-producing yeast Pichia guilliermondii Pcla22. Biores Technol 124:77–82
29. Wang JM, Zhang T, Chi Z, Liu GL, Chi ZM (2011) 18S rDNA integration of the exo-inulinase gene into chromosomes of the high ethanol producing yeast Saccharomyces sp. W0 for direct conversion of inulin to bioethanol. Biom Bioeng 35:3032–3039
30. Wang SA, Li FL (2013) Invertase SUC2 is the key hydrolase for inulin degradation in Saccharomyces cerevisiae. Appl Environ Microb 79:403–406
31. Wu CFJ, Hamada MS (2000) Experiments planning, analysis and parameter design optimization. John Wiley and Sons, New York, NY, pp 23–34
32. Yuan B, Wang SA, Li FL (2013a) Improved ethanol fermentation by heterologous endoinulinase and inherent invertase from inulin by Saccharomyces cerevisiae. Biores Technol 139:402–405
33. Yuan WJ, Chang BL, Ren JG, Liu JP, Bai FW, Li YY (2012) Consolidated bioprocessing strategy for ethanol production from Jerusalem artichoke tubers by Kluyveromyces marxianus under high gravity conditions. J Appl Microbiol 112(1):38–44
34. Yuan WJ, Li NN, Zhao XQ, Chen LJ, Kong L, Bai FW (2013b) Engineering an industrial Saccharomyces cerevisiae strain with the inulinase gene for more efficient ethanol production from Jerusalem artichoke tubers. Eng Life Sci 13:472–478
35. Zhang T, Chi Z, Chi ZM, Parrou JL, Gong F (2010a) Expression of the inulinase gene from the marine-derived Pichia guilliermondii in Saccharomyces sp. W0 and ethanol production from inulin. Microb Biotechnol 3:576–582
36. Zhang T, Chi Z, Zhao CH, Chi ZM, Gong F (2010b) Bioethanol production from hydrolysates of inulin and the tuber meal of Jerusalem artichoke by Saccharomyces sp. W0. Biores Technol 101:8166–8170