Expression, purification, and identification of associated proteins of the full-length hCDK12/CyclinK complex

Journal of Biological Chemistry

Volumn 290, Issue 3, 2015, Pages 1786-1795

  Bartkowiak, Bartlomiej ^a   Greenleaf, Arno L ^a  

^a DUKE UNIVERSITY MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ENZYMES; MASS SPECTROMETRY; PURIFICATION; RNA; SUBSTRATES; TRANSCRIPTION;

CONSENSUS SEQUENCE; EXPRESSION SYSTEM; FUSION PROTEINS; OVARIAN CANCERS; RECOMBINANT ENZYMES; RNA POLYMERASE II; RNA PROCESSING; TUMOR SUPPRESSORS;

PROTEINS;

CYCLIN DEPENDENT KINASE; CYCLIN DEPENDENT KINASE 12; CYCLIN K; FLAVOPIRIDOL; PROTEIN SERINE THREONINE KINASE; UNCLASSIFIED DRUG; CCNK PROTEIN, HUMAN; CDK12 PROTEIN, HUMAN; CDK2 PROTEIN, HUMAN; CDK9 PROTEIN, HUMAN; CYCLIN DEPENDENT KINASE 2; CYCLIN DEPENDENT KINASE 9; CYCLINE; GLUTATHIONE TRANSFERASE; PROTEIN BINDING; RNA;

ARTICLE; BACULOVIRIDAE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME SPECIFICITY; ENZYME SUBSTRATE; GENE CONSTRUCT; IMMUNOPRECIPITATION; MASS SPECTROMETRY; NONHUMAN; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN PHOSPHORYLATION; PROTEIN PURIFICATION; PROTEIN RNA BINDING; RNA PROCESSING; SF9 CELL LINE; AMINO ACID SEQUENCE; DRUG DEVELOPMENT; HELA CELL LINE; HUMAN; METABOLISM; MOLECULAR GENETICS; MUTATION; PHOSPHORYLATION; PROTEIN TERTIARY STRUCTURE; SEQUENCE HOMOLOGY;

EUKARYOTA;

AMINO ACID SEQUENCE; BACULOVIRIDAE; CYCLIN-DEPENDENT KINASE 2; CYCLIN-DEPENDENT KINASE 9; CYCLIN-DEPENDENT KINASES; CYCLINS; DRUG DISCOVERY; GLUTATHIONE TRANSFERASE; HELA CELLS; HUMANS; MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA; MUTATION; PHOSPHORYLATION; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; RNA; SEQUENCE HOMOLOGY, AMINO ACID; SUBSTRATE SPECIFICITY;

EID: 84922118253     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M114.612226     Document Type: Article

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