In vitro models that utilize hypoxia to induce non-replicating persistence in mycobacteria

Methods in Molecular Biology

Volumn 1285, Issue , 2015, Pages 201-213

  Sohaskey, Charles D ^a   Voskuil, Martin I ^a  

^a NONE

Author keywords

Dormancy; Hypoxia; Latent infection; Mycobacterium tuberculosis; Persistence; Tuberculosis

Indexed keywords

ADENOSINE TRIPHOSPHATE; NICOTINAMIDE ADENINE DINUCLEOTIDE; NITRATE; NITRATE REDUCTASE; NITRITE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE; RNA; NITRIC ACID DERIVATIVE;

ANAEROBIC BACTERIUM; ANAEROBIC GROWTH; ARTICLE; BACILLI; BACTERIAL GROWTH; CELL COUNT; COLONY FORMING UNIT; ENZYME ACTIVITY; HYPOXIA; IN VITRO STUDY; INCUBATION TIME; MYCOBACTERIUM BOVIS; MYCOBACTERIUM SMEGMATIS; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; OPTICAL DENSITY; PRIORITY JOURNAL; PROTEIN BINDING; RNA ISOLATION; ANOXIA; BACTERIAL COUNT; GENETICS; GROWTH, DEVELOPMENT AND AGING; METABOLISM; MYCOBACTERIUM;

BACILLI (CLASS); CORYNEBACTERINEAE; MYCOBACTERIUM TUBERCULOSIS;

ADENOSINE TRIPHOSPHATE; ANAEROBIOSIS; ANOXIA; COLONY COUNT, MICROBIAL; IN VITRO TECHNIQUES; MYCOBACTERIUM; MYCOBACTERIUM TUBERCULOSIS; NAD; NITRATES; NITRITES;

EID: 84925276044     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-2450-9_11     Document Type: Article

References (17)

1. Wayne LG, Sohaskey CD (2001) Nonreplicating persistence of Mycobacterium tuberculosis. Annu Rev Microbiol 55:139–163.
2. Via LE, Lin PL, Ray SM, Carrillo J, Allen SS, Eum SY, Taylor K, Klein E, Manjunatha U, Gonzales J, Lee EG, Park SK, Raleigh JA, Cho SN, McMurray DN, Flynn JL, Barry CEI (2008) Tuberculous granulomas are hypoxic in guinea pigs, rabbits, and nonhuman primates. Infect Immun 76:2332–2340.
3. Wayne LG, Hayes LG (1996) An in vitro model for sequential study of shiftdown of Mycobacterium tuberculosis through two stages of non-replicating persistence. Infect Immun 64:2062–2069.
4. Watanabe S, Zimmermann M, Goodwin MB, Sauer U, Barry CEI, Boshoff HI (2011) Fumarate reductase activity maintains an energized membrane in anaerobic Mycobacterium tuberculosis. PLoS Pathog 7:1–15.
5. Voskuil MI, Schnappinger D, Harrell MI, Visconti KC, Dolganov GM, Sherman DR, Schoolnik GK, (2003) Inhibition of respiration by nitric oxide induces a Mycobacterium tuberculosis persistence program. J Exp Med 198:705–713.
6. Sohaskey CD, Wayne LG (2003) Role of narK2X and narGHJI in hypoxic upregulation of nitrate reduction by Mycobacterium tuberculosis. J Bacteriol 185:7247–7256.
7. Wayne LG, Hayes LG (1999) Nitrate reduction as a marker for hypoxic shiftdown of Mycobacterium tuberculosis. Tuber Lung Dis 79:127–132.
8. Leistikow RL, Morton RA, Bartek IL, Frimpong I, Wagner K, Voskuil MI (2010) The Mycobacterium tuberculosis DosR regulon assists in metabolic homeostasis and enables rapid recovery from nonrespiring dormancy. J Bacteriol 192:1662–1670.
9. Boshoff HIM, Myers TG, Copp BR, McNeil MR, Wilson MA, Barry CEI (2004) The transcriptional responses of Mycobacterium tuberculosis to inhibitors of metabolism. J Biol Chem 279:40174–40184.
10. Wayne LG (1977) Synchronized replication of Mycobacterium tuberculosis. Infect Immun 17:528–530.
11. Bartek IL, Woolhiser LK, Baughn AD, Basaraba RJ, Jacobs WRJ, Lenaerts AJ, Voskuil MI (2014) Mycobacterium tuberculosis Lsr2 is a global transcriptional regulator required for adaptation to changing oxygen levels and virulence. MBio 5:e01106–e01114.
12. Shi L, Sohaskey CD, Pfeiffer C, Parks M, McFadden J, North RJ, Gennaro ML (2010) Carbon fl ux rerouting during Mycobacterium tuberculosis growth arrest. Mol Microbiol 78:1199–1215.
13. Sohaskey CD, Modesti L (2009) Differences in nitrate reduction between Mycobacterium tuberculosis and Mycobacterium bovis are due to differential expression of both narGHJI and narK2. FEMS Microbiol Lett 290:129–134.
14. Honaker RW, Stewart A, Schittone S, Izzo A, Klein MR, Voskuil MI (2008) BCG vaccine strains lack narK2 and narX induction and exhibit altered phenotypes during dormancy. Infect Immun 76:2587–2593.
15. Lim A, Eleuterio M, Hutter B, Murugasu-Oei B, Dick T (1999) Oxygen depletion induced dormancy in Mycobacterium bovis BCG. J Bacteriol 181:2252–2256.
16. Dick T, Lee BH, Murugasu-Oei B (1998) Oxygen depletion induced dormancy in Mycobacterium smegmatis. FEMS Microbiol Lett 163:159–164.
17. London J, Knight M (1966) Concentrations of nicotinamide nucleotide coenzymes in micro- organisms. J Gen Microbiol 44:254.