Analysis of microRNA niches: Techniques to measure extracellular microRNA and intracellular microRNA in situ

Methods in Molecular Biology

Volumn 1024, Issue , 2013, Pages 157-172

  Parikh, Victoria N ^a   Chan, Stephen Y ^b  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b BRIGHAM AND WOMEN S HOSPITAL   (United States)

Author keywords

Circulating miRNA; In situ staining; qRT PCR; RNA extraction; Serum biomarkers

Indexed keywords

FORMALDEHYDE; MICRORNA; PARAFFIN; BIOLOGICAL MARKER; CULTURE MEDIUM; DIAGNOSTIC KIT; FIXATIVE;

ANIMAL CELL; ARTICLE; CULTURE MEDIUM; HUMAN; HUMAN CELL; NONHUMAN; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS; RNA EXTRACTION; ULTRACENTRIFUGATION; CHEMISTRY; CYTOLOGY; DIAGNOSTIC KIT; ENDOTHELIUM CELL; EXOSOME; EXTRACELLULAR SPACE; HISTOLOGY; IMMUNOPRECIPITATION; ISOLATION AND PURIFICATION; METABOLISM; PULMONARY ARTERY; REAL TIME POLYMERASE CHAIN REACTION; SMOOTH MUSCLE FIBER; STAINING;

BIOLOGICAL MARKERS; CULTURE MEDIA, CONDITIONED; ENDOTHELIAL CELLS; EXOSOMES; EXTRACELLULAR SPACE; FIXATIVES; FORMALDEHYDE; HUMANS; IMMUNOPRECIPITATION; MICRORNAS; MYOCYTES, SMOOTH MUSCLE; PULMONARY ARTERY; REAGENT KITS, DIAGNOSTIC; REAL-TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; STAINING AND LABELING; TISSUE EMBEDDING; ULTRACENTRIFUGATION;

EID: 84924847836     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-453-1_12     Document Type: Article

References (13)

1. Mitchell PS, Parkin RK, Kroh EM et al (2008) Circulating microRNAs as stable blood-based markers for cancer detection. Proc Natl Acad Sci U S A 105:10513-10518
2. Baggish AL, Hale A, Weiner RB et al (2011) Dynamic regulation of circulating microRNA during acute exhaustive exercise and sustained aerobic exercise training. J Physiol 589: 3983-3994
3. Etheridge A, Lee I, Hood L et al (2011) Extracellular microRNAL a new source of biomarkers. Mutat Res 717:85-90
4. Kirschner MB, Kao SC, Edelman JJ et al (2011) Haemolysis during sample preparation alters microRNA content of plasma. PLoS One 6:e24145
5. McDonald JS, Milosevic D, Reddi HV et al (2011) Analysis of circulating microRNA: Preanalytical and analytical challenges. Clin Chem 57:833-840
6. Pritchard CC, Kroh E, Wood B et al (2011) Blood cell origin of circulating microRNAs: A cautionary note for cancer biomarker studies. Cancer Prev Res (Phila) 5: 492-497
7. Pena JT, Sohn-Lee C, Rouhanifard SH et al (2009) miRNA in situ hybridization in formaldehyde and EDC- fixed tissues. Nat Methods 6:139-141
8. Chau BN, Xin C, Hartner J et al (2012) MicroRNA-21 promotes fibrosis of the kidney by silencing metabolic pathways. Sci Transl Med 4:121ra18
9. Ho AS, Huang X, Cao H et al (2010) Circulating miR-210 as a novel hypoxia marker in pancreatic cancer. Transl Oncol 3:109-113
10. Parikh VN, Jin RC, Rabello S et al (2012) MicroRNA-21 integrates pathogenic signaling to control pulmonary hypertension: Results of a network bioinformatics approach. Circulation 125:1520-1532
11. Johnson ML, Navanukraw C, Grazul-Bilska AT et al (2003) Heparinase treatment of RNA before quantitative real-time RT-PCR. Biotechniques 35:1140-1142, 1144
12. Arroyo JD, Chevillet JR, Kroh EM et al (2011) Argonaute2 complexes carry a population of circulating microRNAs independent of vesicles in human plasma. Proc Natl Acad Sci USA 108:5003-5008
13. Kosaka N, Iguchi H, Yoshioka Y et al (2010) Secretory mechanisms and intercellular transfer of microRNAs in living cells. J Biol Chem 285:17442-17452