Osteopontin binding to the alpha 4 integrin requires highest affinity integrin conformation, but is independent of post-translational modifications of osteopontin

Matrix Biology

Volumn 41, Issue , 2015, Pages 19-25

  Hui, Tommy ^a   Sørensen, Esben S ^b   Rittling, Susan R ^a,c  

^a FORSYTH INSTITUTE   (United States)

^b AARHUS UNIVERSITY   (Denmark)

^c HARVARD SCHOOL OF DENTAL MEDICINE   (United States)

Author keywords

Adhesion; Integrin Alpha4; Osteopontin; Phosphorylation; VCAM; VLA 4

Indexed keywords

ALPHA4 INTEGRIN; ALPHA5 INTEGRIN; ALPHA9 INTEGRIN; CALCIUM ION; FIBRONECTIN; INTEGRIN; MAGNESIUM ION; OSTEOPONTIN; PEPTIDE; THROMBIN; UNCLASSIFIED DRUG; VASCULAR CELL ADHESION MOLECULE 1; SPP1 PROTEIN, HUMAN; VERY LATE ACTIVATION ANTIGEN 5;

ARTICLE; BINDING SITE; CARBOXY TERMINAL SEQUENCE; CELL ADHESION; GLYCOSYLATION; HUMAN; HUMAN CELL; IN VITRO STUDY; JURKAT CELL LINE; LEUKOCYTE; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN CLEAVAGE; PROTEIN CONFORMATION; PROTEIN PHOSPHORYLATION; CHEMISTRY; COMPARATIVE STUDY; CYTOLOGY; METABOLISM; PHOSPHORYLATION; PROTEIN PROCESSING; TUMOR CELL LINE;

BINDING SITES; CELL LINE, TUMOR; HUMANS; INTEGRIN ALPHA4; INTEGRIN ALPHA5BETA1; JURKAT CELLS; LEUKOCYTES; OSTEOPONTIN; PHOSPHORYLATION; PROTEIN CONFORMATION; PROTEIN PROCESSING, POST-TRANSLATIONAL; THROMBIN; VASCULAR CELL ADHESION MOLECULE-1;

EID: 84922735977     PISSN: 0945053X     EISSN: 15691802     Source Type: Journal    
DOI: 10.1016/j.matbio.2014.11.005     Document Type: Article

References (30)

1. Ausubel F.B., R, Kingston R.E., Moore D.D., Seidman J.G., Smith J.A., Struhl K. Short protocols in molecular biology 1995, John Wiley & Sons, New York.
2. Barry S.T., Ludbrook S.B., Murrison E., Horgan C.M. Analysis of the alpha4beta1 integrin-osteopontin interaction. Exp. Cell Res. 2000, 258:342-351.
3. Barry S.T., Ludbrook S.B., Murrison E., Horgan C.M. A regulated interaction between alpha5beta1 integrin and osteopontin. Biochem. Biophys. Res. Commun. 2000, 267:764-769.
4. Bayless K.J., Meininger G.A., Scholtz J.M., Davis G.E. Osteopontin is a ligand for the α4β1 integrin. J. Cell Sci. 1998, 111:1165-1174.
5. Chigaev A., Sklar L.A. Aspects of VLA-4 and LFA-1 regulation that may contribute to rolling and firm adhesion. Front. Immunol. 2012, 3:242.
6. Chigaev A., Blenc A.M., Braaten J.V., Kumaraswamy N., Kepley C.L., Andrews R.P., Oliver J.M., Edwards B.S., Prossnitz E.R., Larson R.S., Sklar L.A. Real time analysis of the affinity regulation of alpha 4-integrin. The physiologically activated receptor is intermediate in affinity between resting and Mn(2+) or antibody activation. J. Biol. Chem. 2001, 276:48670-48678.
7. Chigaev A., Buranda T., Dwyer D.C., Prossnitz E.R., Sklar L.A. FRET detection of cellular alpha4-integrin conformational activation. Biophys. J. 2003, 85:3951-3962.
8. Chigaev A., Waller A., Amit O., Halip L., Bologa C.G., Sklar L.A. Real-time analysis of conformation-sensitive antibody binding provides new insights into integrin conformational regulation. J. Biol. Chem. 2009, 284:14337-14346.
9. Christensen B., Nielsen M.S., Haselmann K.F., Petersen T.E., Sorensen E.S. Post-translationally modified residues of native human osteopontin are located in clusters: identification of 36 phosphorylation and five O-glycosylation sites and their biological implications. Biochem. J. 2005, 390:285-292.
10. Christensen B., Kazanecki C.C., Petersen T.E., Rittling S.R., Denhardt D.T., Sorensen E.S. Cell type-specific post-translational modifications of mouse osteopontin are associated with different adhesive properties. J. Biol. Chem. 2007, 282:19463-19472.
11. Christensen B., Schack L., Klaning E., Sorensen E.S. Osteopontin is cleaved at multiple sites close to its integrin-binding motifs in milk and is a novel substrate for plasmin and cathepsin D. J. Biol. Chem. 2010, 285:7929-7937.
12. Christensen B., Klaning E., Nielsen M.S., Andersen M.H., Sorensen E.S. C-terminal modification of osteopontin inhibits interaction with the alphaVbeta3-integrin. J. Biol. Chem. 2012, 287:3788-3797.
13. Engelhardt B., Briskin M.J. Therapeutic targeting of alpha 4-integrins in chronic inflammatory diseases: tipping the scales of risk towards benefit?. Eur. J. Immunol. 2005, 35:2268-2273.
14. Grassinger J., Haylock D.N., Storan M.J., Haines G.O., Williams B., Whitty G.A., Vinson A.R., Be C.L., Li S., Sorensen E.S., Tam P.P., Denhardt D.T., Sheppard D., Choong P.F., Nilsson S.K. Thrombin-cleaved osteopontin regulates hemopoietic stem and progenitor cell functions through interactions with alpha9beta1 and alpha4beta1 integrins. Blood 2009, 114:49-59.
15. Green P.M., Ludbrook S.B., Miller D.D., Horgan C.M., Barry S.T. Structural elements of the osteopontin SVVYGLR motif important for the interaction with alpha(4) integrins. FEBS Lett. 2001, 503:75-79.
16. Hu D.D., Hoyer J.R., Smith J.W. Calcium suppresses cell adhesion to osteopontin by attenuating binding affinity for integrin αvβ3 J. Biol. Chem. 1995, 270:9917-9925.
17. Hu D.D., Lin E.C.K., Kovach N.L., Hoyer J.R., Smith J.W. A biochemical characterization of the binding of osteopontin to integrins αvβ1 and αvβ5 J. Biol. Chem. 1995, 270:26232-26238.
18. Ito K., Kon S., Nakayama Y., Kurotaki D., Saito Y., Kanayama M., Kimura C., Diao H., Morimoto J., Matsui Y., Uede T. The differential amino acid requirement within osteopontin in [alpha]4 and [alpha]9 integrin-mediated cell binding and migration. Matrix Biol. 2009, 28:11-19.
19. Katoh K., Mohri H., Ogawa K., Okubo T. Human plasma fibronectin mediates adhesion of U937 Cells by RGD and CS1. J. Thromb. Thrombolysis 1998, 5:125-134.
20. Lund S.A., Wilson C.L., Raines E.W., Tang J., Giachelli C.M., Scatena M. Osteopontin mediates macrophage chemotaxis via alpha4 and alpha9 integrins and survival via the alpha4 integrin. J. Cell. Biochem. 2013, 114:1194-1202.
21. McKee M.D., Nanci A. Osteopontin at mineralized tissue interfaces in bone, teeth, and osseointegrated implants: ultrastructural distribution and implications for mineralized tissue formation, turnover, and repair. Microsc. Res. Tech. 1996, 33:141-164.
22. Myles T., Nishimura T., Yun T.H., Nagashima M., Morser J., Patterson A.J., Pearl R.G., Leung L.L. Thrombin activatable fibrinolysis inhibitor, a potential regulator of vascular inflammation. J. Biol. Chem. 2003, 278:51059-51067.
23. Pepinsky R.B., Mumford R.A., Chen L.L., Leone D., Amo S.E., Riper G.V., Whitty A., Dolinski B., Lobb R.R., Dean D.C., Chang L.L., Raab C.E., Si Q., Hagmann W.K., Lingham R.B. Comparative assessment of the ligand and metal ion binding properties of integrins alpha9beta1 and alpha4beta1. Biochemistry 2002, 41:7125-7141.
24. Rittling S.R., Chen Y., Feng F., Wu Y. Tumor-derived osteopontin is soluble, not matrix associated. J. Biol. Chem. 2002, 277:9175-9182.
25. Rose D.M., Han J., Ginsberg M.H. Alpha4 integrins and the immune response. Immunol. Rev. 2002, 186:118-124.
26. Sanz-Rodriguez F., Hidalgo A., Teixido J. Chemokine stromal cell-derived factor-1alpha modulates VLA-4 integrin-mediated multiple myeloma cell adhesion to CS-1/fibronectin and VCAM-1. Blood 2001, 97:346-351.
27. Schneider U., Schwenk H.U., Bornkamm G. Characterization of EBV-genome negative "null" and "T" cell lines derived from children with acute lymphoblastic leukemia and leukemic transformed non-Hodgkin lymphoma. Int. J. Cancer 1977, 19:621-626.
28. Steinman L. Shifting therapeutic attention in MS to osteopontin, type 1 and type 2 IFN. Eur. J. Immunol. 2009, 39:2358-2360.
29. Vanderslice P., Woodside D.G., Caivano A.R., Decker E.R., Munsch C.L., Sherwood S.J., Lejeune W.S., Miyamoto Y.J., McIntyre B.W., Tilton R.G., Dixon R.A. Potent in vivo suppression of inflammation by selectively targeting the high affinity conformation of integrin alpha4beta1. Biochem. Biophys. Res. Commun. 2010, 400:619-624.
30. Yednock T.A., Cannon C., Vandevert C., Goldbach E.G., Shaw G., Ellis D.K., Liaw C., Fritz L.C., Tanner L.I. Alpha 4 beta 1 integrin-dependent cell adhesion is regulated by a low affinity receptor pool that is conformationally responsive to ligand. J. Biol. Chem. 1995, 270:28740-28750.