Experimental validation of plant peroxisomal targeting prediction algorithms by systematic comparison of in vivo import efficiency and in vitro PTS1 binding affinity

Journal of Molecular Biology

Volumn 427, Issue 5, 2015, Pages 1085-1101

  Skoulding, Nicola S ^a   Chowdhary, Gopal ^b,c   Deus, Mara J ^b   Baker, Alison ^a   Reumann, Sigrun ^b,d   Warriner, Stuart L ^a  

^a UNIVERSITY OF LEEDS   (United Kingdom)

^b UNIVERSITY OF STAVANGER   (Norway)

^c KIIT UNIVERSITY   (India)

^d UNIVERSITY OF HAMBURG   (Germany)

Author keywords

fluorescence anisotropy; peptide; PEX5; specificity; YFP fusion

Indexed keywords

AMINO ACID; C TERMINAL TARGETING SIGNAL TYPE 1; MATRIX PROTEIN; PEX5 RECEPTOR; RECEPTOR; TRIPEPTIDE; UNCLASSIFIED DRUG; VEGETABLE PROTEIN; PROTEIN BINDING; SIGNAL PEPTIDE;

ALGORITHM; ARABIDOPSIS; ARTICLE; BINDING AFFINITY; BINDING ASSAY; COMPARATIVE STUDY; COMPUTER MODEL; CONTROLLED STUDY; ENHANCER REGION; EPIDERMIS CELL; ESCHERICHIA COLI; GENOME SIZE; IN VITRO STUDY; IN VIVO STUDY; NONHUMAN; ONION; PEROXISOMAL TARGETING SIGNAL; PEROXISOME; PICHIA; POINT MUTATION; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN SECONDARY STRUCTURE; THERMODYNAMICS; TRANSFORMED CELL; VASCULAR PLANT; AMINO ACID SEQUENCE; METABOLISM; PHYSIOLOGY; PROTEIN TERTIARY STRUCTURE; PROTEIN TRANSPORT;

EMBRYOPHYTA;

ALGORITHMS; AMINO ACID SEQUENCE; AMINO ACIDS; PEROXISOMES; PLANT PROTEINS; PROTEIN BINDING; PROTEIN SORTING SIGNALS; PROTEIN STRUCTURE, TERTIARY; PROTEIN TRANSPORT;

EID: 84923111325     PISSN: 00222836     EISSN: 10898638     Source Type: Journal    
DOI: 10.1016/j.jmb.2014.12.003     Document Type: Article

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