Establishment of a sensitive system for analysis of human vaginal microbiota on the basis of rRNA-targeted reverse transcription-quantitative PCR

Journal of Microbiological Methods

Volumn 111, Issue , 2015, Pages 93-104

  Kurakawa, Takashi ^a   Ogata, Kiyohito ^a   Tsuji, Hirokazu ^a   Kado, Yukiko ^a   Takahashi, Takuya ^a   Kida, Yumi ^b   Ito, Masahiro ^b   Okada, Nobuhiko ^b   Nomoto, Koji ^a  

^a YAKULT CENTRAL INSTITUTE FOR MICROBIOLOGICAL RESEARCH   (Japan)

^b KITASATO UNIVERSITY   (Japan)

Author keywords

Lactobacillus; RT qPCR; Vaginal microbiota

Indexed keywords

BACTERIAL RNA; RIBOSOME RNA; PRIMER DNA;

ARTICLE; ATOPOBIUM VAGINAE; BACTERIAL COUNT; BACTERIUM DETECTION; BACTERIUM IDENTIFICATION; BIFIDOBACTERIUM; BIFIDOBACTERIUM ADOLESCENTIS; BIFIDOBACTERIUM ANGULATUM; BIFIDOBACTERIUM LONGUM; BIFIDOBACTERIUM LONGUM SUBSP. INFANTIS; CHLAMYDIA; CHLAMYDIA TRACHOMATIS; CONTROLLED STUDY; FEMALE; GARDNERELLA VAGINALIS; HUMAN; HUMAN EXPERIMENT; LACTOBACILLUS CRISPATUS; LACTOBACILLUS GASSERI; MICROBIAL DIVERSITY; MOBILUNCUS CURTISII; NONHUMAN; NORMAL HUMAN; PREVOTELLA; QUANTITATIVE ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS; SENSITIVITY ANALYSIS; STREPTOCOCCUS; VAGINA DISCHARGE; VAGINA FLORA; VAGINITIS; ACTINOBACTERIA; ADULT; ASYMPTOMATIC INFECTION; BACTERIUM; GENETICS; ISOLATION AND PURIFICATION; LACTOBACILLUS; MICROBIOLOGY; MICROFLORA; MOBILUNCUS; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION; SENSITIVITY AND SPECIFICITY; VAGINA; VAGINOSIS, BACTERIAL;

ATOPOBIUM VAGINAE; BACTERIA (MICROORGANISMS); BIFIDOBACTERIUM ADOLESCENTIS; BIFIDOBACTERIUM ANGULATUM; BIFIDOBACTERIUM LONGUM; CHLAMYDIA; GARDNERELLA VAGINALIS; LACTOBACILLUS; LACTOBACILLUS CRISPATUS; LACTOBACILLUS GASSERI; LACTOBACILLUS INERS; LACTOBACILLUS JENSENII; LACTOBACILLUS VAGINALIS; MOBILUNCUS CURTISII; PREVOTELLA; STREPTOCOCCUS;

ACTINOBACTERIA; ADULT; ASYMPTOMATIC INFECTIONS; BACTERIA; BIFIDOBACTERIUM; CHLAMYDIA TRACHOMATIS; DNA PRIMERS; FEMALE; GARDNERELLA VAGINALIS; HUMANS; LACTOBACILLUS; MICROBIOTA; MOBILUNCUS; REAL-TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION; RNA, RIBOSOMAL; SENSITIVITY AND SPECIFICITY; VAGINA; VAGINOSIS, BACTERIAL;

EID: 84922583798     PISSN: 01677012     EISSN: 18728359     Source Type: Journal    
DOI: 10.1016/j.mimet.2015.01.021     Document Type: Article

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