Immunohistochemical tools and techniques to visualize notch in Drosophila melanogaster

Methods in Molecular Biology

Volumn 1187, Issue , 2014, Pages 63-78

  Tognon, Emiliana ^a   Vaccari, Thomas ^a  

^a FIRC INSTITUTE OF MOLECULAR ONCOLOGY   (Italy)

Author keywords

Confocal microscopy; Drosophila melanogaster; Imaginal discs; Notch localization; Whole mount immunohistochemistry

Indexed keywords

FLUORESCENT DYE; MONOCLONAL ANTIBODY; NOTCH RECEPTOR; SCAVENGER; DROSOPHILA PROTEIN; NOTCH PROTEIN, DROSOPHILA;

ACTIN FILAMENT; ANTIBODY LABELING; ARTICLE; CELL STRUCTURE; CONFOCAL MICROSCOPY; DROSOPHILA MELANOGASTER; ENDOSOME; IMAGINAL DISC; IMMUNODETECTION; IMMUNOFLUORESCENCE TEST; IMMUNOHISTOCHEMISTRY; IMMUNOLOCALIZATION; MEMBRANE STRUCTURE; NONHUMAN; PRIORITY JOURNAL; PROTEIN LOCALIZATION; ANIMAL; CHEMISTRY; PROCEDURES; ULTRASTRUCTURE;

ANIMALS; DROSOPHILA MELANOGASTER; DROSOPHILA PROTEINS; IMMUNOHISTOCHEMISTRY; MICROSCOPY, CONFOCAL; RECEPTORS, NOTCH;

EID: 84925287036     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-1139-4_5     Document Type: Article

References (54)

1. Ramos-Vara JA (2005) Technical aspects of immunohistochemistry. Vet Pathol 42:405-426
2. Diederich RJ, Matsuno K, Hing H et al (1994) Cytosolic interaction between deltex and Notch ankyrin repeats implicates deltex in the Notch signaling pathway. Development 120:473-481
3. Fehon RG, Kooh PJ, Rebay I et al (1990) Molecular interactions between the protein products of the neurogenic loci Notch and Delta, two EGF-homologous genes in Drosophila. Cell 61:523-534
4. Vaccari T, Lu H, Kanwar R et al (2008) Endosomal entry regulates Notch receptor activation in Drosophila melanogaster. J Cell Biol 180:755-762
5. Wilkin MB, Carbery AM, Fostier M et al (2004) Regulation of notch endosomal sorting and signaling by Drosophila Nedd4 family proteins. Curr Biol 14:2237-2244
6. Guo Y, Livne-Bar I, Zhou L et al (1999) Drosophila presenilin is required for neuronal differentiation and affects notch subcellular localization and signaling. J Neurosci 19: 8435-8442
7. Ye Y, Fortini ME (1998) Characterization of Drosophila presenilin and its colocalization with Notch during development. Mech Dev 79:199-211
8. 8. Sakata T, Sakaguchi H, Tsuda L et al (2004) Drosophila Nedd4 regulates endocytosis of notch and suppresses its ligand-independent activation. Curr Biol 14:2228-2236
9. Couturier L, Vodovar N, Schweisguth F (2012) Endocytosis by Numb breaks Notch symmetry at cytokinesis. Nat Cell Biol 14: 131-139
10. Fortini ME, Bilder D (2009) Endocytic regulation of Notch signaling. Curr Opin Genet Dev 19:323-328
11. Purves DC, Brachmann C (2007) Dissection of imaginal discs from 3rd instar Drosophila larvae. J Vis Exp 2:140
12. Jauffred B, Bellaiche Y (2012) Analyzing frizzled signaling using fi xed and live imaging of the asymmetric cell division of the Drosophila sensory organ precursor cell. Methods Mol Biol 839:19-25
13. Zitserman D, Roegiers F (2011) Live-cell imaging of sensory organ precursor cells in intact Drosophila pupae. J Vis Exp 51:2706
14. Wong LC, Schedl P (2006) Dissection of Drosophila ovaries. J Vis Exp 1:52
15. Sullivan W, Ashburner M, Hawley S (2000) Drosophila protocols. Cold Spring Harbor Laboratory Press, New York
16. Barth JM, Hafen E, Köhler K (2012) The lack of autophagy triggers precocious activation of Notch signaling during Drosophila oogenesis. BMC Dev Biol 12:35
17. Daul AL, Komori H, Lee C-Y (2010) Immunofl uorescent staining of Drosophila larval brain tissue. Cold Spring Harb Protoc 2010(7):pdb.prot5460
18. Egger B, Gold KS, Brand AH (2010) Notch regulates the switch from symmetric to asymmetric neural stem cell division in the Drosophila optic lobe. Development 137:2981-2987
19. Kennison JA (2008) Dissection of larval salivary glands and polytene chromosome preparation. Cold Spring Harb Protoc 2008:pdb. prot4708
20. Kulkarni V, Khadilkar RJ, Magadi SS et al (2011) Asrij maintains the stem cell niche and controls differentiation during Drosophila lymph gland hematopoiesis. PLoS One 6: e27667
21. Lebestky T, Jung S-H, Banerjee U (2003) A serrate-expressing signaling center controls Drosophila hematopoiesis. Genes Dev 17: 348-353
22. Lloyd T, Atkinson R, Wu MN et al (2002) Hrs regulates endosome membrane invagination and tyrosine kinase receptor signaling in Drosophila. Cell 108:261-269
23. Vaccari T, Duchi S, Cortese K et al (2010) The vacuolar ATPase is required for physiological as well as pathological activation of the Notch receptor. Development 137:1825-1832
24. Lu H, Bilder D (2005) Endocytic control of epithelial polarity and proliferation in Drosophila. Nat Cell Biol 7:1232-1239
25. Yan Y, Denef N, Schüpbach T (2009) The vacuolar proton pump, V-ATPase, is required for Notch signaling and endosomal traffi cking in Drosophila. Dev Cell 17:387-402
26. Huppert SS, Jacobsen TL, Muskavitch MA (1997) Feedback regulation is central to Delta-Notch signalling required for Drosophila wing vein morphogenesis. Development 124: 3283-3291
27. Le Borgne R, Remaud S, Hamel S et al (2005) Two distinct E3 ubiquitin ligases have complementary functions in the regulation of delta and serrate signaling in Drosophila. PLoS Biol 3:e96
28. Thomas U, Speicher SA, Knust E (1991) The Drosophila gene Serrate encodes an EGF-like transmembrane protein with a complex expression pattern in embryos and wing discs. Development 111:749-761
29. Busseau I, Diederich RJ, Xu T et al (1994) A member of the Notch group of interacting loci, deltex encodes a cytoplasmic basic protein. Genetics 136:585-596
30. Matsuno K, Ito M, Hori K et al (2002) Involvement of a proline-rich motif and RING-H2 fi nger of Deltex in the regulation of Notch signaling. Development 129:1049-1059
31. Pitsouli C, Delidakis C (2005) The interplay between DSL proteins and ubiquitin ligases in Notch signaling. Development 132: 4041-4050
32. Daskalaki A, Shalaby NA, Kux K et al (2011) Distinct intracellular motifs of Delta mediate its ubiquitylation and activation by Mindbomb1 and Neuralized. J Cell Biol 195:1017-1031
33. Lai EC, Deblandre GA, Kintner C et al (2001) Drosophila neuralized is a ubiquitin ligase that promotes the internalization and degradation of delta. Dev Cell 1:783-794
34. Rhyu MS, Jan LY, Jan YN (1994) Asymmetric distribution of numb protein during division of the sensory organ precursor cell confers distinct fates to daughter cells. Cell 76:477-491
35. Jafar-Nejad H, Andrews HK, Acar M et al (2005) Sec15, a component of the exocyst, promotes Notch signaling during the asymmetric division of Drosophila sensory organ precursors. Dev Cell 9:351-363
36. O'Connor-Giles KM, Skeath JB (2003) Numb inhibits membrane localization of Sanpodo, a four-pass transmembrane protein, to promote asymmetric divisions in Drosophila. Dev Cell 5:231-243
37. Bettler D, Pearson S, Yedvobnick B (1996) The nuclear protein encoded by the Drosophila neurogenic gene mastermind is widely expressed and associates with specifi c chromosomal regions. Genetics 143:859-875
38. Helms W, Lee H, Ammerman M et al (1999) Engineered truncations in the Drosophila mastermind protein disrupt Notch pathway function. Dev Biol 215:358-374
39. Gho M, Lecourtois M, Géraud G et al (1996) Subcellular localization of suppressor of hairless in Drosophila sense organ cells during Notch signalling. Development 122:1673-1682
40. Jafar-Nejad H, Tien AC, Acar M et al (2006) Senseless and Daughterless confer neuronal identity to epithelial cells in the Drosophila wing margin. Development 133:1683-1692
41. Skeath JB, Carroll SB (1991) Regulation of achaete-scute gene expression and sensory organ pattern formation in the Drosophila wing. Genes Dev 5:984-995
42. Nolo R, Abbott LA, Bellen HJ (2000) Senseless, a Zn-fi nger transcription factor, is necessary and suffi cient for sensory organ development in Drosophila. Cell 102: 349-362
43. Simon F, Fichelson P, Gho M et al (2009) Notch and Prospero repress proliferation following cyclin E overexpression in the Drosophila bristle lineage. PLoS Genet 5: e1000594
44. Lee CY, Robinson KJ, Doe CQ (2006) Lgl, Pins and aPKC regulate neuroblast selfrenewal versus differentiation. Nature 439: 594-598
45. Vaccari T, Rabouille C, Ephrussi A (2005) The Drosophila PAR-1 spacer domain is required for lateral membrane association and for polarization of follicular epithelial cells. Curr Biol 15:255-261
46. Aoyama N, Yamakawa T, Sasamura T et al (2013) Loss-and gain-of-function analyses of vacuolar protein sorting 2 in Notch signaling of Drosophila melanogaster. Genes Genet Syst 88:45-57
47. Wucherpfennig T, Wilsch-Bräuninger M, González-Gaitán M (2003) Role of Drosophila Rab5 during endosomal traffi cking at the synapse and evoked neurotransmitter release. J Cell Biol 161:609-624
48. Bökel C, Schwabedissen A, Entchev E et al (2006) Sara endosomes and the maintenance of Dpp signaling levels across mitosis. Science 314:1135-1139
49. Tanaka T, Nakamura A (2008) The endocytic pathway acts downstream of Oskar in Drosophila germ plasm assembly. Development 135:1107-1117
50. Dollar G, Struckhoff E, Michaud J et al (2002) Rab11 polarization of the Drosophila oocyte: a novel link between membrane traffi cking, microtubule organization, and oskar mRNA localization and translation. Development 129:517-526
51. Wang W, Li Y, Zhou L et al (2011) Role of JAK/STAT signaling in neuroepithelial stem cell maintenance and proliferation in the Drosophila optic lobe. Biochem Biophys Res Commun 410:714-720
52. Sisson JC, Field C, Ventura R et al (2000) Lava Lamp, a novel peripheral golgi protein, is required for Drosophila melanogaster cellularization. J Cell Biol 151:905-918
53. Emery G, Hutterer A, Berdnik D et al (2005) Asymmetric Rab 11 endosomes regulate delta recycling and specify cell fate in the Drosophila nervous system. Cell 122:763-773
54. Mehta SQ, Hiesinger PR, Beronja S et al (2005) Mutations in Drosophila sec15 reveal a function in neuronal targeting for a subset of exocyst components. Neuron 46:219-232