Co-expression of two polyhydroxyalkanoate synthase subunits from synechocystis sp. PCC 6803 by cell-free synthesis and their specific activity for polymerization of 3-hydroxybutyryl-coenzyme A

Biochemistry

Volumn 54, Issue 6, 2015, Pages 1401-1407

  Numata, Keiji ^a   Motoda, Yoko ^a   Watanabe, Satoru ^b   Osanai, Takashi ^a   Kigawa, Takanori ^b  

^a Elements Chemistry Laboratory   (Japan)

^b RIKEN   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

CLONING; ORGANIC POLYMERS;

BIOCHEMICAL PROPERTIES; CELL FREE SYNTHESIS; HILL COEFFICIENT; METABOLIC REACTIONS; POLYHYDROXYALKANOATE SYNTHASE; POLYHYDROXYALKANOATES; SPECIFIC ACTIVITY; SYNECHOCYSTIS SP. PCC 6803;

SIZE EXCLUSION CHROMATOGRAPHY;

3 HYDROXYBUTYRYL COENZYME A; ACYLTRANSFERASE; COENZYME A; POLYHYDROXYALKANOATE SYNTHASE; POLYHYDROXYALKANOATE SYNTHASE C; POLYHYDROXYALKANOATE SYNTHASE CE; UNCLASSIFIED DRUG; POLY(3-HYDROXYALKANOIC ACID) SYNTHASE;

ANIMAL CELL; ARTICLE; BACTERIAL STRAIN; CELL FREE SYNTHESIS SYSTEM; CELL FREE SYSTEM; CONTROLLED STUDY; CYANOBACTERIUM; ENZYME ACTIVITY; ENZYME METABOLISM; ENZYME SPECIFICITY; ENZYME SYNTHESIS; GEL PERMEATION CHROMATOGRAPHY; HILL COEFFICIENT; NONHUMAN; POLYMERIZATION; PRIORITY JOURNAL; PROTEIN EXPRESSION; SYNECHOCYSTIS; ENZYMOLOGY; GENETICS; METABOLISM; MOLECULAR CLONING; POLYACRYLAMIDE GEL ELECTROPHORESIS;

CYANOBACTERIA; SYNECHOCYSTIS SP. PCC 6803;

ACYLTRANSFERASES; CELL-FREE SYSTEM; CLONING, MOLECULAR; ELECTROPHORESIS, POLYACRYLAMIDE GEL; POLYMERIZATION; SYNECHOCYSTIS;

EID: 84923171797     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/bi501560b     Document Type: Article

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