Dignam and Roeder nuclear extract preparation

Cold Spring Harbor Protocols

Volumn 4, Issue 12, 2009, Pages

  Carey, Michael F ^a   Peterson, Craig L ^a   Smale, Stephen T ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; CELL COUNT; CELL DENSITY; CENTRIFUGATION; CLINICAL PROTOCOL; CONDUCTANCE; CYTOLYSIS; CYTOMETRY; DIALYSIS; EXTRACTION; GEL MOBILITY SHIFT ASSAY; GENE INACTIVATION; HELA CELL; HOMOGENATE; HUMAN; HUMAN CELL; PROTEIN ANALYSIS; PROTEIN DEGRADATION;

EID: 72149102444     PISSN: None     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot5330     Document Type: Article

References (12)

1. Carey MF, Peterson CL, Smale ST. 2009. In vitro transcription using HeLa cell extracts and primer extension. Cold Spring Harb Protoc (this issue). doi: 10.1101/pdb.prot5331.
2. Dignam JD, Lebovitz RM, Roeder RG. 1983. Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei. Nucleic Acids Res 11: 1475-1489.
3. Ge H, Martinez E, Chiang CM, Roeder RG. 1996. Activator dependent transcription by mammalian RNA polymerase II: In vitro reconstitution with general transcription factors and cofactors. Methods Enzymol 274: 57-71.
4. Gorski K, Carneiro M, Schibler U. 1986. Tissue-specific in vitro transcription from the mouse albumin promoter. Cell 47: 767-776.
5. Harlow E, Lane D. 2006. Bradford assay. Cold Spring Harb Protoc doi: 10.1101/pdb.prot4644.
6. Lee KA, Bindereif A, Green MR. 1988. A small-scale procedure for preparation of nuclear extracts that support efficient transcription and pre-mRNA splicing. Gene Anal Tech 5: 22-31.
7. Maldonado E, Drapkin R, Reinberg D. 1996. Purification of human RNA polymerase II and general transcription factors. Methods Enzymol 274: 72-100.
8. Manley JL, Fire A, Samuels M, Sharp PA. 1983. In vitro transcription: Whole-cell extract. Methods Enzymol 101: 568-582.
9. Osborn L, Kunkel S, Nabel GJ. 1989. Tumor necrosis factor and interleukin 1 stimulate the human immunodeficiency virus enhancer by activation of the nuclear factor B. Proc Natl Acad Sci 86: 2336-2340.
10. Roeder RG. 1996. Nuclear RNA polymerases: Role of general initiation factors and cofactors in eukaryotic transcription. Methods Enzymol 273: 165-171.
11. Sambrook J, Russell DW. 2006. Estimation of cell number by hemocytometry counting. Cold Spring Harb Protoc doi: 10.1101/pdb.prot4454.
12. Stuempfle KJ, Koptides M, Karinch AM, Floros J. 1996. Preparation of transcriptionally active nuclear extracts from mammalian tissues. BioTechniques 21: 48-50.