Identification of essential genes and synthetic lethal gene combinations in Escherichia coli K-12

Methods in Molecular Biology

Volumn 1279, Issue , 2015, Pages 45-65

  Mori, Hirotada ^a   Baba, Tomoya ^a   Yokoyama, Katsushi ^a   Takeuchi, Rikiya ^a   Nomura, Wataru ^a   Makishi, Kazuichi ^a   Otsuka, Yuta ^a   Dose, Hitomi ^a   Wanner, Barry L ^a  

^a NONE

Author keywords

ASKA collection; Escherichia coli; Essential genes; FLP recombinase; FRT; Gene deletion; Gene knockout; Homologous recombination; In frame deletion; KEIO collection; Lambda Red recombinase

Indexed keywords

ANTIBIOTIC RESISTANCE; ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIAL GENE; BACTERIUM CULTURE; ESCHERICHIA COLI K 12; GENE DELETION; KNOCKOUT GENE; LETHAL GENE; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; AMINO ACID SEQUENCE; BACTERIUM CONJUGATION; ELECTROPORATION; ESSENTIAL GENE; GENE INACTIVATION; GENETIC COMPLEMENTATION; GENETIC TRANSFORMATION; GENETICS; GENOMICS; METABOLISM; MOLECULAR GENETICS; MUTATION; NUCLEOTIDE SEQUENCE; PROCEDURES; REPRODUCIBILITY;

ESCHERICHIA COLI; ESCHERICHIA COLI K12;

PRIMER DNA; SYNTHETIC DNA;

AMINO ACID SEQUENCE; BASE SEQUENCE; CONJUGATION, GENETIC; DNA PRIMERS; ELECTROPORATION; ESCHERICHIA COLI K12; GENE KNOCKOUT TECHNIQUES; GENES, BACTERIAL; GENES, ESSENTIAL; GENES, LETHAL; GENES, SYNTHETIC; GENETIC COMPLEMENTATION TEST; GENOMICS; MOLECULAR SEQUENCE DATA; MUTATION; POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; TRANSFORMATION, GENETIC;

EID: 84922428644     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-2398-4_4     Document Type: Article

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