Biochemical characterization and structural analysis of a bifunctional cellulase/xylanase from Clostridium thermocellum

Journal of Biological Chemistry

Volumn 290, Issue 9, 2015, Pages 5739-5748

  Yuan, Shuo Fu ^b   Wu, Tzu Hui ^b   Lee, Hsiao Lin ^a   Hsieh, Han Yu ^b   Lin, Wen Ling ^a   Yang, Barbara ^b   Chang, Chih Kang ^a   Li, Qian ^c   Gao, Jian ^c   Huang, Chun Hsiang ^c   Ho, Meng Chiao ^a,b   Guo, Rey Ting ^c   Liang, Po Huang ^a,b  

^a INSTITUTE OF BIOLOGICAL CHEMISTRY   (Taiwan)

^b NATIONAL TAIWAN UNIVERSITY   (Taiwan)

^c Tianjin Airport Economic Area   (China)

Author keywords

[No Author keywords available]

Indexed keywords

ENZYME ACTIVITY; HYDROGEN BONDS;

BI-FUNCTIONAL; BIOCHEMICAL CHARACTERIZATION; CLOSTRIDIUM THERMOCELLUM; LOOP REGIONS; MANNANASE; SITE DIRECTED MUTAGENESIS; THERMOTOGA MARITIMA; XYLANASE ACTIVITY;

CLOSTRIDIUM;

ASPARAGINE; BETA MANNOSIDASE; CELLULASE; HISTIDINE; TRYPTOPHAN; XYLAN ENDO 1,3 BETA XYLOSIDASE; AMINO ACID; BACTERIAL PROTEIN; CELLOBIOSE; DISACCHARIDE; ENDO 1,4 BETA XYLANASE; PROTEIN BINDING; RECOMBINANT PROTEIN; XYLOBIOSE;

ARTICLE; BIOCHEMISTRY; CLOSTRIDIUM THERMOCELLUM; CONTROLLED STUDY; CRYSTAL STRUCTURE; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME SPECIFICITY; ENZYME STRUCTURE; ENZYME SUBSTRATE; HYDROGEN BOND; HYDROPHOBICITY; NONHUMAN; PH; PRIORITY JOURNAL; SEQUENCE HOMOLOGY; SITE DIRECTED MUTAGENESIS; STRUCTURE ANALYSIS; TEMPERATURE; THERMOTOGA MARITIMA; X RAY CRYSTALLOGRAPHY; AMINO ACID SEQUENCE; BINDING SITE; CHEMICAL STRUCTURE; CHEMISTRY; ENZYME ASSAY; ENZYMOLOGY; GENETICS; KINETICS; METABOLISM; MOLECULAR GENETICS; PROTEIN TERTIARY STRUCTURE;

CLOSTRIDIUM THERMOCELLUM; THERMOTOGA MARITIMA;

AMINO ACID SEQUENCE; AMINO ACIDS; BACTERIAL PROTEINS; BINDING SITES; CATALYTIC DOMAIN; CELLOBIOSE; CELLULASE; CLOSTRIDIUM THERMOCELLUM; CRYSTALLOGRAPHY, X-RAY; DISACCHARIDES; ENDO-1,4-BETA XYLANASES; ENZYME ASSAYS; KINETICS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; SUBSTRATE SPECIFICITY; THERMOTOGA MARITIMA;

EID: 84923776759     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M114.604454     Document Type: Article

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