In-vivo RGB marking and multicolour single-cell tracking in the adult brain

Scientific Reports

Volumn 4, Issue , 2014, Pages

  Gomez Nicola, Diego ^a   Riecken, Kristoffer ^b   Fehse, Boris ^b   Perry, V Hugh ^a  

^a UNIVERSITY OF SOUTHAMPTON   (United Kingdom)

^b UNIVERSITY MEDICAL CENTER HAMBURG EPPENDORF   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

3T3 CELL LINE; ANIMAL; BRAIN; CYTOLOGY; FEMALE; GAMMARETROVIRUS; GENE VECTOR; HEK293 CELL LINE; HUMAN; LENTIVIRINAE; MALE; METABOLISM; MOLECULAR IMAGING; MOUSE; NERVE CELL;

ANIMALS; BRAIN; FEMALE; GAMMARETROVIRUS; GENETIC VECTORS; HEK293 CELLS; HUMANS; LENTIVIRUS; MALE; MICE; MOLECULAR IMAGING; NEURONS; NIH 3T3 CELLS;

EID: 84923304901     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep07520     Document Type: Article

References (44)

1. Livet, J. et al. Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous system. Nature 450, 56-62, doi:10.1038/nature06293 (2007).
2. Lichtman, J. W., Livet, J. & Sanes, J. R. A technicolour approach to the connectome. Nat Rev Neurosci 9, 417-422, doi:10.1038/nrn2391 (2008).
3. Feng, G. et al. Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP. Neuron 28, 41-51 (2000).
4. Zong, H., Espinosa, J. S., Su, H. H., Muzumdar, M. D. & Luo, L. Mosaic analysis with double markers in mice. Cell 121, 479-492, doi:10.1016/j.cell.2005.02.012 (2005).
5. Geraerts, M. et al. Lentiviral vectors mediate efficient and stable gene transfer in adult neural stem cells in vivo. Hum Gene Ther 17, 635-650, doi:10.1089/ hum.2006.17.635 (2006).
6. Zhao, C., Teng, E. M., Summers, R. G., Jr., Ming, G. L. & Gage, F. H. Distinct morphological stages of dentate granule neuron maturation in the adult mouse hippocampus. J Neurosci 26, 3-11, doi:10.1523/JNEUROSCI.3648-05.2006 (2006).
7. Cai, D., Cohen, K. B., Luo, T., Lichtman, J.W. & Sanes, J. R. Improved tools for the Brainbow toolbox. Nat Methods 10, 540-547 (2013).
8. Garcia-Marques, J. & Lopez-Mascaraque, L. Clonal identity determines astrocyte cortical heterogeneity. Cereb Cortex 23, 1463-1472, doi:10.1093/cercor/bhs134 (2013).
9. Loulier, K. et al. Multiplex cell and lineage tracking with combinatorial labels. Neuron 81, 505-520, doi:10.1016/j.neuron.2013.12.016 (2014).
10. Garcia-Moreno, F., Vasistha, N. A., Begbie, J. & Molnar, Z. CLoNe is a new method to target single progenitors and study their progeny in mouse and chick. Development 141, 1589-1598, doi:10.1242/dev.105254 (2014).
11. Weber, K. et al. RGB marking facilitates multicolor clonal cell tracking. Nat Med 17, 504-509, doi:10.1038/nm.2338 (2011).
12. Weber, K., Thomaschewski, M., Benten, D. & Fehse, B. RGB marking with lentiviral vectors for multicolor clonal cell tracking. Nat Protoc 7, 839-849, doi:10.1038/nprot.2012.026 (2012).
13. Weber, K., Mock, U., Petrowitz, B., Bartsch, U. & Fehse, B. Lentiviral gene ontology (LeGO) vectors equipped with novel drug-selectable fluorescent proteins: new building blocks for cell marking and multi-gene analysis. Gene Ther 17, 511-520, doi:10.1038/gt.2009.149 (2010).
14. Weber, K., Bartsch, U., Stocking, C. & Fehse, B. A multicolor panel of novel lentiviral "gene ontology" (LeGO) vectors for functional gene analysis. Mol Ther 16, 698-706, doi:10.1038/mt.2008.6 (2008).
15. Baum, C. et al. The potent enhancer activity of the polycythemic strain of spleen focus-forming virus in hematopoietic cells is governed by a binding site for Sp1 in the upstream control region and by a unique enhancer core motif, creating an exclusive target for PEBP/CBF. J Virol 71, 6323-6331 (1997).
16. Schambach, A. et al. Equal potency of gammaretroviral and lentiviral SIN vectors for expression of O6-methylguanine-DNA methyltransferase in hematopoietic cells. Mol Ther 13, 391-400, doi:10.1016/j.ymthe.2005.08.012 (2006).
17. Kuroda, H., Kutner, R. H., Bazan, N. G. & Reiser, J. A comparative analysis of constitutive and cell-specific promoters in the adult mouse hippocampus using lentivirus vector-mediated gene transfer. J Gene Med 10, 1163-1175, doi:10.1002/ jgm.1249 (2008).
18. Schambach, A. et al. Overcoming promoter competition in packaging cells improves production of self-inactivating retroviral vectors. Gene Ther 13, 1524-1533, doi:10.1038/sj.gt.3302807 (2006).
19. Naldini, L., Blomer, U., Gage, F. H., Trono, D. & Verma, I. M. Efficient transfer, integration, and sustained long-term expression of the transgene in adult rat brains injected with a lentiviral vector. Proc Natl Acad Sci U S A 93, 11382-11388 (1996).
20. van den Pol, A. N. & Ghosh, P. K. Selective neuronal expression of green fluorescent protein with cytomegalovirus promoter reveals entire neuronal arbor in transgenic mice. J Neurosci 18, 10640-10651 (1998).
21. Jefferis, G. S. & Livet, J. Sparse and combinatorial neuron labelling. Curr Opin Neurobiol 22, 101-110, doi:10.1016/j.conb.2011.09.010 (2012).
22. Boldogkoi, Z. et al. Genetically timed, activity-sensor and rainbow transsynaptic viral tools. Nat Methods 6, 127-130, doi:10.1038/nmeth.1292 (2009).
23. Deshpande, A. et al. Retrogrademonosynaptic tracing reveals the temporal evolution of inputs onto new neurons in the adult dentate gyrus and olfactory bulb. Proc Natl Acad Sci U S A 110, E1152-1161, doi:10.1073/pnas.1218991110 (2013).
24. Vivar, C. et al. Monosynaptic inputs to new neurons in the dentate gyrus. Nat Commun 3, 1107, doi:10.1038/ncomms2101 (2012).
25. Cayre, M., Canoll, P. & Goldman, J. E. Cell migration in the normal and pathological postnatal mammalian brain. Prog Neurobiol 88, 41-63, doi:10.1016/ j.pneurobio.2009.02.001 (2009).
26. Ming, G. L. & Song, H. Adult neurogenesis in the mammalian central nervous system. Annu Rev Neurosci 28, 223-250, doi:10.1146/ annurev.neuro.28.051804.101459 (2005).
27. Saito, T. & Nakatsuji, N. Efficient gene transfer into the embryonic mouse brain using in vivo electroporation. Dev Biol 240, 237-246, doi:10.1006/dbio.2001.0439 (2001).
28. Boutin, C.,Diestel, S., Desoeuvre, A., Tiveron,M. C.& Cremer, H. Efficient in vivo electroporation of the postnatal rodent forebrain. PLoS One 3, e1883, doi:10.1371/ journal.pone.0001883 (2008).
29. Gomez-Nicola, D. et al. Temporal dynamics of hippocampal neurogenesis in chronic neurodegeneration. Brain, 137, 2312-28. doi: 10.1093/brain/awu155. (2014).
30. Yu, Y. C., Bultje, R. S., Wang, X. & Shi, S. H. Specific synapses develop preferentially among sister excitatory neurons in the neocortex. Nature 458, 501-504, doi:10.1038/nature07722 (2009).
31. Coffey, S. E., Giedt, R. J.&Weissleder, R. Automated analysis of clonal cancer cells by intravital imaging. IntraVital 2, e26138 (2013).
32. Malide, D., Metais, J. Y. & Dunbar, C. E. Dynamic clonal analysis of murine hematopoietic stem and progenitor cells marked by 5 fluorescent proteins using confocal and multiphoton microscopy. Blood 120, e105-116, doi:10.1182/blood-2012-06-440636 (2012).
33. Cornils, K. et al. Multiplexing clonality: combining RGB marking and genetic barcoding. Nucleic Acids Res 42, e56, doi:10.1093/nar/gku081 (2014).
34. Rizzo, M. A., Springer, G. H., Granada, B. & Piston, D. W. An improved cyan fluorescent protein variant useful for FRET. Nat Biotechnol 22, 445-449, doi:10.1038/nbt945 (2004).
35. Shaner, N. C. et al. Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nat Biotechnol 22, 1567-1572, doi:10.1038/nbt1037 (2004).
36. Nagai, T. et al. A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat Biotechnol 20, 87-90, doi:10.1038/nbt0102-87 (2002).
37. Dull, T. et al. A third-generation lentivirus vector with a conditional packaging system. J Virol 72, 8463-8471 (1998).
38. Beyer, W. R., Westphal, M., Ostertag, W. & von Laer, D. Oncoretrovirus and lentivirus vectors pseudotyped with lymphocytic choriomeningitis virus glycoprotein: generation, concentration, and broad host range. J Virol 76, 1488-1495 (2002).
39. Morita, S., Kojima, T. & Kitamura, T. Plat-E: an efficient and stable system for transient packaging of retroviruses. Gene Ther 7, 1063-1066, doi:10.1038/ sj.gt.3301206 (2000).
40. Gomez-Nicola, D., Valle-Argos, B., Pallas-Bazarra, N. & Nieto-Sampedro, M. Interleukin-15 regulates proliferation and self-renewal of adult neural stem cells. Mol Biol Cell 22, 1960-1970, doi: 10.1091/mbc.E11-01-0053 (2011).
41. Gomez-Nicola, D., Fransen, N. L., Suzzi, S. & Perry, V. H. Regulation of microglial proliferation during chronic neurodegeneration. J Neurosci 33, 2481-2493, doi:10.1523/JNEUROSCI.4440-12.2013 (2013).
42. Gomez-Nicola, D., Schetters, S. T. & Hugh Perry, V. Differential role of CCR2 in the dynamics of microglia and perivascular macrophages during prion disease. Glia, 62, 1041-52. doi: 10.1002/glia.22660 (2014).
43. Sholl, D. A. Dendritic organization in the neurons of the visual and motor cortices of the cat. J Anat 87, 387-406 (1953).
44. Toni, N. et al. Neurons born in the adult dentate gyrus form functional synapses with target cells. Nat Neurosci 11, 901-907, doi:10.1038/nn.2156 (2008).