Site-specific protein modification using immobilized sortase in batch and continuous-flow systems

Nature Protocols

Volumn 10, Issue 3, 2015, Pages 508-516

  Witte, Martin D ^a,d   Wu, Tongfei ^a,b   Guimaraes, Carla P ^a   Theile, Christopher S ^a   Blom, Annet E M ^a   Ingram, Jessica R ^a   Li, Zeyang ^a   Kundrat, Lenka ^a   Goldberg, Shalom D ^b   Ploegh, Hidde L ^a,c  

^a WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH   (United States)

^b JANSSEN RESEARCH AND DEVELOPMENT   (Belgium)

^c MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

^d UNIVERSITY OF GRONINGEN   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

LIPID; SEPHAROSE; SORTASE; SORTASE A; UNCLASSIFIED DRUG; AMINOACYLTRANSFERASE; BACTERIAL PROTEIN; CYSTEINE PROTEINASE; IMMOBILIZED ENZYME; PEPTIDYLTRANSFERASE; PROTEIN;

AFFINITY CHROMATOGRAPHY; AMINO TERMINAL SEQUENCE; ARTICLE; BATCH REACTOR; CARBOXY TERMINAL SEQUENCE; CATALYSIS; CONTINUOUS FLOW REACTOR; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME IMMOBILIZATION; ENZYME MECHANISM; ENZYME SUBSTRATE; GEL PERMEATION CHROMATOGRAPHY; LOW TEMPERATURE; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROCESS OPTIMIZATION; PROTEIN MODIFICATION; PROTEIN PROCESSING; PROTEIN PURIFICATION; PROTEIN SYNTHESIS; STAPHYLOCOCCUS AUREUS; SUPERNATANT; GENETICS; METABOLISM; MUTATION; PROCEDURES; PROTEIN ENGINEERING;

AMINOACYLTRANSFERASES; BACTERIAL PROTEINS; CATALYSIS; CYSTEINE ENDOPEPTIDASES; ENZYMES, IMMOBILIZED; MUTATION; PEPTIDYL TRANSFERASES; PROTEIN ENGINEERING; PROTEINS; SEPHAROSE;

EID: 84923763343     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2015.026     Document Type: Article

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