Protected hinge in the immunoglobulin G2-A2 disulfide isoform

Protein Science

Volumn 23, Issue 12, 2014, Pages 1753-1764

  Liu, Yaoqing Diana ^a   Chou, Robert Y T ^a   Dillon, Thomas M ^a   Poppe, Leszek ^a   Spahr, Chris ^a   Shi, Stone D H ^a   Flynn, Gregory C ^a  

^a AMGEN INC   (United States)

Author keywords

disulfide reduction; monoclonal antibodies; NMR; protein dynamics

Indexed keywords

CYSTEINE; DISULFIDE; GLOBULAR PROTEIN; GLYCAN; IMMUNOGLOBULIN G2; IMMUNOGLOBULIN G2 A2; IMMUNOGLOBULIN G2B; UNCLASSIFIED DRUG; IMMUNOGLOBULIN G; ISOPROTEIN; RECOMBINANT PROTEIN;

ANIMAL CELL; ARTICLE; DISULFIDE BOND; FEMALE; HINGE REGION; MUTAGENESIS; NONHUMAN; PEPTIDE MAPPING; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTON NUCLEAR MAGNETIC RESONANCE; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; CHEMISTRY; HUMAN; NUCLEAR MAGNETIC RESONANCE;

DISULFIDES; HUMANS; IMMUNOGLOBULIN G; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PROTEIN ISOFORMS; RECOMBINANT PROTEINS;

EID: 84925259330     PISSN: 09618368     EISSN: 1469896X     Source Type: Journal    
DOI: 10.1002/pro.2557     Document Type: Article

References (12)

1. Wypych J, Li M, Guo A, Zhang Z, Martinez T, Allen MJ, Fodor S, Kelner DN, Flynn GC, Liu YD, Bondarenko PV, Ricci MS, Dillon TM, Balland A, (2008) Human IGG2 antibodies display disulfide mediated structural isoforms. J Biol Chem 283: 16194-16205.
2. Dillon TM, Ricci MS, Vezina C, Flynn GC, Liu YD, Rehder DS, Plant M, Henkle B, Li Y, Deechongkit S, Varnum B, Wypych J, Balland A, Bondarenko PV, (2008) Structural and functional characterization of disulfide isoforms of the human IGG2 subclass. J Biol Chem 283: 16206.
3. Zhang B, Harder AG, Connelly HM, Maheu LL, Cockrill SL, (2010) Determination of Fab-hinge disulfide connectivity in structural isoforms of a recombinant human immunoglobulin G2 antibody. Anal Chem 82: 1090-1099.
4. Liu YD, Chen X, Enk JZ, Plant M, Dillon TM, Flynn GC, (2008) Human IgG2 antibody disulfide rearrangement in vivo. J Biol Chem 283: 29266-29272.
5. Liu YD, Wang T, Chou R, Chen L, Kannan G, Stevenson R, Goetze AM, Jiang XG, Huang G, Dillon TD, Flynn GC, (2013) IgG2 disulfide isoform conversion kinetics. Mol Immunol 54: 217-226.
6. Yan B, Eris T, Yates Z, Hong RW, Steen S, Kleemann G, Wang W, Liu JL, (2009) Analysis of human antibody IgG2 domains by reversed-phase liquid chromatography and mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci 877: 1613-1620.
7. Poppe L, Jordan JJ, Lawson K, Jerums M, Apostol I, Schnier P, (2013) Profiling formulated monoclonal antibodies by 1H NMR spectroscopy. Anal Chem 85: 9623-9629.
8. Feige MJ, Groscurth S, Marcinowski M, Shimizu Y, Kessler H, Hendershot LM, Buchner J, (2009) An unfolded CH1 domain controls the assembly and secretion of IgG antibodies. Mol Cell 34: 569-579.
9. Shukla AA, Hubbard B, Tressel T, Guhan S, Low D, (2007) Downstream processing of monoclonal antibodies-application of platform approaches. J Chromatogr B Analyt Technol Biomed Life Sci 848: 28-39.
10. Chen X, Tang K, Lee M, Flynn GC, (2008) Microchip assays for screening monoclonal antibody product quality. Electrophoresis 29: 4993-5002.
11. Jiang XG, Wang T, Kaltenbrunner O, Chen K, Flynn GC, Huang G, (2013) Evaluation of protein disulfide conversion in vitro using a continuous flow dialysis system. Anal Biochem 432: 142-154.
12. Grant GA, Crankshaw MW, (2003) Identification of PTH-amino acids by HPLC. Methods Mol Biol 211: 247-268.