MiRNA in situ hybridization in circulating tumor cells - MishCTC

Scientific Reports

Volumn 5, Issue , 2015, Pages

  Ortega, Francisco G ^a   Lorente, Jose A ^a   Garcia Puche, Jose L ^a   Ruiz, Maria P ^a   Sanchez Martin, Rosario M ^a   De Miguel Pérez, Diego ^a   Diaz Mochon, Juan J ^a   Serrano, Maria J ^a  

^a UNIVERSITY OF GRANADA   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

MICRORNA; MIRN21 MICRORNA, HUMAN;

BLOOD; EPITHELIAL MESENCHYMAL TRANSITION; GENETICS; HUMAN; IN SITU HYBRIDIZATION; MCF 7 CELL LINE; METABOLISM; NEOPLASM; PATHOLOGY; PROCEDURES; REAL TIME POLYMERASE CHAIN REACTION; TUMOR CELL LINE; TUMOR EMBOLISM;

CELL LINE, TUMOR; EPITHELIAL-MESENCHYMAL TRANSITION; HUMANS; IN SITU HYBRIDIZATION; MCF-7 CELLS; MICRORNAS; NEOPLASMS; NEOPLASTIC CELLS, CIRCULATING; REAL-TIME POLYMERASE CHAIN REACTION;

EID: 84924973763     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep09207     Document Type: Article

References (23)

1. Mehlen, P. & Puisieux, A.Metastasis: a question of life or death. Nat. Rev. Cancer 6, 449-458 (2006).
2. Nadal, R., Lorente, J. A., Rosell, R. & Serrano M. J. Relevance of molecular characterization of circulating tumor cells in breast cancer in the era of targeted therapies. Expert Rev. Mol. Diagn. 13, 295-307 (2013).
3. Cristofanilli, M. et al. Circulating Tumor Cells, Disease Progression, and Survival in Metastatic Breast Cancer. New Engl. J. Med. 351, 781-791 (2004).
4. Schulze, K. et al. Presence of EpCAM-positive circulating tumor cells as biomarker for systemic disease strongly correlates to survival in patients with hepatocellular carcinoma. Int. J. Cancer. 133, 2165-2171 (2013).
5. Zhao, S. et al. Circulating tumor cells (CTCs) detected by triple-marker EpCAM, CK19, and hMAM RT-PCR and their relation to clinical outcome in metastatic breast cancer patients. Cell Biochem. Biophys. 65, 263-273 (2013).
6. Nadal, R. et al. Biomarkers characterization of circulating tumour cells in breast cancer patients. Breast Cancer Res. 14: R71 (2012).
7. Riethdorf, S. et al. Detection of circulating tumor cells in peripheral blood of patients with metastatic breast cancer: a validation study of the CellSearch system. Clin. Cancer Res. 13, 920-928 (2007).
8. Saucedo-Zeni, N. et al. A novel method for the in vivo isolation of circulating tumor cells from peripheral blood of cancer patients using a functionalized and structured medical wire. Int. J. Oncol. 41, 1241-1250 (2012).
9. Yu, M. et al. Circulating breast tumor cells exhibit dynamic changes in epithelial and mesenchymal composition. Science 339, 580-584 (2013).
10. Krebs, M. G. et al. Molecular analysis of circulating tumour cells-biology and biomarkers. Nat. Rev. Clin. Oncol. 11, 129-144 (2014).
11. Croce, C. M. Causes and consequences of miRNA dysregulation in cancer. Nat. Rev. Genet. 10, 704-714 (2009).
12. Godfrey, A. C. Serum miRNA expression as an early marker for breast cancer risk in prospectively collected samples from the Sister Study cohort. Breast Cancer Res. 15, R42 (2013).
13. Tricoli, J. V. & Jacobson, J. W. MiRNA: Potential for Cancer Detection, Diagnosis, and Prognosis. Cancer Res. 67, 4553-4555 (2007).
14. Madhavan, D. Circulating miRNAs as surrogate markers for circulating tumor cells and prognostic markers in metastatic breast cancer. Clin. Cancer Res. 18, 5972-5982 (2012).
15. Sieuwerts, A. M. et al.mRNAand miRNA expression profiles in circulating tumor cells and primary tumors of metastatic breast cancer patients. Clin. Cancer Res. 17, 3600-3618 (2011).
16. Pena, J. T. et al. miRNA in situ hybridization in formaldehyde and EDC-fixed tissues. Nat. Methods 6, 139-141 (2009).
17. Paragas, V. B., Zhang, Y. Z., Haugland, R. P. & Singer, V. L. The ELF-97 alkaline phosphatase substrate provides a bright, photostable, fluorescent signal amplification method for FISH. Histochem. Cytochem. 45, 345-357 (1997).
18. Lu, J. & Tsourkas, A. Imaging individual miRNAs in single mammalian cells in situ. Nucleic Acids Res. 37, e100 (2009).
19. Li, Z. F. Dynamic localisation of mature miRNAs in Human nucleoli is influenced by exogenous genetic materials. PLoS ONE 8, e70869 (2013).
20. Medina, P., Nolde, M. & Slack, F. J. OncomiR addiction in an in vivo model of microRNA-21-induced pre-B-cell lymphoma. Nature 467, 86-90 (2010).
21. Serrano, M. J. et al. EMT and EGFR in CTCs cytokeratin negative non-metastatic breast cancer. Oncotarget 5, 7486-7497 (2014).
22. Bornachea, O. et al. EMT and induction of miR21 mediate metastasis development in Trp53-deficient tumours. Sci Rep. 2, 434-445 (2012).
23. Mitsuhiro, Y. et al. The increase of microRNA-21 during lung fibrosis and its contribution to epithelial-mesenchymal transition in pulmonary epithelial cells. Respir. Res. 14, 95-107 (2013).