Assessment of erythroid dysplasia by "Difference from Normal" in routine clinical flow cytometry workup

Cytometry Part B - Clinical Cytometry

Volumn 88, Issue 2, 2015, Pages 125-135

  Brodersen, Lisa Eidenschink ^a   Menssen, Andrew J ^a   Wangen, Jamie R ^a   Stephenson, Christine F ^a   De Baca, Monica E ^a   Zehentner, Barbara K ^a   Wells, Denise A ^a   Loken, Michael R ^a  

^a HematoLogics ^*  (United States)

Author keywords

Ammonium chloride lysis; Dyserythropoiesis; Erythroid dysplasia; Erythroleukemia; Flow cytometry; Myelodysplastic syndrome; TP53

Indexed keywords

AMMONIUM CHLORIDE; CD36 ANTIGEN; CD71 ANTIGEN; CYANOCOBALAMIN; ENDOGLIN; FICOLL; FOLIC ACID; MEMBRANE ANTIGEN; MONOCLONAL ANTIBODY; PROTEIN P53;

ACUTE GRANULOCYTIC LEUKEMIA; AGED; ANTIGEN EXPRESSION; ARTICLE; BONE MARROW BIOPSY; CELL LINEAGE; COHORT ANALYSIS; CONTROLLED STUDY; CYANOCOBALAMIN DEFICIENCY; CYTOGENETICS; DYSERYTHROPOIESIS; DYSPLASIA; ERYTHROID CELL; ERYTHROID DYSPLASIA; ERYTHROID PRECURSOR CELL; ERYTHROLEUKEMIA; ERYTHROPOIESIS; FEMALE; FLOW CYTOMETRY; FOLIC ACID DEFICIENCY; HUMAN; HUMAN CELL; MAJOR CLINICAL STUDY; MALE; MYELODYSPLASTIC SYNDROME; NUTRITIONAL DEFICIENCY; PHENOTYPIC VARIATION; PRIORITY JOURNAL; RETICULOCYTE; FOLLOW UP; PATHOLOGY; PROCEDURES; STANDARDS; VERY ELDERLY;

AGED; AGED, 80 AND OVER; COHORT STUDIES; ERYTHROID CELLS; FEMALE; FLOW CYTOMETRY; FOLLOW-UP STUDIES; HUMANS; MALE; MYELODYSPLASTIC SYNDROMES;

EID: 84923642441     PISSN: 15524949     EISSN: 15524957     Source Type: Journal    
DOI: 10.1002/cyto.b.21199     Document Type: Article

References (35)

1. Wells DA, Benesch M, Loken MR, Vallego C, Myerson D, Leisenring WM, Deeg HJ. Myeloid and monocytic dyspoiesis as determined by flow cytometric scoring in myelodysplastic syndrome correlates with the IPSS and with outcome after hematopoietic stem cell transplantation. Blood. 2003;102:394-403.
2. Della Porta MG, Picone C, Pascutto C, et al. Multicentre validation of a reproducible flow cytometric score for the diagnosis of lowrisk myelodysplastic syndromes: Results of a European Leukemia-NET study. Haematologica 2012;8:1209-1217.
3. van de Loosdrecht AA, Alhan C, Bene MC, et al. Standardization of flow cytometry in myelodysplastic syndromes: Report from the first European Leukemia Net working conference on flow cytometry in myelodysplastic syndromes. Haematologica 2009;94:1124-1134.
4. Westers TM, Ireland R, Kern W, et al. Standardization of flow cytometry in myelodysplastic syndromes: A report from an international consortium and the European Leukemia Net Working Group. Leukemia 2012;26:1730-1741.
5. Porwit A, van de Loosdrecht AA, Bettelheim P, et al. Revisiting guidelines for integration of flow cytometry results in the WHO classification of Myelodysplastic Sybdrines-Proposal from the International/European Leukemia Net Working Group for Flow Cytometry in MDS (IMDSFlow). Leukemia 2014;28:1793-1798.
6. Della Porta MG, Francesco L, Del Vecchio L. Flow cytometry immunophenotyping for the evaluation of bone marrow dysplasia. Cytometry Part B 2011;80B:201-211.
7. Kern W, Haferlach C, Schnittger S, Haferlach T. Clinical utility of multiparameter flow cytometry in the diagnosis of 1013 patients with suspected myelodysplastic syndrome: Correlation to cytomorphology, cytogenetics, and clinical data. Cancer 2010;116:4549-4563.
8. Ogata K, Nakamura K, Yokose N, et al. Clinical significance of phenotypic features of blasts in patients with myelodysplastic syndrome. Blood 2002;100:3887-3896.
9. van de Loosdrecht AA, Westers TM, Westra AH, Drager AM, van der Velden VH, Ossenkoppele GJ. Identification of distinct prognostic subgroups in low and intermediate-1-risk myelodysplastic syndromes by flow cytometry. Blood 2008;111:1067-1077.
10. Scott BL, Wells DA, Loken MR, Myerson D, Leisenring WM, Deeg HJ. Validation of a flow cytometric scoring system as a prognostic indicator for posttransplantation outcome in patients with myelodysplastic syndrome. Blood 2008;112:2681-2686.
11. Stachurski D, Smith BR, Pozdnyakova O, Andersen M, Xiao Z, Raza A, Woda BA, Wang SA. Flow cytometric analysis of myelomonocytic cells by a pattern recognition approach is sensitive and specific in diagnosing myelodysplastic syndrome and related marrow diseases: Emphasis on a global evaluation and recognition of diagnostic pitfalls. Leuk Res 2008;32:215-224.
12. Chu SC, Wang TF, Li CC, Kao RH, Li DK, Su YC, Wells DA, Loken MR. Flow cytometric scoring system as a diagnostic and prognostic tool in myelodysplastic syndromes. Leuk Res 2011;7:868-873.
13. Kern W, Haferlach C, Schnittger S, Haferlach T. Serial assessment of suspected myelodysplastic syndromes: Significance of flow cytometric findings validated by cytomorphology, cytogenetics, and molecular genetics. Haematologica 2013;2:201-207.
14. Loken MR, van de Loosdrecht A, Ogata K, Orfao A, Wells DA. Flow cytometry in myelodysplastic syndromes: Report from a working conference. Leuk Res 2008;32:5-17.
15. Valent P, Horny HP, Bennett JM, et al. Definitions and standards in the diagnosis and treatment of the myelodysplastic syndromes: Consensus statements and report from a working conference. Leuk Res 2007;31:727-736.
16. Loken MR, Shah VO, Dattilio KL, Civin CI. Flow cytometric analysis of human bone marrow: I. Normal erythroid development. Blood 1987;69:255-263.
17. Malcovati L, Della Porta MG, Lunghi M, et al. Flow cytometry evaluation of erythroid and myeloid dysplasia in patients with myelodysplastic syndrome. Leukemia 2005;19:776-783.
18. Stetler-Stevenson M, Arthur DC, Jabbour N, Xie XY, Molldrem J, Barrett AJ, Venzon D, Rick ME. Diagnostic utility of flow cytometric immunophenotyping in myelodysplastic syndrome. Blood 2001;98:979-987.
19. Della Porta MG, Malcovati L, Invernizzi R, et al. Flow cytometry evaluation of erythroid dysplasia in patients with myelodysplastic syndrome. Leukemia 2006;20:549-555.
20. Xu F, Wu L, He Q, Zhang Z, Chang C, Li X. Immunophenotypic analysis of erythroid dysplasia and its diagnostic application in myelodysplastic syndromes. Intern Med J 2012;42:401-411.
21. Mathis S, Chapuis N, Debord C, et al. Flow cytometric detection of dyserythropoiesis: A sensitive and powerful diagnostic tool for myelodysplastic syndromes. Leukemia 2013;27:1981-1987.
22. Matarraz S, Lopez A, Barrena S, et al. Bone marrow cells from myelodysplastic syndromes show altered immunophenotypic profiles that may contribute to the diagnosis and prognostic stratification of the disease: A pilot study on a series of 56 patients. Cytometry Part B 2010;78:154-168.
23. Lorand-Metze I, Ribeiro E, Lima CS, Batista LS, Metze K. Detection of hematopoietic maturation abnormalities by flow cytometry in myelodysplastic syndromes and its utility for the differential diagnosis with non-clonal disorders. Leuk Res 2007;31:147-155.
24. Wangen JR, Eidenschink Brodersen L, Stolk TT, Wells DA, Loken MR. Assessment of normal erythropoiesis by flow cytometry: Important considerations for specimen preparation. Int J Lab Hematol 2014;36:184-196.
25. Fajtova M, Kovarikova A, Sevec P, Kankuri E, Sedlak J. Immunophenotypic profile of nucleated erythroid progenitors during maturation in regenerating bone marrow. Leuk Lymphoma 2013;11:2523-2530.
26. Matarraz S, Fernandez C, Albors M, Tredosio C, Lopez A, Jara-Acevedo M, Cervero C, Caballero G, Gutierrez O, Orfao A. Cellcycle distribution of different cell compartments in normal versus reactive bone marrow: A frame of reference for the study of dysplastic hematopoiesis. Cytometry Part B 2011;80B:354-361.
27. Machherndl-Spandl S, Suessner S, Danzer M, Proell J, Gabriel C, Lauf J, Sylie R, Klein HU, Béné MC, Weltermann A, Bettelheim P. Molecular pathways of early CD105-positive erythroid cells as compared with CD34-positive common precursor cells by flow cytometric cell-sorting and gene expression profiling. Blood Cancer J 2013;3: e100
28. Allan RW, Nsari-Lari MA, Jordan S. DRAQ5-based, no-lyse, no-wash bone marrow aspirate evaluation by flow cytometry. Am J Clin Pathol 2008;129:706-713.
29. Terstappen LW, Loken MR. Myeloid cell differentiation in normal bone marrow and acute myeloid leukemia assessed by multidimensional flow cytometry. Anal Cell Pathol 1990;2:229-240.
30. Owens M, Loken M. Flow cytometry principles for clinical laboratory practice: Quality assurance for quantitiative immunophenotyping. New York: Wiley-Liss; 1995.
31. Cutler JA, Wells DA, van de Loosdrecht AA, de Baca ME, Kalnoski MH, Zehentner BK, Eidenschink L, Ghiradelli KM, Biggerstaff JS, Loken MR. Phenotypic abnormalities strongly reflect genotype in patients with unexplained cytopenias. Cytometry Part B 2011;B3:150-157.
32. Swerdlow SH, Campo E, Harris NL, Jaffe ES, Pileri SA, Stein H, Thiele J, Vardiman JW, editors. WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues, 4th ed. Lyon, France: International Agency For Research on Cancer (IARC) Press; 2008.
33. Kuiper-Kramer PA, Huisman CM, Van der Molen-Sinke J, Abbes A, Van Eijk HG. The expression of transferrin receptors on erythroblasts in anaemia of chronic disease, myelodysplastic syndromes and iron deficiency. Acta Haematol 1997;97:127-131.
34. Chernyshev AV, Tarasov PA, Semianov KA, Nekrasov VM, Hoekstra AG, Maltsev VP. Erythrocyte lysis in isotonic solution of ammonium chloride: Theoretical modeling and experimental verification. J Theor Biol 2008;251:93-107.
35. Jacobs MH, Stewart DR. The role of carbonic anhydrase in certain ionic exchanges involving the erythrocyte. J Gen Physiol 1942;25: 539-552.