Concerning the role of cell lysis-cryptic growth in anaerobic side-stream reactors: The single-cell analysis of viable, dead and lysed bacteria

Water Research

Volumn 74, Issue , 2015, Pages 132-142

  Foladori, P ^a   Velho, V F ^b   Costa, R H R ^b   Bruni, L ^c   Quaranta, A ^a   Andreottola, G ^a  

^a UNIVERSITY OF TRENTO   (Italy)

^b FEDERAL UNIVERSITY OF SANTA CATARINA   (Brazil)

^c Agenzia per la Depurazione Autonomous Province of Trento   (Italy)

Author keywords

Anaerobic side stream reactor; Cell lysis; Cryptic growth; Flow cytometry; Sludge reduction

Indexed keywords

AEROBIC BACTERIA; CELLS; CYTOLOGY; DECAY (ORGANIC); ECOLOGY; FLOW CYTOMETRY; METABOLISM;

AEROBIC AND ANAEROBIC CONDITIONS; ANAEROBICS; ANAEROBIOSIS; ANEROBIC SIDE-STREAM REACTOR; BACTERIAL CELLS; CELL LYSIS; CRYPTIC GROWTHS; ENZYMATIC ACTIVITIES; SIDE STREAMS; SLUDGE REDUCTION;

HYDROLYSIS;

FLUORESCENT DYE; NITROGEN; ORGANIC MATTER; OXYGEN; SEWAGE;

BACTERIOLOGY; BACTERIUM; BIODEGRADATION; BIOREACTOR; CRYPSIS; ENZYME ACTIVITY; FLOW CYTOMETRY; HYDROLYSIS; LYSIS; OXIC CONDITIONS; SLUDGE; SOLUBILIZATION; VIABILITY;

ACTIVATED SLUDGE; AEROBIC METABOLISM; ANAEROBIC DIGESTION; ANAEROBIC GROWTH; ANAEROBIC REACTOR; ANAEROBIC SIDE STREAM REACTOR; ARTICLE; BACTERIAL CELL; BACTERIAL GROWTH; BACTERIAL VIABILITY; BACTERIOLYSIS; BIODEGRADABILITY; CELL VOLUME; CHEMICAL OXYGEN DEMAND; CONTROLLED STUDY; CYTOLYSIS; DRY WEIGHT; ENVIRONMENTAL TEMPERATURE; ENZYME ACTIVITY; FILTRATION; FLOW CYTOMETRY; FLUORESCENCE RESONANCE ENERGY TRANSFER; HYDROLYSIS; NITRIFICATION; NONHUMAN; OXIDATION; OXYGEN CONSUMPTION; PRIORITY JOURNAL; SINGLE CELL ANALYSIS; SOLUBILIZATION; SUSPENDED PARTICULATE MATTER; BACTERIUM; BIOCHEMICAL OXYGEN DEMAND; BIOREACTOR; CHEMISTRY; ENZYMOLOGY; GROWTH, DEVELOPMENT AND AGING; METABOLISM; MICROBIOLOGY; OXIDATION REDUCTION REACTION; PROCEDURES; SEWAGE;

BACTERIA (MICROORGANISMS);

AEROBIOSIS; ANAEROBIOSIS; BACTERIA; BIOLOGICAL OXYGEN DEMAND ANALYSIS; BIOREACTORS; NITROGEN; OXIDATION-REDUCTION; SEWAGE; SINGLE-CELL ANALYSIS; WASTE DISPOSAL, FLUID;

EID: 84923283137     PISSN: 00431354     EISSN: 18792448     Source Type: Journal    
DOI: 10.1016/j.watres.2015.01.042     Document Type: Article

References (30)

1. An K., Chen G. Chemical oxygen demand and the mechanism of excess sludge reduction in an oxic-settling-anaerobic activated sludge process. J. Environ. Eng. 2008, 134(6):469-477.
2. APHA, AWWA, WERF Standard Methods for the Examination of Water and Wastewater 2012, Washington, DC. 22nd ed.
3. Chon D.H., McNamara R., Kim H.S., Park C. Investigating the mechanism of sludge reduction in activated sludge with an anaerobic side-stream reactor. Water Sci. Technol. 2011, 63:93-99.
4. Chon D.H., Rome M., Kim Y.M., Park K.Y., Park C. Investigation of the sludge reduction mechanism in the anaerobic side-stream reactor process using several control biological wastewater treatment processes. Water Res. 2011, 45:6021-6029.
5. Chudoba P., Chudoba J., Capdeville B. The aspect of energetic uncoupling of microbial growth in the activated sludge process: OSA system. Water Sci. Technol. 1992, 26(9-11):2477-2480.
6. Chudoba B., Morel A., Capdeville B. The case of both energetic uncoupling and metabolic selection of microorganisms in the OSA activated sludge system. Environ. Technol. 1992, 13:761-770.
7. Coma M., Rovira S., Canals J., Colprim J. Minimization of sludge production by a side-stream reactor under anoxic conditions in a pilot plant. Bioresour. Technol. 2013, 129:229-235.
8. Foladori P., Bruni L., Andreottola G., Ziglio G. Effects of sonication on bacteria viability in wastewater treatment plants evaluated by flow cytometry - fecal indicators, wastewater and activated sludge. Water Res. 2007, 41:235-243.
9. Foladori P., Andreottola G., Ziglio G. Sludge Reduction Technologies in Wastewater Treatment Plants 2010, IWA Publishing, London, 2010.
10. Foladori P., Bruni L., Tamburini S., Ziglio G. Direct quantification of bacterial biomass in influent, effluent and activated sludge of wastewater treatment plants by using flow cytometry. Water Res. 2010, 44(13):3807-3818.
11. Fry J.C. Direct methods and biomass estimation. Methods Microbiol. 1990, 22:41-85.
12. Goel R., Noguera D. Evaluation of sludge yield and phosphorus removal in a Cannibal solids reduction process. J. Environ. Eng. 2006, 132:1331-1337.
13. Henze M., Gujer W., Mino T., Matsuo T., Wentzel M.C., Marais G.v.R., van Loosdrecht M.C.M. Activated sludge model No. 2D, ASM2D. Water Sci. Tech. 1999, 39(1):165-182.
14. Johnson B.R., Diagger G.T., Novak J.T. The use of ASM based models for the simulation of biological sludge reduction processes. Water Pract. Technol. 2008, 3(3). 10.2166/WPT.2008074.
15. Kim Y.M., Chon D.H., Kim H.S., Park C. Investigation of bacterial community in activated sludge with an anaerobic side-stream reactor (ASSR) to decrease the generation of excess sludge. Water Res. 2012, 46:4292-4300.
16. Li X., Liu X., Wu S., Rasool A., Zuo J., Li C., Liu G. Microbial diversity and community distribution in different functional zones of continuous aerobic-anaerobic coupled process for sludge in situ reduction. Chem. Eng. J. 2014, 257:74-81.
17. Munz G., Lubello C., Oleszkiewicz J.A. Modeling the decay of ammonium oxidizing bacteria. Water Res. 2011, 45:557-564.
18. Nebe-von-Caron G., Stephens P.J., Hewitt C.J., Powell J.R., Badley R.A. Analysis of bacterial function by multi-colour fluorescence flow cytometry and single cell sorting. J. Microbiol. Methods 2000, 42:97-114.
19. Novak J.T., Sadler M.E., Murthy S.N. Mechanisms of floc destruction during anaerobic and aerobic digestion and the effect on conditioning and dewatering of biosolids. Water Res. 2003, 37:3136-3144.
20. Novak J.T., Chon D.H., Curtis B., Doyle M. Biological solids reduction using Cannibal process. Water Environ. Res. 2007, 79(12):2380-2386.
21. Park C., Abu-Orf M.M., Novak J.T. The digestibility of waste activated sludges. Water Environ. Res. 2006, 78:59-68.
22. Quan F., Anfeng Y., Libing C., Hongzhang C., Xing X.-H. Mechanistic study of on-site sludge reduction in a baffled bioreactor consisting of three series of alternating aerobic and anaerobic compartments. Biochem. Eng. J. 2012, 67:45-51.
23. Semblante G.U., Hai F.I., Ngo H.H., Guo W., You S.-J., Price W.E., Nghiem L.D. Sludge cycling between aerobic, anoxic and anaerobic regimes to reduce sludge production during wastewater treatment: performance, mechanisms, and implications. Bioresour. Technol. 2014, 155:395-409.
24. Steen H.B. Flow cytometry of bacteria: glimpses from the past with a view to the future. J. Microbiol. Methods 2000, 42:65-74.
25. Tracy B.P., Gaida S.M., Papoutsakis E.T. Flow cytometry for bacteria: enabling metabolic engineering, synthetic biology and the elucidation of complex phenotypes. Curr. Opin. Biotechnol. 2010, 21:85-99.
26. Troiani C., Eusebi A.L., Battistoni P. Excess sludge reduction by biological way: from experimental experience to a real full scale application. Bioresour. Technol. 2011, 102:10352-10358.
27. Wang J., Zhao Q., Jin W., Lin J. Mechanism on minimization of excess sludge in oxic-settling-anaerobic (OSA) process. China. Front. Environ. Sci. Eng. 2008, 36-43.
28. Wei Y., Van Houten R.T., Borger A.R., Eikelboom D.H., Fan Y. Minimization of excess sludge production for biological wastewater treatment. Water Res. 2003, 37:4453-4467.
29. Zhou Z., Qiao W., Xing C., An Y., Shen X., Ren W., Jiang L., Wang L. Microbial community structure of anoxic-oxic-settling-anaerobic sludge reduction process revealed by 454-pyrosequencing. Chem. Eng. J. 2015, 266:249-257.
30. Ziglio G., Andreottola G., Barbesti S., Boschetti G., Bruni L., Foladori P., Villa R. Assessment of activated sludge viability with flow cytometry. Water Res. 2002, 36:460-468.