High-efficiency expression of TAT-bFGF fusion protein in Escherichia coli and the effect on hypertrophic scar tissue

PLoS ONE

Volumn 10, Issue 2, 2015, Pages

  Jia, Xuechao ^a   Tian, Haishan ^a   Tang, Lu ^a   Zheng, Long ^a   Zheng, Lulu ^a   Yang, Ting ^a   Yu, Bingjie ^a   Wang, Zhitao ^a   Lin, Peng ^a   Li, Xiaokun ^a   Wang, Xiaojie ^a  

^a WENZHOU MEDICAL UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

FIBROBLAST GROWTH FACTOR 2; HYBRID PROTEIN; TRANSACTIVATOR PROTEIN;

3T3 CELL LINE; AFFINITY CHROMATOGRAPHY; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; BACTERIAL STRAIN; BFGF GENE; CELL PROLIFERATION; CONTROLLED STUDY; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE EXPRESSION; GENE FUSION; HUMAN; HUMAN CELL; HYPERTROPHIC SCAR; IN VITRO STUDY; MALE; MOUSE; MTT ASSAY; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN PURIFICATION; RABBIT; SKIN PENETRATION; TAT GENE; ANIMAL; BAGG ALBINO MOUSE; CICATRIX, HYPERTROPHIC; DISEASE MODEL; GENETICS;

ANIMALIA; ESCHERICHIA COLI;

ANIMALS; CICATRIX, HYPERTROPHIC; DISEASE MODELS, ANIMAL; ESCHERICHIA COLI; FIBROBLAST GROWTH FACTOR 2; GENE PRODUCTS, TAT; MICE; MICE, INBRED BALB C; RABBITS; RECOMBINANT FUSION PROTEINS;

EID: 84923827164     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0117448     Document Type: Article

References (36)

1. Gospodarowicz D (1974) Localisation of a fibroblast growth factor and its effect alone and with hydrocortisone on 3T3 cell growth. Nature 249: 123-127. PMID: 4364816
2. Abraham JA, Whang JL, Tumolo A, Mergia A, Friedman J, et al. (1986) Human basic fibroblast growth factor: nucleotide sequence and genomic organization. Embo J 5: 2523-2528. PMID: 3780670
3. Gasparian ME, Elistratov PA, Drize NI, Nifontova IN, Dolgikh DA, et al. (2009) Overexpression in Escherichia coli and purification of human fibroblast growth factor (FGF-2). Biochemistry 74: 221-225. PMID: 19267679
4. Mu X, Kong N, Chen W, Zhang T, Shen M, et al. (2008) High-level expression, purification, and characterization of recombinant human basic fibroblast growth factor in Pichia pastoris. Protein Expr Purif 59: 282-288. doi: 10.1016/j.pep.2008.02.009 PMID: 18378165
5. Wu X, Kamei K, Sato H, Sato SI, Takano R, et al. (2001) High-level expression of human acidic fibroblast growth factor and basic fibroblast growth factor in silkworm (Bombyx mori L.) using recombinant baculovirus. Protein Expr Purif 21: 192-200. PMID: 11162406
6. Andres C, Hasenauer J, Ahn HS, Joseph EK, Isensee J, et al. (2013) Wound-healing growth factor, basic FGF, induces Erk1/2-dependent mechanical hyperalgesia. Pain 154: 2216-2226. doi: 10.1016/j.pain.2013.07.005 PMID: 23867734
7. Abe K, Saito H (2001) Effects of basic fibroblast growth factor on central nervous system functions. Pharmacol Res 43: 307-312. PMID: 11352534
8. Sgadari C, Barillari G, Palladino C, Bellino S, Taddeo B, et al. (2011) Fibroblast Growth Factor-2 and the HIV-1 Tat Protein Synergize in Promoting Bcl-2 Expression and Preventing Endothelial Cell Apoptosis: Implications for the Pathogenesis of AIDS-Associated Kaposi's Sarcoma. Int J Vasc Med 452729: 9. doi: 10.1155/2011/452729 PMID: 22007303
9. Ono I, Akasaka Y, Kikuchi R, Sakemoto A, Kamiya T, et al. (2007) Basic fibroblast growth factor reduces scar formation in acute incisional wounds. Wound Repair Regen 15: 617-623. PMID: 17971006
10. Burd A, Huang L (2005) Hypertrophic response and keloid diathesis: two very different forms of scar. Plast Reconstr Surg 116: 150e-157e. PMID: 16327593
11. Miller MC, Nanchahal J (2005) Advances in the modulation of cutaneous wound healing and scarring. BioDrugs 19: 363-381. PMID: 16392889
12. Xie J, Qi S, Xu Y, Tang J, Li T, et al. (2008) Effects of basic fibroblast growth factors on hypertrophic scarring in a rabbit ear model. J Cutan Med Surg 12: 155-162. PMID: 18627695
13. Koren E, Torchilin VP (2012) Cell-penetrating peptides: breaking through to the other side. Trends Mol Med 18: 385-393. doi: 10.1016/j.molmed.2012.04.012 PMID: 22682515
14. Nasrollahi SA, Taghibiglou C, Azizi E, Farboud ES (2012) Cell-penetrating peptides as a novel transdermal drug delivery system. Chem Biol Drug Des 80: 639-646. doi: 10.1111/cbdd.12008 PMID: 22846609
15. Kim DW, Eum WS, Jang SH, Yoon CS, Choi HS, et al. (2003) Ginsenosides enhance the transduction of tat-superoxide dismutase into mammalian cells and skin. Mol Cells 16: 402-406. PMID: 14744033
16. Lim JM, Chang MY, Park SG, Kang NG, Song YS, et al. (2003) Penetration enhancement in mouse skin and lipolysis in adipocytes by TAT-GKH, a new cosmetic ingredient. J Cosmet Sci 54: 483-491. PMID: 14605689
17. Rabiskova M, Sedlakova M, Vitkova M, Kuna M (2004) [Carbomers and their use in pharmaceutical technology]. Ceska Slov Farm 53: 300-303. PMID: 15630996
18. Morris DE, Wu L, Zhao LL, Bolton L, Roth SI, et al. (1997) Acute and chronic animal models for excessive dermal scarring: quantitative studies. Plast Reconstr Surg 100: 674-681. PMID: 9283567
19. Kwong KW, Wong WK (2013) A revolutionary approach facilitating co-expression of authentic human epidermal growth factor and basic fibroblast growth factor in both cytoplasm and culture medium of Escherichia coli. Appl Microbiol Biotechnol 97: 9071-9080. doi: 10.1007/s00253-013-5090-8 PMID: 23880874
20. Nuc P, Nuc K (2006) [Recombinant protein production in Escherichia coli]. Postepy Biochem 52: 448-456. PMID: 17536514
21. Song L, Huang Z, Chen Y, Li H, Jiang C, et al. (2014) High-efficiency production of bioactive recombinant human fibroblast growth factor 18 in Escherichia coli and its effects on hair follicle growth. Appl Microbiol Biotechnol 98: 695-704. doi: 10.1007/s00253-013-4929-3 PMID: 23624709
22. Mohamed MR, Niles EG (2004) Transient and inducible expression of vaccinia/T7 recombinant viruses. Methods Mol Biol 269: 41-50. PMID: 15114006
23. Sakiyama H, Kaji K, Nakagawa K, Nagino K (1998) Inhibition of bFGF activity by complement C1s: covalent binding of C1s with bFGF. Cell Biochem Funct 16: 159-163. PMID: 9747507
24. Chen XJ, Sun FY, Xie QL, Liao MD, Zhang L, et al. (2002) Cloning and high level nonfusion expression of recombinant human basic fibroblast growth factor in Escherichia coli. Acta Pharmacol Sin 23: 782-786. PMID: 12230944
25. Efthymiadis A, Briggs LJ, Jans DA (1998) The HIV-1 Tat nuclear localization sequence confers novel nuclear import properties. J Biol Chem 273: 1623-1628. PMID: 9430704
26. Vaysse L, Gregory LG, Harbottle RP, Perouzel E, Tolmachov O, et al. (2006) Nuclear-targeted minicircle to enhance gene transfer with non-viral vectors in vitro and in vivo. J Gene Med 8: 754-763. PMID: 16532508
27. Tessler S, Neufeld G (1990) Basic fibroblast growth factor accumulates in the nuclei of various bFGF-producing cell types. J Cell Physiol 145: 310-317. PMID: 2246329
28. Manosroi J, Lohcharoenkal W, Gotz F, Werner RG, Manosroi W, et al. (2011) Transdermal absorption enhancement of N-terminal Tat-GFP fusion protein (TG) loaded in novel low-toxic elastic anionic niosomes. J Pharm Sci 100: 1525-1534. doi: 10.1002/jps.22355 PMID: 20891012
29. Wang Y, Su W, Li Q, Li C, Wang H, et al. (2013) Preparation and evaluation of lidocaine hydrochloride-loaded TAT-conjugated polymeric liposomes for transdermal delivery. Int J Pharm 441: 748-756. doi: 10.1016/j.ijpharm.2012.10.019 PMID: 23089577
30. Bloemen MC, van der Veer WM, Ulrich MM, van Zuijlen PP, Niessen FB, et al. (2009) Prevention and curative management of hypertrophic scar formation. Burns 35: 463-475. doi: 10.1016/j.burns.2008.07.016 PMID: 18951704
31. Jin R, Huang X, Li H, Yuan Y, Li B, et al. (2013) Laser therapy for prevention and treatment of pathologic excessive scars. Plast Reconstr Surg 132: 1747-1758. doi: 10.1097/PRS.0b013e3182a97e43 PMID: 24281600
32. van der Veer WM, Ferreira JA, de Jong EH, Molema G, Niessen FB (2010) Perioperative conditions affect long-term hypertrophic scar formation. Ann Plast Surg 65: 321-325. doi: 10.1097/SAP.0b013e3181c60f88 PMID: 20733369
33. Ward RS (1991) Pressure therapy for the control of hypertrophic scar formation after burn injury. A history and review. J Burn Care Rehabil 12: 257-262. PMID: 1885644
34. Shi HX, Lin C, Lin BB, Wang ZG, Zhang HY, et al. (2013) The anti-scar effects of basic fibroblast growth factor on the wound repair in vitro and in vivo. PLoS One 8: 2.
35. Verhaegen PD, Marle JV, Kuehne A, Schouten HJ, Gaffney EA, et al. (2012) Collagen bundle morphometry in skin and scar tissue: a novel distance mapping method provides superior measurements compared to Fourier analysis. J Microsc 245: 82-89. doi: 10.1111/j.1365-2818.2011.03547.x PMID: 21919907
36. Verhaegen PD, Schouten HJ, Tigchelaar-Gutter W, van Marle J, van Noorden CJ, et al. (2012) Adaptation of the dermal collagen structure of human skin and scar tissue in response to stretch: an experimental study. Wound Repair Regen 20: 658-666. doi: 10.1111/j.1524-475X.2012.00827.x PMID: 22882499