Analysis of human antibody IgG2 domains by reversed-phase liquid chromatography and mass spectrometry

Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

Volumn 877, Issue 16-17, 2009, Pages 1613-1620

  Yan, Boxu ^a   Eris, Tamer ^b   Yates, Zac ^a   Hong, Robert W ^b   Steen, Sean ^a   Kleemann, Gerd ^a   Wang, Weichun ^a   Liu, Jennifer L ^b  

^a AMGEN INC   (United States)

^b AMGEN INC   (United States)

Author keywords

Antibody IgG2; Hinge; Mass spectrometry; Modification; Papain; RP HPLC

Indexed keywords

HEAVY CHAINS; HUMAN ANTIBODIES; IN LINES; LC-MS/MS; LIGHT CHAINS; MODIFICATION; NEW STRATEGIES; PAPAIN; PAPAIN CLEAVAGES; PEPTIDE MAPPINGS; REDUCING CONDITIONS; RETENTION TIME; REVERSED-PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHIES; REVERSED-PHASE LIQUID CHROMATOGRAPHIES; RP-HPLC; SINGLE CHAINS; TIME-OF FLIGHT MASS SPECTROMETRIES;

AMINES; CHROMATOGRAPHIC ANALYSIS; CHROMATOGRAPHY; HIGH PRESSURE LIQUID CHROMATOGRAPHY; LIQUIDS; MASS SPECTROMETERS; MASS SPECTROMETRY; MOLECULES; MONOCLONAL ANTIBODIES; PINCH EFFECT; SPECTRUM ANALYSIS;

HIGH PERFORMANCE LIQUID CHROMATOGRAPHY;

IMMUNOGLOBULIN ANTIBODY; IMMUNOGLOBULIN FC FRAGMENT; IMMUNOGLOBULIN G1; IMMUNOGLOBULIN G2; MONOCLONAL ANTIBODY; PAPAIN;

ANTIBODY DETECTION; ARTICLE; HEAVY CHAIN; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; LIGHT CHAIN; MASS SPECTROMETRY; MOLECULE; MULTIPLE SCLEROSIS; PEPTIDE MAPPING; PRIORITY JOURNAL; REVERSED PHASE LIQUID CHROMATOGRAPHY; SEPARATION TECHNIQUE; TIME OF FLIGHT MASS SPECTROMETRY;

CHROMATOGRAPHY, LIQUID; HUMANS; IMMUNOGLOBULIN G; PROTEIN STRUCTURE, TERTIARY; TANDEM MASS SPECTROMETRY;

EID: 65549128059     PISSN: 15700232     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jchromb.2009.03.043     Document Type: Article

References (22)

1. Porter R. Biochem. J. 73 (1959) 119
2. Utsumi S. Biochem. J. 112 (1969) 343
3. Parham P., Androlewicz M.J., Brodsky F.M., Holmes N.J., and Ways J.P. J. Immunol. Methods 53 (1982) 133
4. Pokkuluri P.R., Bouthillier F., Li Y., Kuderova A., Lee J., and Cygler M. J. Mol. Biol. 243 (1994) 283
5. Dainiak M.B., Muronetz V.I., Izumrudov V.A., Galaev I.Y., and Mattiasson B. Anal. Biochem. 277 (2000) 58
6. Akita E.M., and Nakai S. J. Immunol. Methods 162 (1993) 155
7. Coleman L., and Mahler S. Protein Expr. Purif. 32 (2003) 246
8. Moorhouse K.G., Nashabeh W., Deveney J., Bjork N.S., Mulkerrin M.G., and Ryskamp T. J. Pharm. Biomed. Anal. 16 (1997) 593
9. Yan B., Valliere-Douglass J., Brady L., Steen S., Han M., Pace D., Elliott S., Yates Z., Balland A., Wang W., and Pettit D. J. Chromatogr. A 1164 (2007) 153
10. Kleemann G., Beierle J., Nichols A., Dillon T., Pipes G., and Bondarenko P. Anal. Chem. 80 (2008) 2001
11. Matsumiya S., Yamaguchi Y., Saito J., Nagano M., Sasakawa H., Otaki S., Satoh M., Shitara K., and Kato K. J. Mol. Biol. 368 (2007) 767
12. Arnold J.N., Wormald M.R., Sim R.B., Rudd P.M., and Dwek R.A. Annu. Rev. Immunol. 25 (2007) 21
13. Walsh G., and Jefferis R. Nat. Biotechnol. 24 (2006) 1241
14. Dillon T.M., Ricci M.S., Vezina C., Flynn G., Liu Y., Rehder D., Plant M., Henkle M., Li Y., Deechongkit S., Varnum B., Wypych J., Balland A., and Bondarenko P. J. Biol. Chem. 283 (2008) 16206
15. Wypych J., Li M., Guo A., Zhang Z., Martinez T., Allen M., Fodor S., Kelner D., Flynn G., Liu Y., Bondarenko P., Ricci M.S., Dillon T.M., and Balland A. J. Biol. Chem. 283 (2008) 16194
16. Boyd J., Easterbrook-Smith S.B., Zavodszky P., Mountford-Wright C., and Dwek R.A. Mol. Immunol. 16 (1979) 851
17. Bigelow C.C., Smith E.R., and Dorrington K.J. Biochemistry 13 (1978) 4602
18. Horne C., Klein M., Polidoulis I., and Dorrington K. J. Immunol. 129 (1982) 660
19. Zhang Z. Anal. Chem. 76 (2004) 3908
20. Virella G., and Parkhouse G.R. Immunochemistry 10 (1973) 213
21. Lagerwerf F.M., Weert M., Heema W., and Haverkamp J. J. Rapid Commun. Mass Spectrom. 10 (1996) 1905
22. Bloom J.W., Madanat M.S., Marriott T., and Chan S.Y. Protein Sci. 6 (1997) 407